- P-ISSN 1225-0163
- E-ISSN 2288-8985
In this study, we determined distribution status of organonitrogen pesticides such as trifluralin, atrazine, metribuzin, alachlor, malathion, nitrofen, permethrin, cypermethrin, fenvalerate, and parathion accumulated in the fresh water fishes and amphibians. We collected those samples at 31 sites from the basin of major rivers, and separated the muscular tissue as a final sample for analysis. In the pretreatment process, lipid was eliminated by using acetonitrile and n-hexane, and pesticides were reextracted with dichloromethane. The extract was dehydrated and concentrated, and then cleaned it up by passing the Florisil column, and pesticide content was finally determined by using a GC-MS system after introducing isotope labelled references. The accumulation level was observed in the range of <TEX>$0.17{\sim}6.8{\mu}g/kg$</TEX> in amphibians and <TEX>$0.26{\sim}16{\mu}g/kg$</TEX> in fishes including cypermethrin as <TEX>$16{\mu}g/kg$</TEX>.