- Apply for Authority
- KOREAN
- P-ISSN2287-8327
- E-ISSN2288-1220
- SCOPUS, KCI
ISSN : 2287-8327
Growth characteristics and lipid content of three Korean isolates of Botryococcus braunii (Trebouxiophyceae)
(School of Life Sciences, Kyungpook National University)
(Lotte Chemical Research Institute)
- Downloaded
- Viewed
Abstract
Three strains of the green microalga Botryococcus braunii (JJS, KCM, and KJD) were isolated from different water bodies in Korea and grown as batch cultures in the laboratory. The effects of different growth media and temperatures on the growth rate were investigated, as well as the effect of temperature on the total lipid content and lipid profile. All three strains had the highest growth rates in BG-11 medium and at 25°C. Maximal lipid production (g L-1) was at 30°C in the JJS strain and at 25°C in the KCM and KJD strains. However, all the three strains produced the greatest percent dry weight of total lipids at 15°C and had the lowest percent dry weight of total lipids at 25°C. In general, oleic acid, linolenic acid, and behenic acid were the most common fatty acids in all three strains. However, the three strains varied considerably in their fatty acid profiles at different culture temperatures.
- keywords
- Botryococcus braunii, growth rate, lipid content, media, temperatures
Reference
Abou-Shanab RAI, Hwang JH, Cho Y, Min B, Jeon BH. 2011. Characterization of microalgal species isolated from fresh water bodies as a potential source for biodiesel production. Appl Energy 88: 3300-3306.
Ashokkumar V, Rengasamy R. 2012. Mass culture of Botryococcus braunii Kutz. under open raceway pond for biofuel production. Bioresour Technol 104: 394-399.
Bligh EG, Dyer WJ. 1959. A rapid method for total lipid extraction and purification. Can J Biochem Physiol 37: 911-917.
Casadevall E, Dif D, Largeau C, Gudin C, Chaumont D, Desanti O. 1985. Studies on batch and continuous cultures of Botryococcus braunii: hydrocarbon production in relation to physiological state, cell ultrastructure, and phosphate nutrition. Biotechnol Bioeng 27: 286-295.
Chisti Y. 2007. Biodiesel from microalgae. Biotechnol Adv 25: 294-306.
Choi GG, Kim BH, Ahn CY, Oh HM. 2011. Effect of nitrogen limitation on oleic acid biosynthesis in Botryococcus braunii. J Appl Phycol 23: 1031-1037.
Dayananda C, Sarada R, Bhattacharya S, Ravishankar GA. 2005. Effect of media and culture conditions on growth and hydrocarbon production by Botryococcus braunii. Process Biochem 40: 3125-3131.
Dayananda C, Sarada R, Rani MU, Shamala TR, Ravishankar GA. 2007. Autotrophic cultivation of Botryococcus braunii for the production of hydrocarbons and exopolysaccharides in various media. Biomass Bioenerg 31: 87-93.
Gallagher JC. 1982. Physiological variation and electrophoretic banding patterns of genetically different seasonal populations of Skeletonema costatum (Bacillariophyceae). J Phycol 18: 148-162.
Gallagher JC. 1986. Population genetics of microalgae. Nova Hedwigia Beih 83: 6-14.
Ge Y, Liu J, Tian G. 2011. Growth characteristics of Botryococcus braunii 765 under high CO2 concentration in photobioreactor. Bioresour Technol 102: 130-134.
Hillen LW, Pollard G, Wake LV, White N. 1982. Hydrocracking of the oils of Botryococcus braunii to transport fuels. Biotechnol Bioeng 24: 193-205.
Inoue H, Korenaga T, Sagami H, Koyama T, Sugiyama H, Ogura K. 1994. Formation of farnesyl oleate and 3 other farnesyl fatty-acid esters by cell-free-extracts from Botryococcus-braunii-B race. Phytochemistry 36: 1203-1207.
Kalacheva GS, Zhila NO, Volova TG, Gladyshev MI. 2002. The effect of temperature on the lipid composition of the green alga Botryococcus. Microbiology 71: 286-293.
Kim BH, Ramanan R, Cho DH, Choi GG, La HJ, Ahn CY, Oh HM, Kim HS. 2012. Simple, rapid and cost-effective method for high quality nucleic acids extraction from different strains of Botryococcus braunii. PLoS ONE 7: e37770.
Kim JH, Lee KL, Kim HS. 2009. Effect of nutrients and light intensity on growth of Mallomonas caudate (Synurophyceae). Nord J Bot 27: 516-522.
Knothe G. 2008. “Designer” biodiesel: optimizing fatty ester composition to improve fuel properties. Energy Fuels 22: 1358-1364.
Largeau C, Casadevall E, Berkaloff C. 1980. The biosynthesis of long-chain hydrocarbons in the green alga Botryococcus braunii. Phytochemistry 19: 1081-1085.
Lee KL, Kim HS. 2007. Growth characteristics of three synurophytes (Mallomonas species) at different temperatures and pH. Nova Hedwigia 84: 227-240.
Lehman JT. 1976. Ecological and nutritional studies on Dinobryon Ehrenb: seasonal periodicity and the phosphate toxicity problem. Limnol Oceanogr 21: 646-658.
Levasseur M, Thompson PA, Harrison PJ. 1993. Physiological acclimation of marine phytoplankton to different nitrogen sources. J Phycol 29: 587-595.
Li Y, Qin JG. 2005. Comparison of growth and lipid content in three Botryococcus braunii strains. J Appl Phycol 17: 551-556.
Lupi FM, Fernandes HML, Sá-Correia I, Novais JM. 1991. Temperature profiles of cellular growth and exopolysaccharide synthesis by Botryococcus braunii Kütz. UC 58. J Appl Phycol 3: 35-42.
Mata TM, Martins AA, Caetano NS. 2010. Microalgae for biodiesel production and other applications: a review. Renew Sustainable Energy Rev 14: 217-232.
Metzger P, Largeau C. 2005. Botryococcus braunii: a rich source for hydrocarbons and related ether lipids. Appl Microbiol Biotechnol 66: 486-496.
Oh HM, Yoo C, Lee JY, Ahn C. 2009. Physiological study on the increased lipid production of Botryococcus Braunii. Phycologia 48: 98-98.
Ranga Rao A, Sarada R, Ravishankar GA. 2007. Influence of CO2 on growth and hydrocarbon production in Botryococcus braunii. J Microbiol Biotechnol 17: 414-419.
Sawayama S, Inoue S, Yokoyama S. 1994. Continuous culture of hydrocarbon-rich microalga Botryococcus braunii in secondarily treated sewage. Appl Microbiol Biotechnol 41: 729-731.
Soudek DJ, Robinson GGC. 1983. Electrophoretic analysis of the species and population structure of the diatom Asterionella formosa. Can J Bot 61: 418-433.
Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G. 1971. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev 35: 171-205.
Sushchik NN, Kalacheva GS, Zhila NO, Gladyshev MI, Volova TG. 2003. A temperature dependence of the intra- and extracellular fatty-acid composition of green algae and Cyanobacterium. Russ J Plant Physiol 50: 374-380.
Wake LV, Hillen LW. 1980. Study of a “bloom” of the oil-rich alga Botryococcus braunii in the Darwin River Reservoir. Biotechnol Bioeng 22: 1637-1656.
Xu N, Zhang X, Fan X, Han L, Zeng C. 2001. Effects of nitrogen source and concentration on growth rate and fatty acid composition of Ellipsoidion sp. (Eustigmatophyta). J Appl Phycol 13: 463-469.
Yeesang C, Cheirsilp B. 2011. Effect of nitrogen, salt, and iron content in the growth medium and light intensity on lipid production by microalgae isolated from freshwater sources in Thailand. Bioresour Technol 102: 3034-3040.
Zhila NO, Kalacheva GS, Volova TG. 2005a. Effect of nitrogen limitation on the growth and lipid composition of the green alga Botryococcus braunii Kutz IPPAS H-252. Russ J Plant Physiol 52: 311-319.
Zhila NO, Kalacheva GS, Volova TG. 2005b. Influence of nitrogen deficiency on biochemical composition of the green alga Botryococcus. J Appl Phycol 17: 309-315.
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations