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  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

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북방전복 Haliotis discus hannai의 염분 변화에 따른 생리적 반응

Physiological responses in abalone Haliotis discus hannai with different salinity

초록

염분변화에 따른 북방전복의 생리적 반응을 조사하기 위하여 고염분 및 저염분의 농도에 따라 생존율, 삼투질농도, 대사율 및 조직학적 반응을 분석하였다. 염분변화에 따른 북방전복의 생존율은 37 psu에서는 100%였다. 반면 19.8 psu에서 노출 6일째, 12.8 psu 이하에서는 노출 24시간 이내 모두 사망하여 북방전복의 7-days LS50은 24.9 psu (20.1-28.2 psu)를 나타내었다. 염분변화에 따른 호흡률은 대조구에 비해 감소하였으며, 반면 암모니아질소 배설률은 증가하였다. 삼투질농도는 염분 26.8 psu이상에서는 1시간 이내 순치하였다. 북방전복 발근육의 조직학적 반응은 염분감소에 따라 혈림프동의 확장, 상피세포의 괴사 및 식세포 증가 등의 현상이 관찰되었다.

keywords
Haliotis discus hannai, Physiological response, salinity, LS50, Histological response

Abstract

This study investigated survival rate, osmorality, respiration and excretion rate and histological response with change of salinity in the abalone, Haliotis discus hannai at 24 ± 1℃. Survival rate was 100% at 37.0 psu whereas all died after 6 days at 19.8 psu and within 24 hours at 12.8 psu respectively. The 7-day median lethal limit (7day-LS50) was 20.1-28.2 psu with confidence limits of 20.1-28.2 psu. Respiration rate in low salinity groups was lower than control group, but ammonia excretion rate was more increased in comparison to control group. Osmorality was acclimated within 1 hour at above of 26.8 psu but others could not acclimated at each experimental salinity. Histological observation of foot muscle showed hemolymph sinus distension, epidermal destruction and increased infiltration of phagocytic hem

keywords
Haliotis discus hannai, Physiological response, Salinity, <TEX>$LS_{50}$</TEX>, Histological response

참고문헌

1.

Almada-villela P.C. (1984) The effects of reduced salinity on the growth of small Mytilus edulis. Journal of Marine Biological Association United Kingdum, 64: 171-182.

2.

Bohle B. (1972) Effects of adaptation to reduced salinity on filtration activity and growth of mussels (Mytilus edulis). Journal of Experimental Marine Biological Ecology, 10: 41-49.

3.

Chen J.C. and W.C. Chen. (2000) Salinity tolerance of Haliotis diversicolor supertexta at different salinity and temperature levels. Aquaculture, 181: 191-203.

4.

Cheng W, Yeh S.P, Wang C.S and Chen J.C. (2002) Osmotic and ionic changes in Taiwan abalone Haliotis diversicolor supertexta at different salinity levels. Aquaculture, 203: 349-357.

5.

Cho S.H., Park J.E., Kim C.I., Yoo J.H., Lee S.M. and Choi C.Y. (2006) Effect of the various sources of dietary additives on growth, body composition and shell color of abalone Haliotis discus hannai. Journal of Aquaculture, 19(4): 275-280.

6.

Finney D.J. (1971) Probit Analysis, 3rd ed. Cambridge University Press. London, pp. 333.

7.

Hand S.C. and Stickle W.B. (1977) Effects of tidal fluctuations of salinity on pericardial fluid composition of the American Crassostrea virginica. Marine Biology, 42: 259-271.

8.

Jeong S.C., Jee Y.J., Son P.W. (1994) Indoor Tank culture of the abalone Haliotis discus hannai. Ⅰ. Effects of tank shape and stocking density on the growth of young abalone. Journal of Aquaculture, 7(1): 9-20.

9.

Kim T.H., Yang M.H., Choe M.K., Han S.J. and Yeu I.K. (2005) Physiological studies on acute water-temperature stress of juvenile abalone, Haliotis discus hannai. Journal of Aquaculture, 18(1): 7-12.

10.

Kim T.H., Kim K.J., Choe M.K. and Yeo I.K. (2006) Physiological changes of junvile abalone, Haliotis sieboldii exposed to acute water-temperature stress. Journal of Aquaculture, 19(2): 77-83.

11.

Kinne, O. (1966) Physiological aspects of animal in estuaries with special reference to salinity. Neth. J. Sea Res., 3: 222-244.

12.

Livingstone, D.R., Widdow, J. and Feith, P. (1979) Aspects of nitrogen metabolism of the common mussel Mytilus edulis: adaptation to abrupt and fluctuating changes in salinity. Marine Biology, 53: 41-45.

13.

Moon T.S. and Shin Y.K. (2010) Efffects of salinity on survival and metabolism of ark shell, Tegillarca granosa. Korean Journal of Malacology, 26(3): 171-177.

14.

Navarro J.M. and C.M. Gonzalez. (1998) Physiological responses of the Chilean scallop Argopecten purpuratus to decreasing salinities. Aquaculture, 167: 315-327.

15.

Park I.S., Im J.H., Lee Y.D., Kim B.L., and Han S.J. (2003) A temperature -dependent index of mitotic interval in Haliotis gigantea and Haliotis discus. Korean Journal of Genetics, 25(1): 15-19.

16.

Pierce S.K. and Greenberg M.J. (1972) The nature of cellular volume regulation in marine bivalves. Journal of Experimental Biology, 57: 681-692.

17.

Sadok S., Uglow R.F. and Haswell S.J. (1997) Haemolymph and mantle fluid ammonia and ninhydrin positive substance variation in salinity–challenged mussels (Mytilus edulis L.). Journal of Experimental Marine Biological Ecology, 212: 195-212.

18.

Sastry A.N. and Vargo S.L. (1977) Variations in the physiological response of crustacean larvae to temperature. In: Vernberg, F.J., Calabrese, A., Thurberg, F.P., Vernberg W.B.(Eds.), Physiological response of marine biota to pollutants. Academic Press, New York., pp. 410-424.

19.

Shin Y.K., Kim Y., Chung E.Y. and Hur S.B. (2000) Temperature and salinity tolerance of the manila clam, Ruditapes philippinarum, Journal of Korean Fisheries Science, 33(3): 213-218.

20.

Shin Y.K., Kim B.H., Oh B.S., Jung C.G., Sohn S.G. and Lee J.S. (2006) Physiological responsses of the ark shell Scapharca broughtonii (Bivalvia: Arciddae) to decreases in salinity. Journal of Fisheries Science and Technology, 9(4): 153-159

21.

Shin Y.K., Lee W.C., Jung R.H., Kim S.Y. and Park J.J. (2009) Survival of the ark shell Scapharca subcrenata and physiological and histological changes at decreasing salinity. Fisheries and Aquatic Science, 12(3): 209-218.

22.

Shin Y.K. and Wi C.H. (2004) Effects of temperature and salinity on survival and metabolism of the hard shelled mussel Mytilus coruscus, Bivalve; Mytilidae. Journal of Aquaculture, 17: 103-108.

23.

Shumway, S. (1977) The effects of fluctuating salinity on the tissue water content of eight species of bivalve mollusks. Journal of Comperative Physiology, 116: 269-285.

24.

Singhagraiwan T. Doi M. and Sasaki M. (1992) Salinity tolerance of juvenile donkey’s ear abalone, Haliotis asinina Linne. Thailand Marine Fisheries Reserch Bulletin. 3: 71-77.

25.

Solorzano L. (1969) Determination of ammonia in natural waters by the phenol-hypochlorite method. Limnology Oceanography, 14: 799-801.

26.

Tucker L.E. (1970) Effects of external salinity on Scutus breviculus (Gastropoda, Prosobranchia)-Ⅰ. Body weight and blood composition. Comperative Biochemistry and Physiology, 36: 301-319.

27.

Yang H.S., Park K.I., Hong C.H. and Choi K.S. (2008) Effects of salinity stress on the composition of the free amino acids of the pacific abalone Haliotis discus discus. Journal of Aquaculture, 2(4): 218-225.

28.

Yoon H.S., Rha S.J., Cha Y.B. Cho J.H., Kim K.Y. and Choi S.D. (2004) Growth and survival rate on density of Haliotis discus hannai in cage culture. Journal of Korean Fisheries Society, 37(4): 287-294.

29.

Widdows J. (1985) The effects of fluctuating and abrupt changes in salinity on the performance of Mytilus edulis. In: Gray, J. S., Christiansen, M.E. (Eds.), Marine Biology of Polar Regions and Effects of stress on marine organism. Wiley-Interscience, 555-566.

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