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Rapid Separation and Indentification Method of Tea Catechins
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1992, v.5 no.3, pp.333-338
Lee,
J., Lee,
Y., &
Moon,
D.
(1992). Rapid Separation and Indentification Method of Tea Catechins. Analytical Science and Technology, 5(3), 333-338.
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Abstract
The tea tannins, epigallocatechin, epigallocatechin gallate, were successfully separated by a Sephadex LH-20 column by the acetone based gradient elution. Each fractions was collected by monitoring at 280nm. Purified fractions were directly characterized by fast atom bombardment mass spectrometry. Epigallocatechin and epigallocatechin gallate were identified and shown as low as 70% purity in the reversed phase column. This revised method is more advantageous than known methods in purity and rapidity.
- keywords
- tannins, catechins, separation, FAB-MS, HPLC
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