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Homogeneous Enzyme-linked Binding Assay for Mistletoe Lectin I Based on the Mistletoe Lectin I/D-galactose Interaction
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2000, v.13 no.5, pp.624-629
Rhee Paeng,
I., Lee,
E. A., &
Chon,
C.
S.
(2000). Homogeneous Enzyme-linked Binding Assay for Mistletoe Lectin I Based on the Mistletoe Lectin I/D-galactose Interaction. Analytical Science and Technology, 13(5), 624-629.
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Abstract
A simple and rapid homogeneous enzyme-linked binding assay for mistletoe lectin I(ML I) was developed using a coupled enzyme system of malate dehydrogenase (MDH) and D-galactose. A highly substituted MDH-galactose conjugate was prepared by employing an isothiocyanate method for formation of thiourea bond. In the presence of ML I, ML I inhibits the activity of the conjugate based on the ML I/D-galactose specific interaction. Thus, the concentration of ML I can be related to the homogeneous inhibition of the MDH-galactose conjugate. Using this method. ML I can be measured at the level of microgram per milliliter within 10 minutes.
- keywords
- mistletoe lectin I, D-galactose, homogeneous assay, enzyme-linked binding assay
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