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ISSN : 1225-3480
Genomic DNA isolated from two geographical ark shell (Scapharca subcrenata) populations was amplified several times by PCR reactions. The ark shell population from Daecheon (ASPD) and from Wonsan (ASPW) in the West Sea and the East Sea of Korean Peninsula, respectively, obtained. The seven arbitrarily selected primers OPA-05, OPA-11, OPB-09, OPB-11, OPB-14, OPC-18 and OPD-07 were shown to generate the loci observed per primer, shared loci by each population, specific loci, unique shared loci to each population and shared loci by the two populations which could be clearly scored. Here, 862 loci were identified in the ASPD population, and 1,191 in the ASPW population: 137 specific loci (15.9%) in the Daecheon population and 84 (7.1%) in the Wonsan population. 407 shared loci by each population, with an average of 58.1 per primer, were observed in the ASPD population. 473 shared loci by each population, with an average of 67.6 per primer, were identified in the ASPW population. The numbers of specific loci in the ASPD and ASPW population were 137 and 84, respectively. Consequently, the average bandsharing value of individuals within the ASPW population was much higher than in the ASPD population. The bandsharing value between individuals’ no. 08 and no. 13 was 0.628, which was the highest measured between the two geographical populations. The dendrogram obtained by the seven primers indicated three genetic clusters: cluster 1 (DAECHEON 01 ~ DAECHEON 11), cluster 2 (WONSAN 12 and 14) and cluster 3 (WONSAN 13, 15, 16, 17, 18, 19, 20, 21 and 22). The genetic distance between the two geographical populations ranged from 0.043 to 0.499. Especially, individual no. 10 of Daecheon population was most distantly related to no. 14 of Wonsan population (genetic distance = 0. 499).
Bae, T. J. 1998. Processing suitability of canned ark shell. Korean Journal of Food and Nutrition 11 (2) : 237~242.
Callejas, C. and M. D. Ochando. 1998. Identification of Spanish barbel species using the RAPD technique. Journal of Fish Biology 53: 208-215.
Chenyambuga, S. W., O. Hanotte, J. Hirbo, P. C. Watts, S. J. Kemp, G. C. Kifaro, P. S. Gwakisa, P. H. Petersen, and J. E. O. Rege. 2004. Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers. Asian-Australasian Journal of Animal Sciences 17: 445-452.
Huang, B. X., R. Peakall, and P. J. Hanna. 2000. Analysis of genetic structure of blacklip abalone (Haliotis rubra) populations using RAPD, minisatellite and microsatellite markers. Marine Biology 136: 207-216.
Jeffreys, A. J. and D. B. Morton. 1987. DNA fingerprints of dogs and cats. Animal Genetics 18: 1-15.
Jung, H. T, J. Kim and S. D. Choi. 2004a. Phylogenetic relationship of the five Korean clams, Bivalvia, Veneroida according to morphological characters. Journal of Aquaculture, 17 (3) : 197-208.
Jung, H. T, J. Kim, J. A. Shin, H. Y. Soh and S. D. Choi. 2004b. Genetic relationship of the five venerid clams (Bivalvia, Veneridae) in Korea. Journal of Aquaculture, 17 (4) : 251-257.
Kim, J. Y., C. Y. Park, and J. M. Yoon. 2004. Genetic differences and DNA polymorphism in oyster (Crassostrea spp.) analysed by RAPD-PCR. Korean Journal of Genetics 26: 123-134.
Kim, K. S., J. H. Lim, T. J. Bae and C. K. Park. 2002. Characteristics of food components in granular ark shell. Journal of Korean Fisheries Society 35 (5) : 512~518.
Kim, S. K., Y. H. Jung, S. H. Han, Y. S. Oh, M. H. Ko and M. Y. Oh. 2000. Phylogenetic relationship among Haliotis spp. distributed in Korea by the RAPD analysis. Korean Journal of Genetics 22: 43-49.
Liu, Z., P. Li, B. J. Argue, and R. A. Dunham. 1998. Inheritance of RAPD markers in channel catfish (Ictalurus punctatus), blue catfish (I. furcatus) and their F1, F2 and backcross hybrids. Animal Genetics 29: 58-62.
Muchmore, M. E., G. W. Moy, W. J. Swanson, and V. D. Vacquier. 1998. Direct sequencing of genomic DNA for characterization of a satellite DNA in five species of Eastern Pacific abalone. Molecular Marine Biology and Biotechnology 7 (1) : 1-6.
Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583-590.
Nozaki, T., K. Mishiba, M. Mii, and T. Koba. 2000. Construction of synteny groups od Brassica alboglabra by RAPD markers and detection of chromosome aberrations and distorted transmission under the genetic background of B. campestris. Theoretical and Applied Genetics 101: 538-546
Park, C. K. 1999. Seasonal variation of proximate composition in edible portion of ark shell (Scapharca subcrenata). Journal Korean Society of Food Sciences and Nutrition 28 (6) : 1226~1229.
Park, C. K. 2000. Seasonal variation of proximate composition in ark shell (Scapharca subcrenata) Tissues Journal Korean Fish. Society of Food Sciences and Nutrition 29 (1) : 10~14.
Tassanakajon, A., S. Pongsomboon, P. Jarayabhand, S. Klinbunga, and V. Boonsaeng. 1998. Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA analysis. Journal of Marine Biotechnology 6: 249-254.
Wang, Y., L. Liang, J. Shi and G. Jiang. 2005. Study on the contamination of heavy metals and their correlation in mollusks collected from coastal sites along the Chinese Bohai Sea. Environment International 31: 1103-1113.
Wang, Y., R. Yang and G. Jiang. 2006. Investigation of Organochlorine pesticides (OCPS) in mollusks collected from coastal sites along the Chinese Bohai Sea from 2002 to 2004. Environment Pollution XX: 1-7.
Welsh, J. and M. McClelland. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research 18: 7213-7218.
Welsh, J., C. Petersen, and M. McClelland. 1991. Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping. Nucleic Acids Research 19: 303-306.
Yoke-Kqueen, C. and S. Radu. 2006. Random amplified polymorphic DNA analysis of genetically modified organisms. Journal of Biotechnology 127: 161-166.
Yoon, J. M. and G. W. Kim. 2001. Randomly amplified polymorphic DNA-polymerase chain reaction analysis of two different populations of cultured Korean catfish Silurus asotus. Journal of Biosciences 26: 641-647.
Yoon, J. M. and H. Y. Park. 2002. Genetic similarity and variation in the cultured and wild crucian carp (Carassius carassius) estimated with random amplified polymorphic DNA. Asian-Australasian Journal of Animal Sciences 15: 470-476.
Yoon, J. M. and Y. H. Kim. 2003a. Wide marsh clam (Corbicula sp.) populations from three sites analysed by RAPD-PCR-AGE. Bulletin of Electrochemistry 19: 337-348.
Yoon, J. M. and G. W. Kim. 2003b. Genetic differences between cultured and wild penaeid shrimp (Penaeus chinensis) populations analysed by RAPD-PCR. Korean Journal of Genetics 25: 21-32.
Yoon, J. M. and J. Y. Kim. 2004. Genetic differences within and between populations of Korean catfish (S. asotus) and bullhead (P. fulvidraco) analysed by RAPD-PCR. Asian-Australasian Journal of Animal Sciences 17: 1053-1061.
Yoon, J. M. and S. Y. Park. 2006. Genetic differences and variation in two purplish Washington clam (Saxidomus purpuratus) populations from South and North Korea. Korean Journal of Malacology 22: 97-108.