ISSN : 1225-3480
96시간 동안 고염분에 노출시킨 일본재첩, Corbicula japonica의 LC_(50)은 19.550 psu였다. 0, 5, 10, 20 psu에 7일 동안 노출시킨 실험개체들은 실험종료시기에 각각 95%,80%, 35%, 10%의 개체들이 생존하였다. 일반적인 일본재첩의 아가미는 좌․우 한 쌍으로서 내부판의 면적은 외부판보다1.37배 넓었다 (p < 0.001). 아가미의 새엽에는 그 위치에 따라 상부에 정단섬모상피세포 (7 μm), 정단측면섬모상피세포(5 μm), 후정단측면섬모상피세포 (3 × 8 μm), 측면섬모상피세포 (5 μm) 가 존재하고, 새엽의 중간부분에는 혈림프동을 둘러싸고 있는 혈관상피세포가 존재하며, 하부에는 새엽하부상피세포가 존재하고 있었다. 새엽의 하부에 주로 존재하는분비세포들은 전자밀도가 낮은 섬유성의 분비과립을 가지고있었다. 5 psu에 7일 동안 노출된 일본재첩의 아가미는 부분적인 섬모의 탈락과 glycogen 과립이 다수 관찰되었다. 10psu에 노출된 개체들은 일부 새엽의 상피세포가 파괴되었으며, 미토콘드리아를 포함한 세포소기관 또한 파괴되었다. 섬모들은 원형질막이 팽창되었고 미세융모를 연결시키는 당질층의파괴도 관찰되었다. 20 psu에 노출된 일본재첩의 아가미는 새엽섬모상피세포 핵비대, 세포소기관의 파괴, 세포질내glycogen 과립의 침적과 공포형성이 관찰되었고, 50% 이상의새엽은 새엽상피층의 탈락으로 인하여 키틴질 기둥이 모두 노출되었다. 따라서 이러한 섬모와 상피세포의 파괴는 생리활동의 장애를 유발시키고, 개체 사망의 직접적인 원인으로 작용할것이다.
This study was performed to observe ultrastructure of the gill and to ascertain the effect of salinity on histopathological and ultrastructural changes in the gill of the Japanese clam, Corbicula japonica. Experimental period was 7 days. Experimental groups consisted of control, 5, 10, 20 psu. LC_(50) (96 h.) by the probit was 19.55 psu. Mortality was significantly different from the control (p < 0.05). Inner demibranch of the gill of C. japonica was wider 1.37 times than outer demibranch (p < 0.001). The filament zone on the plica can be distinguished by the six epithelial celll cell; frontal ciliated epithelium (7 μm), latero-frontal ciliated epithelium (5 μm), postlatero-frontal epithelim (3 x 8 μm), and lateral ciliated epithelium (5 μm) in the frontal zone, endothelial cellin the intermediate zone, and abfrontal cell in the abfrontal zone. It had one type of secretory cell that was filled with fibrous substances of low electron density. The gill of C. japonica exposed to 5 psu for 7 days was observed partially disappearance of the cilia, and glycogen granule in the filament. In the 10 psu, gill appeared partially modification of epithelial cell and destruction of the glycocalyx. Gill exposed to 20 psu was extended nuclus of the ciliated epithelial cell, destruction of the organelles, and observed glycogen granules infiltration and numerous vacuoles. Moreover, more than 50% filaments were observed that come out chitinous rod from disappearance of epithelial cell in the filament. Therefore, the destruction of the cilia and epithelial cell induce physiological activity and it may be leading directly to death.
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