ISSN : 1225-3480
본 연구에서는 높은 변이를 나타내는 6개의 Microsatellite DNA 유전표식을 이용하여 독도지역에 서식하고 있는 자연산 전복집단의 유전적 다양성 및 집단구조를 파악하여 동 서 남해의 6개 지역집단과 비교 분석하였다. 그 결과 독도지역의 유전적 다양성은 6개 지역집단보다 높게 나타났으며 유전적 거리에 의한 유연관계 분석 결과에 있어서도 이들 집단과는 독립된 집단으로 나타났다. 이러한 결과는 6개 지역집단에 있어 방류전복의 높은 혼획비율에 의한 자연산 집단의 유전적 다양성이 축소되어진 결과라고 생각되어진다. 따라서 독도집단의 높은 유전적 다양성을 유지하고 보존하기 위해서는 이 지역의 유전적 다양성을 고려한 체계화된 전복종묘의 방류가 이루어져야하며, 방류를 한 이후에도 지속적인 유전학적 모니터링이 필요하다고 생각되어진다.
This study was conducted to investigate the genetic characteristics of wild population of Pacific abalone, Haliotis discus hannai in Dokdo island. We used six polymorphic microsatellite marker to investigate the genetic diversity and population structure. The loci Hdh1321 and Hdh512 had the highest number of allele (34 and 22 respectively) and loci Hdh145 and Awb083 had the lowest (5 and 7 respectively). The mean number of allele per locus was 14.8. The average observed and expected heterozygosities were 0.664 and 0.824 respectively, and the average FIS was 0.195. We compared the population genetic parameters of Dokdo population with previously published data of the same species. At the result, the parewise FST test showed significant difference between the Dokdo population and six populations (published data), suggesting that the genetic relationship of Dokdo population was separated from six populations.
김훈수, 최병래, 1981. 울릉도 및 독도의 해양 무척추동물상. 한국자연보존협회 조사보고서. 19, 193-200.
해양수산부. 2000. 독도 생태계 등 기초조사 연구. 11-649.
Dieringer D., Schlötterer C. (2002) Microsatellite analyser (MSA): a platform independent analyser tool for large microsatellite data sets, Molecular Ecology Note 3: 167-169.
Evans B., White R. W. G., Elliott N. G. (2000) Characterization of microsatellite loci in Austrailian blacklip abalone (Haliotis rubra, Leach). Molecular Ecology, 9: 1183-1184.
Goudet J. (2002) FSTAT version 2.9.3.2: a program to estimate and test gene diversity and fixation indices.
Hara M., Sekino M. (2005) Genetic difference between Ezo-awabi Haliotis discus hannai and Kuro-awabi H. discus discus population: microsatellite-based population analysis in Japanese abalone. Fisheries Science, 71: 754-766.
Hunang B., Hannna P. J. (1998) Identification of three polymorphic microsatellite loci in blacklip abalone, Haliotis rubra (Leach) and detection in other abalone species, journal of Shellfish Res, 17: 795-799.
Jeong D. S., Park C. J., Jeon C. Y. (2008) Genetic variability and population structure of Pacific abalone microsatellite DNA markers, Journal of the korean fisheries society, 41(6): 466-470.
Kang, R. S., Won K. S., Hong K. P., Kim J. M. (2001) Population studies on the Kelp Ecklonia cava and Eisenia bicyclis in Dokdo, korea, 16(2): 209-215.
Li Q., Park C., Kijima A. (2002) Isolation and characterization of microsatellite loci in the Pacific abalone, Haliotis discus hannai, Journal of Shellfish Research, 21: 811-815.
Marshall T. C., Slate J., Kruuk L., Pemberton J. M. (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Molecular Ecology, 7: 639-655.
Miller K. M., Laberee K., Kaukinen K. H., Li S., Withler R.E. (2001) Development of microsatellite loci in pinto abalone (Haliotis kamtschatkana). Molecular Ecology Notes, 1: 315-317.
Nei M., Tajima, F., Tateno Y. (1983) Accuracy of estimated phylogenetic trees from molecular data. Journal of Molecular Evolution, 19: 153-170
Park C. J., Li Q., Kobayashi T., Kijima A. (2003) Characterization novel microsatellite DNA marker in the Pacific abalone, Haliotis discus hannai, Fish genetics and Breeding science, 33: 19-24.
Raymond M., Rousset F. (1995) GenePop (version 1.2): population genetics software for exact tests and ecumenicism. Journal of Heredity, 86: 248-249.
Ren P., Wang Z., Yao C., Liu Y., Ke C. (2008) Development of 11 polymorphic microsatellite loci in the small abalone (Haliotis diversicolor Reeve), 8: 1390-1392.
Sekino M., Saido T., Fujita T., Kobayashi T., Takami H. (2005) Microsatellite DNA markers of Ezo abalone (Haliotis discus hannai): a preliminary assessment of natural populations sampled from heavily stocked areas, Aquaculture, 243: 33-47.
Sekino M., Kobayashi T., Hara M. (2006) Segregation and Linkage Analysis of 75 Novel Microsatellite DNA markers in Pair Crosses of Japanese Abalone (Haliotis discus hannai) Using the 5`-Tailed Primer Method. Marine Biotechnology, 8: 453-466.
Sekino M., Hara M. (2007) Individual assignment tests proved genetic boundaries in a species complex of Pacific abalone (genus Haliotis). Conservation Genetics, 8: 823-841.
Selvamani M. J. P., Degnan S. M., Paetkau D., Degnan B. M. (2000) Highly polymorphic microsatellite loci in the Heron Reep population of the tropical abalone, Haliotis asinina. Molecular Ecology, 9: 1184-1185.
Suenaga E., Nakamura H. (2005) Evolution of tree methods for effective extraction of DNA from human hair, Journal of chromatography B, 820: 137-141.
Sun X. Q., Zhen M. G., Yang G. P. (2007) Development of 15 polymorphic genic microsatellite DNA markers of Pacific abalone Haliotis discus hannai. Molecular Ecology Notes, 7: 604-606.
Walsh P. S., Metzger D. A., Higuchi R. (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material, Biotechniques, 10: 506-513.