- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Identification of Angelica Species by Pyrosequencing
대한한의학회지 / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2004, v.25 no.4, pp.147-151
(대구한의대학교)
(대구한의대학교)
(경희대학교)
(경희대학교)
(세명대학교)
(대구한의대학교)
(경희대학교)
(경희대학교)
(세명대학교)
Jung-Chul,
S.
,
Sang-Won,
H.
,
Ho-Young,
C.
,
Young-Ju,
C.
,
&
임강현.
(2004). . 대한한의학회지, 25(4), 147-151.
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Abstract
Objective : Angelica species are some of the most medicinally important materials in Oriental medicine. This study was performed to determine if Angelica species could be identified by genetic analysis and to verify Pyrosequencing analyses, which were used to assess genetic variation. Method : The DNAs of Angelica acutiloba, Angelica gigas and Angelica sinensis were extracted. We have investigated the typing of single-base variations of Angelica species in DNA by using Pyrosequencing. Result : Angelica gigas showed a different pattern compared with Angelica acutiloba and Angelica sinensis. The peak of Angelica gigas was very weak in the second C nucleotide base compared with that of the others. The peak of Angelica acutiloba was present in the fourth C nucleotide base compared with that of the others. From these results we verified that our Angelica species-specific sequencing primer was well designed. Conclusion : Pyrosequencing analysis might be able to provide the identification of the Angelica species.
- keywords
- Angelica acutiloba, Angelica gigas, Angelica sinensis, Pyrosequencing, Identification, Angelica acutiloba, Angelica gigas, Angelica sinensis, Pyrosequencing, Identification
참고문헌
1
(2001) Herbal Pharmacology, Jibmoon Press
2
(2004) Natural medicine: the genus Angelica,
3
(2003) Molecular genetic and chemical assessment of radix Angelica(Danggui) in China,
4
(2001) Alternative medicine: herbal drugs and their critical appraisal--part II.,
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(2002) Pharmacokinetics and bioavailability of herbal medicinal products,
6
(2003) Analysis of an interleukin-6 gene promoter polymorphism in women with endometriosis by pyrosequencing,
7
(2002) Estimation of single nucleotide polymorphism allele frequency in DNA pools by using Pyrosequencing,
8
(2003) Molecular haplotype determination using allele-specific PCR and pyrosequencing technology. Genomics,
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