ISSN : 1225-3480
TBTCl에 36주 동안 노출된 대복 (Gomphina veneriformis) 의 미세구조적인 변화를 전자현미경으로 관찰하였다. 대복의 외투막은 가장자리에 4개의 주름을 가지며, 내부와 외부상피층이 단층의 원주형 상피세포, 섬모세포, 분비세포로 구성되어 있었다. 이들 상피층은 혈림프동이 존재하는 결합조직층을 둘러싸고 있었다. 대복의 외투막은 TBTCl 노출12주째 내부상피층에 존재하는 섬모의 수가 감소하였으며, 혈림프동의 확장과 함께 격막의 파괴가 관찰되었다. 노출 20주째 세포질내의 공포형성, 핵응축과 같은 변화가 관찰되었고,결합조직층에서는 근섬유다발의 분절이 부분적으로 관찰되었다. 노출 28주째 상피층의 부분적인 붕괴, 섬모의 변화 및 섬모세포에 존재하는 미세구조가 괴사되었다. 결합조직층에 존재하는 다수의 근형질막은 팽창되었으며, 막의 내부에는 근섬유들이 대부분 파괴되어 막 주변에 근섬유 잔여물들이 관찰되었다. 노출 36주째에는 섬모들은 모두 탈락되고, 핵 응축되고,이형소체의 증가가 나타나는 퇴행성 상피세포가 나타났다. 외부상피층의 상피세포들이 괴상되어 핵이 파괴되고, 세포질 내에는 다수의 용해소체들이 가득 차 있었으며, 미세융모들은 대부분 소실되었다. 따라서 만성적인 TBTCl 노출은 대복의 외투막은 각장성장 및 생리적인 기능의 장애를 유발하는 것으로생각된다.
Ultrastructural changes in the mantle of the equilateral venus, Gomphina veneriformis exposed to tribultyltin chloride (TBTCl) for 36 weeks were observed. The mantle had 4-folds and its epidermal layer consisted of simple columnar epithlial cells and ciliated cells and secretory cells. Inner and outer epidermal layer covered connective tissue. The mantle exposed to TBTCl at 12 weeks decreased cilia in the inner epidermal layer, and observed extension of the hemolymph sinus and destruction of the septum. At 20 weeks, it revealed vacuole formation and pycnosis in the cytoplasm, and scattered muscular fiber. After 28 weeks of exposure, the mantle revealed partially destructive changes epidermal layer, isolation of basal body, and scattered rootlet complex and basal foot. The Sarcolemma had extension membrane and debris fiber. At 36 weeks, it observed degenerative cells that it revealed disappearance of the cilia, atrophic nucleus, poorly membrane and destruction of the cresternae in the mitochondria, and increasing heterophagosome. The outer epithalial cell had nectoric nuclus, numeous lysosome and disappearance of the microvilli. Therefore, results of this study suggested that chronical TBTCl exposure in the Gomphina veneriformis induced shell growth, and physiological function disorder of the mantle with histopathological changes.
Alzieu, C. (2000) Environmental impact of TBT: the French experience. Science of the Total Environment, 258: 99-102.
Bubel, A. (1973) An electron mocroscope investigation of the cells lining the outer surface of the mantle in some marine molluscs. Marine Biology, 21: 245-225.
Bubel, A. (1984) Epidermal cells. In; Biology of the Integument, 1 Invertebrates, (ed. by Bereiter-Hahn, J., Matoltsy, A.G. and Richards, K.S.). pp. 400-477. Springer-Verlag, New York.
Bubel, A. (1989) Microstructure and function of cells; Electron micrographs of cell ultrastructure. pp. 1-271, Ellis Horwood Limited. Chichester.
Champ, M.A. (2000) A review of organotin regulatory strategies, pending actions, related costs and benefits. Science of the Total Environment, 258: 21-71.
Clavell, C., Seligman, P.F. and Stang, P.M. (1986) Automated analysis of organotin compounds : A method for monitoring butyltins in the marine environment. In: Proceeding of the Oceans, pp. 1152-1154, ′86 Conference, Organotin Symposium, 4.
Cormack, D.H. (2001) Introduction to histology. In: Essential histology. Second Edition. pp. 1-23. Lippincott Williams & Wilkins, Maryland.
Galtsoff, P.S. (1964) The American oyster Crassostrea virginica Gmelin. Fishery Bulletin of the Fish and Wildlife Service, Vol. 64. pp. 480. United States Government Printing Office, Washington, D.C.
Gauthier-Clerc, S., Pellerin, J., Blaise, C. and Gagné, F. (2002) Delayed gametogenesis of Mya arenaria in the Saguenay fjord (Canada): a consequence of endocrine disruptors? Comparative Biochemistry and Physiology, C: Comparative Pharmacology, 131: 457-467.
Ghadially, F.N. (1997) Ultrastructural pathology of the cell and matrix (4th edition). pp. 1414. A Hoder Arnold Publication, Boston.
Gregory, M.A., George, R.C. Marshall, D.J. Anandraj, A. and Mcclurg, T.P. (1999) The effects of mercury exposure on the surface morphology of gill filaments in Perna perna (Mollusca: Bivalvia). Marine Pollution Bulletin, 39: 116-121.
Halldórsson, H.P., Svavarsson, J. and Granmo, Å. (2005) The effect of pollution on scope for growth of the mussel (Mytilus edulis L.) in Iceland. Marine Environmental Research, 59: 47-64.
Hinch, S.G. and Stephenson, L.A. (1987) Size-and age-specific patterns of trace metal concentrations on fresh water clams from on acid-sensitive and a circumneutral lake. Canadian Journal of Zoology, 65: 2436-2442.
Ju, S.M., Lee, J.W., Jin, Y.G., Yu, J. and Lee, J.S. (2006) Effect of zinc bioaccumulation on survival rate, activity, growth and organ structure of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). Journal of Environmental Toxicology, 21: 115-126.
Kannan, K., Senthilkumer, K., Logannathan, B.G., Takahashi, S., Odell, D.K. and Tanade, S. (1997) Elevated accumulation of tributyltin and its breakdown products in bottle nose dolphins (Tursiops truncatus) found stranded along the U.S. Atlantic and Gulf coasts. Environmental Science & Technology, 31: 296-301.
Kawaguti, S. and Ikemoto, N. (1962) Electron microscopy on the mantle of a bivalve, Fabulina nitidula. Biological Journal of Okayama University, 8: 1-20.
Kim, S.Y. and Lee, T.Y. (1988) The effects of pollutants effluent from a steam-power plant on coastal bivalves. Oceanology Research, 10(1): 47-56.
Krigman, M.R. and Silverman, A.P. (1984) General toxicology of tin and its organic compounds. Neurology Toxicology, 5: 129-140.
Lee, J.S., Joo, J.Y. and Park, J.J. (2007) Histology and ultrastructure of the mantle epidermis of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). Journal of Shellfish Research, 26: 413-421.
Machin, J. (1977) Role of Integument in Molluscs. In; Transport of ions and water in animals, (ed. by Gupta, B.L., Moreton, R.B. Oschman, J.L. and Wall, B.J.). pp. 735-762. Academic Press, New York.
Mercier A, Pelletier, E. and Hamel, J.F. (1994) Metabolism and subtle toxic effects of butyltin compounds in starfish. Aquatic Toxicology, 28: 259-273.
Morrison, C.M. (1993) Histology and cell ultrastucture of the mantle and mantle lobes of the eastern oyster, Creassostrea virginica (Gmelin): a summary atlas. American Malacological Bulletin, 10(1): 1-24.
Otludil, B., Cengiz, E.I., Yildirim, M.Z., Unver, O. and Ünlü, E. (2004) The effects of endosulfan on the great ramshorn snail, Planorbariua corneus (Gastropoda, Pulmonata): a histopathological study. Chemosphere, 56: 707-716.
Oyama, Y., Ueha, T. and Hayashi, A. (1993) Effects of tri-n-butyltin on intracellular Ca2+ concentration of rat cerebellar neurons. European Journal of Pharmacology, 248: 89-93.
Park, J.J. and Lee, J.S. (2008) Tributyltin chloride (TBTCl) toxicity on the growth and mantle structure of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). The Korean Journal of Malacology, 24(3): 229-241.
Park, J.J., Lim, K.H., Park, J.C., Jeong, S.A., Kwak, I.S., Cho, H.S., Shin, H.C. and Lee, J.S. (2006) The effect of long-term cadmium exposure on the organ structure of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). Bulletin of the Fisheries Science Institute Chonnam National University, 15: 10-20.
Pavelka, M. and Roth, J. (2005) Functional ultrastructure; An atlas of tissue biology and pathology. pp. 326. New York, Springer.
Pekkarinen, M. (1996) Scanning electron microscopy, whole-mount histology, and histochemistry of two Anodontine glochidia (Bivalvia: Unionidae). Journal of Zoology, 74(11): 1964-1973.
Quinn, B., Gagné, F., Costello, M., McKenzie, C., Wilson J. and Mothersill, C. (2004) The endocrine disrupting effect of municipal effluent on the zebra mussel (Dreissena polymorpha). Aquatic Toxicology, 66: 279-292.
Rasmussen, L.P.D., Hage, E. and Karlog, O. (1983) Light and electron microscopic studies of the acute and chronic toxic effects of N-nitoroso compounds on the marine mussel, Mytilus edulis (L). Ⅱ. N-methyl-N-nitro-N-nitrodoguanidine. Aquatic Toxicology, 3: 301-311.
Regoli, F. and Orlando, E. (1994) Accumulation and subcelluar distribution of metals (Cu, Fe, Mn, Pb and Zn) in the Mediterranean mussel Mytilus galloprovincialis during a field transplant experiment. Marine Pollution Bulletin, 28(10): 592-600.
Saleuddin, A.S.M. (1974) An electron microscopic study of the formation and structure of the periostracum in Astarte (Bivalvia). Canadian Journal of Zoology, 52: 1463-1471.
Stephenson, M.D. (1991) A field bioassay approach to determining tributyltin toxicity to oysters in California. Marine Environmental Research, 32: 51-59.
Thain, J.E. and Waldock, M.J. (1986) The impact of tributyltin (TBT) antifouling paints on mollluscan fisheries. Water Science and Technology, 18: 193-202.
U.S EPA. (2003) Ambient aquatic life water quality criteria for tritutyltin (TBT)-final. pp. 1-129. Unites States Environmental Protection Agency, Washington DC.
Waldock, M.J. and Thain, J.E. (1983) Shell thickening in Crassostrea gigas: Organotin antifouling or sediment induced? Marine Pollution Bulletin, 14: 411-415.
Watling, H.R. and Watling, R.J. (1982) Comparative effects of metals on the filtering rate of brown mussel, Perna perna. Bulletin of Environmental Contamination and Toxico, 29: 651-657.
Wester, P.W., Canton, J.H., Van Iersel, A.A.J., Krajnc, E.I. and Vaessen, H.A.M.G. (1990) The toxicity of bis(tri-n-butyltin) oxide (TBTO) and di-n-butyltindichloride (DBTC) in the small fish species Oryzias latipes (medaka) and Poecilia reticulata (guppy). Aquatic Toxicology, 16: 53-72.
Yoshizuka, M., Haramaki, N., Yokoyama, M., Hara, K., Kwahara, A., Umezu, Y., Araki, H., Mori, N. and Fujimoto, S. (1991) Corneal edema induced by bis (tributyltin) oxide. Archives of Toxicology, 65: 651-655.
환경부 (2003) 환경부 고시 제 2003-163호. 제조․수입 또는 사용을 금지하거나 제한하는 화학물질, pp. 1-7. 2003. 9.16.
