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ISSN : 1225-3480
K. mikimotoi 배양체 (<TEX>$7,500cells\;ml^{-1}$</TEX>) 를 3가지 형태 (ICS, intact cell suspension; BCS, broken cell suspension; CFE, cell-free elutriate) 로 조작하여 4개 농도구배 (0, 10, 50, 100%) 로 전복치패 H. discus hannai에 노출 (온도, 13, <TEX>$18^{\circ}C$</TEX>) 하여 그 위해성을 생존율로 평가하였다. 또한, 두 적조 배양체 (C. polykrikoides와 A. affine) 를 동일형태, 농도구배 (생체량 기준), 온도에서 비교실험 하였다. 결과, K. mikimotoi는 북방전복에 어느 정도 피해를 유발하였고 (p < 0.05, 0.01), 기존 연구결과와 비교분석하였을 때, 피해기작은 세포막을 중심으로 한 독력에 의한 접촉 피해일 가능성이 있었다. 종적 비교에서, A. affine가 유의성 없이 약간 유해했다. 그러나 K. mikimotoi 독성은 strain과 성장환경에 따라 달라지며, 또한 배양체와 자연체와의 차이가 있어 본 연구결과를 일반화하기 위해서는 추가 연구가 필요한 것으로 판단되었다. 특히, 이 연구에서 실험구의 폐사시점이 대조구와 일치한다는 점에서 실험구 결과에 사육 스트레스가 부가되었음을 추측할 수 있었고, 이점은 향후 연구에서 고려할 사항으로 생각된다.
Karenia mikimotoi bloom is known to damage abalones via chemical toxicity, but the toxic mechanism remains unclear. In an attempt to gain an insight into its damage potential to abalone spats Haliotis discus hannai, the spats were exposed to 0, 10, 50, or 100% of 7,500 cells ml-1 K. mikimotoi in types of ICS (intact cell suspension), BCS (broken cell suspension) or CFE (cell-free elutriate) at 13 and 18℃, respectively. The spats were also exposed to corresponding type and dilution of 7,500 cells ml-1 Cochlodinium polykrikoides and 2,000 cells ml-1 Alexandrium affine which were equivalent to 7,500 cells ml-1 K. mikimotoi on the basis of TOC (total organic carbon). K. mikimotoi was proved to be damageable to the spats with damage potential not bigger than A. affine and C. polykrikoides. In algal type, ICS was most influential, particularly in lower dilutions (with an occasional significance of p < 0.01 or p < 0.05), suggesting that toxicity potential might be more associated with intact cell membrane. Overall, the spat damages of experimental trials were in concurrence with those of controls which were solely due to culture stress, suggesting that the culture stress might be an additional parameter influencing the spat damage and thus should be countered into consideration in future study.
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