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- E-ISSN 2288-8985
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Development of intracellular organelle markers using modified glycolipid-binding peptides in mammalian cells
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2015, v.28 no.1, pp.65-71
https://doi.org/10.5806/AST.2015.28.1.65
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(2015). Development of intracellular organelle markers using modified glycolipid-binding peptides in mammalian cells. Analytical Science and Technology, 28(1), 65-71, https://doi.org/10.5806/AST.2015.28.1.65
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Abstract
Intracellular organelles in eukaryotic cells play important roles in many cellular functions. Intracellulartrafficking of many proteins to specific intracellular organelles is tightly regulated by various mechanisms in cells. Therefore, elucidating the targeting mechanism of novel markers for intracellular organelles is important for cellularphysiology and pathology. In this study, we tried to identify the peptides which could bind to specific glycolipidin cellular membrane using GFP-fused glycolipid-binding peptides, and analyzed their cellular localization. As aresult, we could identify mitochondria-, Golgi- or plasma membrane-targeting peptides. Furthermore, we found thatthe plasma membrane-targeting peptide was localized to the plasma membrane via electrostatic interactions. Thus,our results suggest that various glycolipid-binding peptides could be used as intracellular organelles markers.
- keywords
- glycolipid-binding peptide, intracellular organelles, marker, plasma membrane, electrostatic interaction
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