ISSN : 1225-3480
With the purpose to estimate the possibility of short-term storage and cryopreservation for sperm of Charonia sauliae, which is a potential preparation for its artificial reproduction and further research, in this study, protocols for short-term storage and cryopreservation of trumpet shell sperm was optimized. The effects of different immobilizing solutions, dilution ratios were estimated for short-term storage. And the effects of different cryoprotectant extenders and freezing rates were estimated for cryopreservation in terms of motility and survival of sperm. The results indicated that the artificial sea water of 350 mOsmol/kg is a better immobilizing solution and sperm which was diluted at a ratio of 1:1 (v/v) had higher motility and survival rate during short-term storage. The effect of 5% dimethyl sulfoxide was significantly better than those of other cryoprotectant extenders. And a freezing rate of -20℃ min-1 showed better effect than other freezing rates. In conclusion, this study optimized some key factors of the short-term and cryopreservation of C. sauliae sperm, which can provide valuable data for germplasm conservation and artificial propagation of C. sauliae.
Acosta-Salmóna, H, (2007) Effects of cryoprotectant agents and freezing protocol on motility of black-lip pearl oyster (Pinctada margaritifera L.) spermatozoa, Cryobiology
Adams, S.L, (2004) Cryopreservation of sperm of the Pacific oyster (Crassostrea gigas): development of a practical method for commercial spat production, Aquaculture
Blaxer,J.H.S, (1953) Sperm storage and cross-fertilization of spring and autumn spawning herring, Nature
Bllom,E, (1950) A one-minute live-dead sperm stain by means of eosin-nigrosin, Fertility and Sterility
Dong, Q, (2005) Systematic factor optimization for cryopreservation of shipped sperm samples of diploid Pacific oysters, Crassostrea gigas, Cryobiology
Fabbrocini, A, (2000) Cryopreservation of seabream (Sparus aurata) spermatozoa, Cryobiology
Fahning, M.L, (1992) Status of Cryopreservation of embryos from domestic animals, Cryobiology
Fribourgh,J.H, (1966) The application of a differential staining method to low-temperature studies on goldfish spermatozoa, Progressive Fish-Culturist
Gwo,J.C, (1994) Cryopreservation of yellowfin seabream (Acanthopagrus latus) spermatozoa (teleost, perciformes, sparides), Theriogenology
Kang, K.H, (2004) The predation of trumpet shell, Charonia sp., on eight different marine invertebrate species, Aquaculture Research
Lahnsteiner, F, (1996) The influence of various cryoprotectants on semen quality of the rainbow trout (O. mykiss) before and after cryopreservation, Journal of Applied Ichthyology
Liu, Q.H, (2007) Flow cytometry and ultrastructure of cryopreserved red seabream(Pagrus major) sperm, Theriogenology
Mazur,P, (1984) Freezing of living cells -- Mechanisms and implications, American Journal of Physiology
Morisawa, M, (1990) Acquisition and initiation of sperm motility. In: Controls of Sperm Motility: Biological and Clinical Aspects (ed. by Gagnon, C.), CRC Press, London
Nascimento, I.A, (2005) Selection of cryoprotectants based on their toxic effects on oyster gametes and embryos, Cryobiology
Renard, P, (1989) Effect of various cryoprotectants on pacific oyster Crassostrea gigas Thunberg, manila clam Ruditapes philippinarum Reeve and scallop Pecten maximus (L.) embryos: influence of the biochemical and osmotic effects, Cryoletters
Ritar, A.J, (2000) Sperm survival during short-term storage and after cryopreservation of semen from striped trumpeter (Latris lineata), Theriogenology
Smith, J, (2001) Cryopreservation of shellfish sperm, eggs and embryos, Proceedings of the New Zealand Society of Animal Production
Suquet, M, (2000) Cryopreservation of sperm in marine fish, Aquaculture Research
Usuki, H, (2002) Effects of developmental stage, seawater concentration and rearing temperature on cryopreservation of Pacific oyster Crassostrea gigas larvae, Fisheries Science
Yao, Z, (2000) Motility, fertility and ultrastructural changes of ocean pout (Macrozoarces americanus L.) sperm after cryopreservation, Aquaculture
