- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Log In/Sign Up
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Simultaneous determination of 11-nor-Δ9-carboxy-tetrahydrocannabinol and 11-nor-Δ9-carboxy-tetrahydrocannabinol-glucuronide in urine samples by LC-MS/MS and its application to forensic science
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2021, v.34 no.6, pp.259-266
https://doi.org/10.5806/AST.2021.34.6.259
&
(2021). Simultaneous determination of 11-nor-Δ9-carboxy-tetrahydrocannabinol and 11-nor-Δ9-carboxy-tetrahydrocannabinol-glucuronide in urine samples by LC-MS/MS and its application to forensic science. Analytical Science and Technology, 34(6), 259-266, https://doi.org/10.5806/AST.2021.34.6.259
- Downloaded
- Viewed
Abstract
Cannabis (Marijuana) is one of the most widely used drugs in the world, and its distribution has been controlled in South Korea since 1976. Identification of 11-nor-Δ9-carboxy-tetrahydrocannabinol (THCCOOH) in urine can provide important proof of cannabis use, and it is considered scientific evidence in the forensic field. In this study, we describe a simultaneous quantitative method for identifying THCCOOH and THCCOOHglucuronide in urine, using simple liquid–liquid extraction (LLE), and liquid chromatography-tandem mass spectrometry (LC-MS/MS). THCCOOH-D3 and THCCOOH-glucuronide-D3 were used as internal standards. Validation results of the matrix effect, as well as recovery, linearity, precision, accuracy, process efficiency, and stability were all satisfactory. No carryover, endogenous or exogenous interferences were observed. The limit of detection (LOD) of THCCOOH and THCCOOH-glucuronide were 0.3 and 0.2 ng/mL, respectively. The developed method was applied to 28 authentic human urine samples that tested positive in immunoassay screening and gas chromatography/mass spectrometry (GC/MS) tests. The ranges of concentrations of THCCOOH and THCCOOH-glucuronide in the samples were less than LOQ~266.90 ng/mL and 6.43~2133.03 ng/mL, respectively. The concentrations of THCCOOH-glucuronide were higher than those of THCCOOH in all samples. This method can be effectively and successfully applied for the confirmation of cannabinoid use in human urine samples in the forensic field.
- keywords
- forensic, cannabinoid glucuronide, LC-MS/MS, LLE
Reference
1
1. H. S. Chung, ‘Drug testing in biological fluids’, Shinilbooks, Seoul, 2000.
2
2. ‘Narcotics Monthly Trends’, Supreme Prosecutor’s Office, 2020.
3
3. R. S. Goodwin, W. D. Darwin, C. N. Chiang, M, Shih, S. H. Li and M. A. Huestis, J. Anal. Toxicol., 32(8), 562-569 (2008).
4
4. F. Musshoff and B. Madea, Ther. Drug Monit., 28, 155-163 (2006).
5
5. C. Baselt and R. H. Cravey, ‘Disposition of Toxic Drugs and Chemicals in Man’, 11th ed., Chemical toxicology institute, Forster city, California, 2017.
6
6. M. E. Wall, B. M. Sadler, D. Brine, H. Taylor and M. Perez-Reyes, Clin. Pharmacol. Ther., 34, 352-363 (1983).
7
7. U. Mareck, N. Haenelt, H. Geyer, S. Guddat, M. Kamber, R. Brenneisen, M. Thevis and W. Schänzer, Drug Test. Anal., 1, 505-510 (2009).
8
8. ‘Recommended Methods for the Detection and assay of Heroin, Cannabinoids, cocaine, Amphetamine, Methamphetamine and Ring-substitute amphetamine derivatives in Biological Specimens: Manual for use by National Laboratories’, United Nations Office on Drugs and Crime(UNODC), Vienna, 1995.
9
9. ‘Urine Testing for Drugs of Abuse’, National Institute on Drug Abuse(NIDA) Research Monograph 73, Baltimore, 1980.
10
10. T. T. Abraham, R. H. Lowe, S. O. Pimay, W. D. Darwin and M. A. Huestis, J. Anal. Toxicol., 31(8), 477-485 (2007).
11
11. ‘The fitness for purpose of analytical methods-A laboratory guide to method validation and related topics’, EURACHEM, Oly(UK), 1998.
12
12. F. T. Peter, O. H. Drummer and F. Musshoff, Forensic Sci. Int., 165, 216-224 (2007).
13
13. B. K. Matuszewski, M. L. Constanzer and C. M. Chavez-Eng, Anal. Chem., 75, 3019-3030 (2003).
14
14. S. D. Ferrara, L. Tedeschi and G. Frison, J. Anal. Toxicol., 18, 278-291 (1994).
15
15. Z. J. Nelson, S. J. Stellpfug and K. M. Engebrestsen, J. Pharm. Pract., 29(5), 516-526 (2016).
16
16. O. Boldis, G. Kocsis, A. Gachalyi and J. Furesz, J. Chromatogr. Sci., 41(4), 190-194 (2003).
17
17. W. H. Abd-Elsalam, M. A. Asherbiny, J. Y. Kung, D. W. Pate and R. Lobenberg, Talanta, 204, 846-967 (2019).
18
18. M. J. Rumpler, J. Chromatogr. B., 957, 77-83 (2014).
19
19. M. J. Park, J. H. Kim, Y. R. Park, S. H. In, E. M. Kim and Y. H. Park, J. Chromatogr. B., 947-948, 179-185 (2014).
20
20. M. H. Jang, I. C. Shin, W. K. Yang, H. J. Chang, H. Y. Yoo, J. S. Lee and E. M. Kim, Forensic Sci. Int., 244, 85-91 (2014).
Other articles from this issue
- Regression model for the preparation of calibration curve in the quantitative LC-MS/MS analysis of urinary methamphetamine, amphetamine and 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid using R
- A study on the distribution of latent fingerprints on paper knife sheaths
- Simultaneous determination of 11-nor-Δ9-carboxy-tetrahydrocannabinol and 11-nor-Δ9-carboxy-tetrahydrocannabinol-glucuronide in urine samples by LC-MS/MS and its application to forensic science
- Validation of acid-base titration equivalence between spectroscopic titrator (SPT) and conventional titrators
- Fabrication and application of cell-based microfluidic chip for eye-irritation test of chemicals
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.