Cultural contents should provide all the people web services to ensure accessibility without any obstacles as a core resource of nation including a code linking people and people beyond time and space. This study achieved that automated measure including 11 checkpoints and manual analysis including 11 criteria and 26 checkpoints of three areas in selected five web sites such as Culture Portal. Art Road, Korean Patterns, MFM, and e-Museum supported by Ministry of Culture & Tourism. As a result, web accessibility level of cultural contents analyzed it staying at the beginning level. This study Provided the directions in order to improve accessibility of cultural contents through showing real cases to practice for improving web accessibility.