ISSN : 1225-3480
Changes in seawater temperature that occur during the changing of the seasons are a serious challenge faced by scallop aquaculture farms. In this study, we investigated the physiological stress responses of bay scallop Argopecten irradians, to increases and decreases in water temperature. We measured the changes in the levels of expression of copper- and zinc-containing superoxide dismutase (Cu/ZnSOD), and heat shock protein (HSP) 90, as well as the concentrations of lipid peroxide (LPO) in tissues of bay scallops exposed to thermal stress conditions (low-temperature condition: 17°C → 11°C; high-temperature condition: 17°C → 25°C). The expression levels of Cu/ZnSOD and HSP90 mRNA were temperature-dependent following thermal changes. LPO concentrations increased significantly in response to temperature changes. Additionally, greater Cu/ZnSOD and HSP90 expression levels and LPO concentrations were observed under the high-temperature condition than under the low-temperature condition. The results indicate that low and high water temperature conditions induced oxidative stress in bay scallops, and that this effect was greater at high water temperatures than at low water temperatures. This study provides basic knowledge about thermal stress in bay scallops, and our results may contribute to the management of scallop aquaculture farms.
Barber, B.J. and Davis, C.V. (1997) Growth and mortality of cultured bay scallops in the Damariscotta River, Maine (USA). Aquaculture international, 5: 451-460.
Brown, H.M., Briden, A., Stokell, T., Griffin, F.J. and Cherr, G.N. (2004) Thermotolerance and HSP70profiles in adult and embryonic California native oysters, Ostreola conchaphila (Carpenter, 1857). Journal of Shellfish Research, 23: 135-141.
Côté, J., Himmelman, J.H., Claereboudt, M. and Bonardelli, J.C. (1993) Influence of density and depth on the growth of juvenile sea scallops (Placopecten magellanicus) in suspended culture. Canadian Journal of Fisheries and Aquatic Sciences, 50(9): 1857-1869.
Esterbauer, H., Schaur, R.J. and Zollner, H. (1991)Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free radical Biology and medicine, 11(1): 81-128.
Giri, S.S., Sen, S.S., Jun, J.W., Sukumaran, V. and Park, S.C. (2016) Immunotoxicological effects of cadmium on Labeo rohita, with emphasis on the expression of HSP genes. Fish and Shellfish Immunology, 54: 164-171.
Hannam, M.L., Bamber, S.D., Moody, J.A., Galloway, T.S. and Jones, M.B. (2009) Immune function in the Arctic Scallop, Chlamys islandica, following dispersed oil exposure. Aquatic toxicology, 92(3): 187-194.
Heise, K., Puntarulo, S., Pörtner, H.O. and Abele, D. (2003) Production of reactive oxygen species by isolated mitochondria of the Antarctic bivalve Laternula elliptica (King and Broderip) under heat stress. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 134(1): 79-90.
Jiang, W., Jiang, Z., Du, M., Mao, Y., Li, J., Fang, J., Lv, X., Xue, S., Wang, W., Zhang, J., Zhang, Y. and Fang, J. (2017) Physiological and biochemical responses of Zhikong scallop, Chlamys farreri, to different thermal stressors. Aquaculture Research, 48(9): 4783-4797.
Jiang, W., Li, J., Gao, Y., Mao, Y., Jiang, Z., Du, M. and Fang, J. (2016) Effects of temperature change on physiological and biochemical responses of Yesso scallop, Patinopecten yessoensis. Aquaculture, 451:463-472.
Kashiwagi, A., Kashiwagi, K., Takase, M., Hanada, H. and Nakamura, M. (1997) Comparison of catalase in diploid and haploid Rana rugosa using heat and chemical inactivation techniques. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 118(3): 499-503.
Klumpp, D.W., Humphrey, C., Huasheng, H., and Tao, F. (2002) Toxic contaminants and their biological effects in coastal waters of Xiamen, China. II. Biomarkers and embyro malformation rates as indicators of pollution stress in fish. Marine Pollution of Bulletin, 44: 761-769.
Lafrance, M., Guderley, H. and Cliché, G. (2002) Low temperature, but not air exposure slows the recuperation of juvenile scallops, Placopecten magellanicus, from exhausting escape responses. Journal of Shellfish Research, 21(2): 605-618.
Lee, J.S. and Min, D.J. (2002) A catalogue of molluscan fauna in Korea. The Korean Journal of Malacology, 18(2): 93-217.
Lin, X., Wu, X., and Liu, X. (2018) Temperature stress response of heat shock protein 90 (Hsp90) in the clam Paphia undulata. Aquaculture and fisheries, 3(3): 106-114.
Matoo, O.B., Ivanina, A.V., Ullstad, C., Beniash, E. and Sokolova, I.M. (2013) Interactive effects of elevated temperature and CO2 levels on metabolism and oxidative stress in two common marine bivalves (Crassostrea virginica and Mercenaria mercenaria). Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology, 164(4): 545-553.
Monari, M., Matozzo, V., Foschi, J., Cattani, O., Serrazanetti, G.P., and Marin, M.G. (2007) Effects of high temperatures on functional responses of haemocytes in the clam Chamelea gallina. Fish and shellfish immunology, 22(1-2): 98-114.
Pearce, C.M., Manuel, J.L., Gallager, S.M., Manning, D.A., O'Dor, R.K. and Bourget, E. (2004) Depth and timing of settlement of veligers from different populations of giant scallops, Placopecten magellanicus (Gmelin), in thermally stratified mesocosms. Journal of experimental marine biology and ecology, 312(1):187-214.
Rahman, M.A., Henderson, S., Miller-Ezzy, P., Li, X.X., and Qin, J.G. (2019) Immune response to temperature stress in three bivalve species: Pacific oyster Crassostrea gigas, Mediterranean mussel Mytilus galloprovincialis and mud cockle Katelysia rhytiphora. Fish and shellfish immunology, 86:868-874.
Song, L., Wang, L., Zhang, H. and Wang, M. (2015) The immune system and its modulation mechanism in scallop. Fish and shellfish immunology, 46(1): 65-78.
Tan, K., Zhang, B., Ma, H., Li, S., and Zheng, H. (2019)Oxidative stress responses of golden and brown noble scallops Chlamys nobilis to acute cold stress. Fish and shellfish immunology, 95: 349-356.
Zhang, H., Wang, H., Chen, H., Wang, M., Zhou, Z., Qi, L., Wang, L., Song, L. (2019) The transcriptional response of the Pacific oyster Crassostrea gigas under simultaneous bacterial and heat stresses. Developmental and Comparative Immunology, 94:1-10.
