ISSN : 1225-3480
본 연구에서는 수온 증가에 따른 말전복 (Haliotis gigantea) 과 둥근전복 (H. discus discus) 헤모림프의 생리 및 면역학적 변화를 관찰하기 위하여 위 두 전복을 20, 22, 24, 26 및 <TEX>$28^{\circ}C$</TEX> 수온에 각각 4 일간 노출시켰다. 노출 결과, 헤모림프의 totalo-protein (TP), glucose, calcium (Ca) 은 둥근전복 (H. discus discus)이 말전복 (H. gigantea) 보다 높은 값을 보였으나, magnesium (Mg), alkaline phosphatase (ALP) 두 전복에서 유사한 값을 보였다. 그리고 면역인자인 PO에서는 둥근전복 (H. discus discus)이 더 높게 나타났다. 수온 증가에 따른 헤모림프의 TP, glucose, Mg, AST, ALT 및 ALP는 유의적인 변화가 나타나지 않았다. 하지만 헤모림프의 Ca 농도는 둥근전복 (H. discus discus)에서 <TEX>$24^{\circ}C$</TEX> 이상, 말전복 (H. gigantea)에서는 <TEX>$26^{\circ}C$</TEX> 이상의 수온에서 유의적 증가를 보였다. 그리고 면역인자인 phenoloxidase (PO) 는 둥근전복 (H. discus discus)은 <TEX>$22^{\circ}C$</TEX> 이상 말전복 (H. gigantea) 에서는 <TEX>$26^{\circ}C$</TEX> 이상의 수온에서 유의적 감소를 나타내었다. 결론적으로 말전복 (H. gigantea)은 <TEX>$24^{\circ}C$</TEX> 이상에서 혈액학적 변화를 보였고, <TEX>$26^{\circ}C$</TEX> 이상에서 면역학적 변화를 보였다. 둥근전복 (H. discus discus)은 <TEX>$26^{\circ}C$</TEX> 이상에서 혈액학적 변화를 보였고, <TEX>$22^{\circ}C$</TEX> 이상에서 면역학적 변화를 나타내었다.
This study was conducted to examine the effects of alterations in water temperature (WT) on biochemical and immunological factors in the hemolymph of the abalones, Nordotis gigantea and Haliotis discus discus. The abalone were exposed to various WT; 18, 20, 22, 24, 26 and 28℃ for 96 hours. In biochemical factors, total-protein (TP), glucose, magnesium (Mg), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were not significant changes in hemolymph of N. gigantea and H. discus. But calcium was significantly increased by high WT (≥ 24℃). In immunological factor, The phenoloxidase (PO) activity was decreased in hemolymph of N. gigantea and H.D. hannai exposed to high temperature (≥ 22℃) compared to the control (P < 0.05). Whereas alkaline phosphatase (ALP) was not significantly changed. These results suggested that high temperature adversely affects the immunity of N. gigantea and H.D. hannai.
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