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Simultaneous Determination of Biliary Free and Phospholipid Fatty Acids Using Gas Chromatography-Mass Spectrometry
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2000, v.13 no.5, pp.592-600
Yang,
Y. J., Lee,
S. H., &
Chung,
B.
C.
(2000). Simultaneous Determination of Biliary Free and Phospholipid Fatty Acids Using Gas Chromatography-Mass Spectrometry. Analytical Science and Technology, 13(5), 592-600.
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Abstract
The concentration of free fatty acids and fatty acid composition as well as cholesterol supersaturation in bile may be an important factor in the gallstone formation. Therefore, we simultaneously determinded 23 fatty acids in bile by selected ion monitoring (SIM) method of gas chromatography-mass spectrometry (GC-MS). Biliary fatty acids were extracted by aminopropyl column and the extracts with (phospholipid fraction) or without (free fatty acid fraction) alkaline hydrolysis of phospholipid were derivatized with MSTFA/TMCS (N-methyl-N-trimethylsilyl-trifluoroacetamide/trimethylsilylchloride) mixture in order to be detected on the GC-MS. The recovery range of this method was 61.1-99.0% and the RSD value of within-a-day and day-to-day test were 3.1-25.6% and 3.8-27.0%, respectively. Using this method, biliary profile was investigated in the bile of normal controls and patients with gallstones. The amounts and their distribution of free and phospholipid fatty acids showed different pattern between normal subjects and patients.
- keywords
- fatty acid, gas chromatography-mass spectrometry, biliary profile
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