- P-ISSN 1010-0695
- E-ISSN 2288-3339
- Log In/Sign Up
- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
Home > Article Detail
Cytotoxic effects of ethanol extracts of Saussurea lappa mediated by mitochondrial apoptotic pathway
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2004, v.25 no.4, pp.79-89
Seung-Hee,
K.
, Seong-Gyu,
K.
, Chan-Yong,
J.
, &
Chong-Hyeong,
P.
(2004). Cytotoxic effects of ethanol extracts of Saussurea lappa mediated by mitochondrial apoptotic pathway. Journal of Korean Medicine, 25(4), 79-89.
- Downloaded
- Viewed
Abstract
Saussurea lappa and Taraxacum mongolicum have been used for herbal medicinal treatments against cancers in East Asia. We performed this study to understand the molecular basis underlying the anti-tumor effects of two herbs and analyzed the effects of these medicinal herbs on proliferation and on expression of cell growth/apoptosis related molecules by using an AGS gastric cancer cell line. The treatments of Saussurea lappa dramatically reduced cell viabilities in a dose and time-dependent manner, but Taraxacum mongolicum did not. FACS analysis and Annexin V staining assay also showed that Saussurea lappa induces apoptotic cell death of AGS. Expression analyses via RT-PCR and Western blots revealed that Saussurea lappa increased expression of the p53 and its downstream effector p21Waf1, and that the both increased expression of apoptosis related Bax and cleavage of active caspase-3 protein. We also confirmed the translocation of Bax to mitochondria. Collectively, our data demonstrate that Saussurea lappa induces growth inhibition and apoptosis of human gastric cancer cells, and these effects are correlated with down- and up-regulation of growth-regulating apoptotic and tumor suppressor genes, respectively.
- keywords
- Saussurea lappa, mitochondrial pathway, apoptosis, anti-tumor effect, Bax, medicinal herbs.
Reference
1
Rivas AL. Trends in Cell Biology.1993,
2
Oren M. Wild-type p53 can down-modulate the activity of various promoters. Proc Natl Acad Sci.1991,
3
(2001) Apoptosis-regulating proteins as targets for drug discovery.,
4
(2003) Bax Plays a Pivotal Role in Thapsigargin-induced Apoptosis of Human Colon Cancer HCT116 Cells by Controlling Smac/Diablo and Omi/HtrA2 Release from Mitochondria. ,
5
Nagata S. The journal of immunology.1993,
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Effect of Do-In (Prunus persica L. BATSCH) Water Extract (PPE) on Concentration of Extracellular Acetylcholine in the Rat Hippocampus
- The Anti-nociceptive and Anti-inflammatory Effect of Achyranthes Japonica Nakai
- Effects of Bambusae concretio Silicea on Suppression of Collagenolysis and Bone Resorption in Mouse Calvarial Osteoblasts
- Inhibitory effects of transforming growth factor and Drynariae Rhizoma on leukocytosis associated with the chronic phase of arthritis in mice
- Study on the Relationship between Polymorphisms in Glutathione S-transferase and Ischemic Cerebrovascular Disease
- Correlation between Obesity and Lumbar Lordosis in Obese Pre-Menopausal Korean Females
- Effect of Saenggitang on Learning and Memory Ability in Mice
- Experimental Studies on the Effect of Gamibaegi-eum
- Cytotoxic effects of ethanol extracts of Saussurea lappa mediated by mitochondrial apoptotic pathway
- Effects of Panax Ginseng on the Sperm Motility and Spermatogenesis in the SD Rat
- Paeonia radix decreases intracerebral hemorrhage-induced neuronal cell death via suppression on caspase-3 expression in rats
- Inhibition of cell growth and induction of apoptosis by Euonymus alatus (Thunb.) Sieb in human leiomyomal smooth muscle cells
- Susceptibility of Oral Bacteria to Essential Oil of Artemisia capillaris Thunb
- A PILOT STUDY TO ASSESS THE EFFECT OF GAMI-JIWHANG-TANG ON COGNITIVE EFFECTS IN HEALTHY CHILDREN
- A PILOT STUDY FOR DEVELOPING AN ASSESSMENT SCALE FOR THE EFFECT OF HERBAL MEDICINE IN HEALTHY CHILDREN: OPEN-LABEL STUDY WITH GAMI-JIWHANGTANG
- Identification of Angelica Species by Pyrosequencing
- Analysis of Survival in 273 Terminally Ill Cancer Patients Treated with Traditional Oriental Therapies
- Effects of Armillaria mellea Extract on Macrophage and NK Cell Activity
- A Study on the Clinical Features of Ordinary Sleeping Patterns Based on the Sasang Constitution, Using the Logistic Regression
- Screening for Various Herb Medicines Extracts against HSV-1,2
- Novel Therapeutic Approach toward Inflammatory Diseases: Targeting Transglutaminase 2
- Effects of Palmijihwangtang (PMT) and Exercise on Glucose Metabolism in Myocardium Cell Membrane and Pancreas β-Cell of Zucker Diabetic Fatty Rats
- Study on the Relationship between Candidate Genes of Cerebral Infarction and Sasang Constitution
- A Clinical Study of Electroacupuncture on an Overactive Bladder
- A Case Report for One Case of Thyrotoxic Periodic Paralysis Patient
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.