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Determination of residual novobiocin in livestock products and fisheries products by HPLC
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2007, v.20 no.4, pp.347-354
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(2007). Determination of residual novobiocin in livestock products and fisheries products by HPLC. Analytical Science and Technology, 20(4), 347-354.
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Abstract
A simple and rapid high-performance liquid chromatography assay for the determination of residual novobiocin levels in bovine, porcine, chicken, flatfish and japanese eel muscle has been developed and validated. The separation condition for HPLC/UV was optimized with phenyl hexyl (<TEX>$4.6{\times}150mm$</TEX>, <TEX>$5{\mu}m$</TEX>) column with 10 mM monobasic sodium phosphate buffer (pH 2.5)/acetonitrile (50/50, v/v) as the mobile phase at a flow rate of 1.0 mL/min and detection wavelength was set at 254 nm. Residues were extracted from tissue by blending with methanol and lipid materials were removed with n-hexane. Then, the methanol extract was evaporated to dryness under a nitrogen stream, reconstituted in the mobile phase. Aliquot of the organic extract was decanted and filtered through <TEX>$0.45{\mu}m$</TEX> syringe filter. The <TEX>$20{\mu}L$</TEX> of the resulting solution was injected into the HPLC system. The calibration ranges were <TEX>$0.5{\sim}5{\mu}g/g$</TEX> and calibration curves were linear with coefficients of correlation better than 0.95. The limits of quantification were <TEX>$0.5{\mu}g/g$</TEX> for all muscles. The recoveries of bovine, porcine, chicken, flatfish and japaneseel muscles were 99.8%, 102.4%, 91.0%, 104.0% and 93.0%, respectively. The procedures were validated according to the CODEX guideline, determining specificity, linearity, accuracy, precision, quantitation limit and recovery.
- keywords
- Novobiocin, residue, livestock products, fisheries products, HPLC/UV
Reference
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