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Simultaneous analysis of ethylene glycol and glycolic acid in bio-specimens by GC/MS
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2010, v.23 no.6, pp.544-550
https://doi.org/10.5806/AST.2010.23.6.544
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(2010). Simultaneous analysis of ethylene glycol and glycolic acid in bio-specimens by GC/MS. Analytical Science and Technology, 23(6), 544-550, https://doi.org/10.5806/AST.2010.23.6.544
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Abstract
Mistaking pink colored thermal oil for grape wine, a victim drank the oil to death which was analyzed to contain 39% of ethylene glycol. Thermal oil could be used for heat transfer to prevent the malfunction due to the high pressure in the boiler operated at high temperature when using water. Main component of thermal oil is known to be mineral oil or ethylene glycol. From the blood and other tissue of the victim from autopsy,ethylene glycol and its metabolite were simultaneously analyzed by GC/MS after extraction under acidic condition with acetonitrile followed by derivatization with BSTFA. About 0.2 g of the specimens were pretreated with 50 uL of 0.5 M HCl solution to keep acidic condition, then dehydrated with anhydrous sodium sulfate followed by concentration under nitrogen stream. Ethylene glycol and glycolic acid concentration in blood was measured to be 2,755 μg/mL and 174 μg/mL respectively. In other specimen, the concentration of ethylene glycol and glycolic acid was 860 μg/g~1,290 μg/g and 93 μg/g~134 μg/g. Especially, crystal appeared in kidney which was supposed xalate from the metabolite of ethylene glycol.
- keywords
- ethylene glycol, glycolic acid, ethylene glycol-<TEX>$d_4$</TEX>, derivatization, GC/MS
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