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ISSN : 1225-3480
본 연구는 카드뮴 노출에 따른 북방전복, Haliotis discus hannai의 hemolymph 및 hepatopancreas의 효소활성에 대한 변화를 관찰하였다. 북방전복은 카드뮴 0, 5, 10, 20 및 40 Cd μg/L의 농도에서 4 주간 노출시킨 결과, hemolymph의phenoloxidase (PO) 활성은 40 Cd μg/L 농도에서 4주 후에 유의하게 감소하였으나, alkaline phosphatase (ALP), aspartate aminotransferase (AST) 및 alanine aminotransferase (ALT) 활성은 40 Cd μg/L 농도에서 4 주 후에 유의한 증가를 나타냈다. 또한 hemolymph의 칼슘(Ca) 농도는 20 및 40 Cd μg/L의 농도에서 4주 후에 유의한감소를 나타냈다. 한편 hepatopancreas의 superoxide dismutase (SOD) 활성은 20 및 40 Cd μg/L 농도에서, catalase (CAT) 활성은 40 Cd μg/L 농도에서 2주 후에 유의한 증가를 나타냈다. 따라서 카드뮴에 노출된 북방전복hemolymph와 hepatopancreas의 효소활성은 적어도 카드뮴농도 40 Cd μg/L에서 변동이 있을 것으로 예상되며, 이들 효소활성은 연안의 중금속 오염 모니터링의 기초 자료로 활용할수 있을 것으로 생각된다.
This study was conducted to investigate the effects of cadmium (Cd) exposure on biochemical factors in the hemolymph and hepatopancreas of the abalone, Haliotis discus hannai. The abalone were exposed to 0, 5, 10, 20 and 40 μg/L Cd for 4 weeks. The phenoloxidase (PO) activity was decreased in hemolymph of abalone exposed to 40 Cd μg/L for 4 weeks compared to the control (P < 0.05). The hemolymph enzymes, alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were markedly elevated in 40 Cd μg/L after 4 weeks. The hemolymph calcium concentrations were significantly decreased in 20 and 40 Cd μg/L for 4 weeks. Hepatopancreas superoxide dismutase (SOD) and catalase (CAT) activities were significantly increased by Cd. SOD was increased in both 20 and 40 Cd μg/L and CAT, in 40 Cd μg/L after 2 weeks (P < 0.05). These results suggested that the abalone SOD and CAT including PO may serve as a protective mechanism against oxidative stress by Cd. We conclude that a Cd concentration, 40 μg/L in water may curtail hemolymph homeostasis and anti-oxidative reactions in abalone hepatopancreas. From these results, these biochemical factors may represent a convenient method of monitoring heavy metal pollution in coastal areas.
Abdel-Moati, M.A.R. (1992) Biochemical and physiological responses of Mytilus edulis to Hg and Pb in the coastal waters of Alexandria region. Proceeding of the FAD-UNEP-IOC workshop on biological effects of pollutants in marine organisms. Athens, Greece, 69:61-72.
Abel, T.H. and Bärlocher, F. (1984) Effect of cadmium on aquatic hyphomycetes. Applied and Environmental Microbiology, 48: 245-251.
Barton, B.A. (1991) Physiological changes in fish from stress in aquaculture with emphasis in the response and effects of corticosteroids. Annual Review of Fish diseases, 1: 3-26.
Bhattacharyya, M.H., Whelton, B.D., Stern, P.H. and Peterson, D.P. (1988) Cadmium accelerates bone loss in ovariectomized mice and fetal rat limb bones in culture. Proceedings of the National Academy of Sciences of the United States of America, 85: 8761–8765.
Blasco, J. and Puppo, J. (1999) Effect of heavy metals (Cu, Cd and Pb) on aspartate and alanine aminotransferase in Ruditapes philippinarum (Mollusca: Bivalvia). Comparative biochemistry and physiology Part C: Pharmacology, toxicology &endocrinology, 122: 253-263.
Bogé, G., Bussiere, D. and Pérès, G. (1988) Effets du chrome hexavalent sur les fonctions de la bordure en brosse de l'intestin de la truite arc en ciel (Salmo gairdneri R.). Water Research, 22: 441-447.
Cheng, T.C. (1981) Bivalves. In: Invertebrate blood cell (ed. by Ratcliffe, N.A., Rowley, A.F.), London Academic Press, London. pp. 233-299.
Cong, R., Sun, W., Liu, G., Fan, T., Meng, X., Yang, L. and Zhu, L. (2005) Purification and characterization of phenoloxidase form clam Ruditapes philippinarum. Fish and Shellfish Immunology, 18: 61-70.
Courtois, E., Marques, M., Barrientos, A., Casado, S. and López-Farré, A. (2003) Lead-induced downregulation of soluble guanylate cyclase in isolated rat aortic segments mediated by reactive oxygen species and cyclooxygenase-2. Journal of the American Society of Nephrology, 14: 1464–1470.
Ferraris, M., Radice, S., Catalani, P., Francolini, M., Marabini, L. and Chiesara, E. (2002) Early oxidative damage in primary cultured trout hepatocytes: a time course study. Aquatic Toxicology, 59: 283-296.
Firat, Ö., Çogun, H.Y., Aslanyavrusu, S. and Kargin, F. (2009) Antioxidant responses and metal accumulation in tissues of Nile tilapia Oreochromis niloticus under Zn, Cd and Zn + Cd exposure. Journal of Applied Toxicology, 29: 295-301.
Forbes, V.E. and Depledge, M.H. (1992) Cadmium effects on the carbon and energy balance of mudsnails. Marine Biology, 113: 263-269.
Fried, G.H. and Levin, N.L. (1973) Enzymatic activity in hepatopancreas of Nassarius obsoletus. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 45: 153-157.
Halliwell, B. and Gutteridge, J.M. (1995) The definition and measurement of antioxidants in biological systems. Free Radical Biology and Medicine, 18:125-126.
Hontela, A., Daniel, C. and Ricard, A.C. (1996) Effects of acute and subacute exposures to cadmium on the interrenal and thyroid function in rainbow trout, Oncorhynchus mykiss. Aquatic Toxicology, 35:171-182.
Hu, G.F., Li, Z., Liang, H.W., Wang, C.Z., Wu, Q.C., Luo, X.Z. and Zou, G.W. (2009) Effects of cadmium on SOD and CAT in hepatopancreas, antennary gland and gill of Procambarus clarkii. Journal of Agro-Environment Science, 28: 1806-1811.
Huang, X., Ke, C. and Wang, W.X. (2008)Bioaccumulation of silver, cadmium and mercury in the abalone Haliotis diversicolor from water and food source. Aquaculture, 283: 194-202.
Huang, X., Guo, F., Ke, C. and Wang, W.X. (2010)Response of abalone Haliotis diversicolor to sublethal exposure of waterborne and dietary silver and cadmium. Ecotoxicology and Environmental Safety, 73: 1130-1137.
Hur, J.W., Chang, Y.J., Lim, H.K. and Lee, B.K. (2001)Stress responses of cultured fishes elicited by water level reduction in rearing tank and fish transference during selection process. Bulletin of the Korean Fisheries Society, 34: 465-472.
Hwang, U.G., Kim, J.H. and Kang, J.C. (2013) Changes of growth and hematological constituents in the rock bream Oplegnathus fasciatus exposed to TBT. Journal Fish Pathology, 26: 219-229.
Jee, J.H., Kim, S.G. and Kang, J.C. (2004) Effects of phenanthrene on growth and basic physiological functions of the olive flounder, Paralichthys olivaceus. Journal of Experimental Marine Biology and Ecology, 304: 123-136.
Johnson, J.K., Rocheleau, T.A., Hillyer, J.F., Chen, C.C., Li, J. and Christensen, B.M. (2003) A potential role for phenylalanine hydroxylase in mosquito immune responses. Insect Biochemistry and Molecular Biology, 33: 345-354.
Kim, T.H., Yang, M.H., Choe, M.K., Han, S.J. and Yeu, I.K. (2005) Physiological studies on acute water-temperature stress of juvenile abalone, Haliotis discus hannai. Journal of Aquaculture, 18: 7-12.
Kim, T.H., Kim, K.J., Choe, M.K. and Yeo, I.K. (2006)Physiological changes of juvenile abalone, Haliotis sieboldii exposed to acute water temperature stress. Journal of Aquaculture, 19: 77-83.
KNSO (2011) Korea National Statistical office. Fishery production survey DB, Daejeon, Korea.
Koyama, J. and Itazawa, Y. (1977) Effects of oral administration of cadmium on fish: I. Analytical results of the blood and bones. Bulletin of the Japanese Society of Scientific Fishries, 43: 523-526.
Liu, H., Zhang, J.F., Shen, H., Wang, X.R. and Wang, W.M. (2005) Impact of copper and Its EDTA complex on the glutathione-dependent antioxidant system in freshwater fish (Carassius auratus). Bulletin of Environmental Contamination and Toxicology, 74:1111–1117.
Lopes, P.A., Pinheiro, T., Santos, M.C., da Luz Mathias, M., Collares-Pereira, M.J. and Viegas-Crespo, A.M. (2001) Response of antioxidant enzymes in freshwater fish population (Leuciscus alburnoides complex) to inorganic pollutants exposure. Science of the Total Environment, 280: 153-163.
Mok, J.S., Lee, K.J., Shim, K.B., Lee, T.S., Song, K.C. and Kim, J.H. (2010) Contents of heavy metals in marine invertebrates from the Korean coast. Journal of the Korean Society of Food Science and Nutrition, 39: 894-901.
Moody, C.S. and Hassan, H.M. (1982) Mutagenicity of oxygen free radicals. Proceedings of the National Academy Sciences, 79: 2855-2859.
Nebot, C., Moutet, M., Huet, P., Xu, J.Z., Yadan, J.C. and Chaudiere, J. (1993) Spectrophotometric assay of superoxide dismutase activity based on the activated autoxidation of a tetracyclic catechol. Analytical Biochemistry, 214: 442-451.
Parihar, M.S., Javeri, T., Hemnani, T., Dubey, A.K. and Parkash, P. (1997) Responses of superoxide dismutase, glutathione peroxidase and reduced glutathione antioxidant defenses in gills of the freshwater catfish (Heteropneustes fossilis) to short-term elevated temperature. Journal of Thermal Biology, 22: 151-156.
Park, C.J., Min, B.H., Kim, K.S., Lee, J.W., Lee, J.H., Noh, J.K., Kim, H.C., Park, J.W. and Myeong, J.I. (2011) Physiological responses on low water temperature stress of Pacific abalone, Haliotis discus hannai. Korean Journal of Malacology, 27: 317-322.
Park, H.J. and Kang, J.C. (2012) Biochemical changes in the hemolymph and hepatopancreas of abalone Haliotis discus hannai exposed to copper. Korean Journal of Fisheries and Aquatic Sciences, 45:154-160.
Pipe, R.K., Coles, J.A., Carissan, F.M.M. and Ramanathan, K. (1999) Copper induced immunomodulation in the marine mussel, Mytilus edulis. Aquatic Toxicology, 46: 43-54.
Shen, H., Zhang, Q., Xu, R. and Wang, G. (1997) Effects of petroleum on the sero-proteins of Tilapia mossambica. Marine Environmental Science, 16: 1-5.
Shin, Y.K., Jun, J.C., Im, J.H., Kim, D.W., Son, M.H. and Kim, E.O. (2011) Physiological response in abalone Haliotis discus hannai with different salinity. Korean Journal of Malacology, 27: 283-289.
Stohs, S.J., Bagchi, D., Hassoun, E. and Bagchi, M. (2000) Oxidative mechanism in the toxicity of chromium and cadmium ions. Journal of environmental Pathology Toxicology and Oncology, 19: 201-213.
Sugiyama, M. (1994) Role of cellular antioxidant in metal-induced damage. Cell Biology and Toxicology, 10: 1-22.
Wang, W., Mai, K., Zhang, W., Ai, Q., Yao, C., Li, H. and Liufu, Z. (2009) Effects of dietary copper on survival, growth and immune response of juvenile abalone, Haliotis discus hannai Ino. Aquaculture, 297: 122-127.
Wendel, A. and Feuerstein, S. (1981) Drug-induced lipid peroxidation in mice—I Modulation by monooxegenase activity, glutathione and selenium status. Biochemical Pharmacology, 30: 2513-2520.
Winston, G.W. and Di Giulio, R.T. (1991) Prooxidant and antioxidant mechanisms in aquatic organisms. Aquatic Toxicology, 19: 137-161.
Wo, K.T., Lam, P.K.S. and Wu, R.S.S. (1999) A comparison of growth biomarkers for assessing sublethal effects of cadmium on a marine gastropod, Nassarius festivus. Marine Pollution Bulletin, 39:165-173.