ISSN : 1225-3480
Gonad maturation of Manila clam, Ruditapes philippinarum was induced in this study using a recirculation system over 8 weeks in early spring. Clams used in the experiment wre collected in 15th April 2010 from the west coast of Korea, as the surface water temperature remained 11°C. To induce gametogenesis and subsequent maturation seawater temperature was elevated 1°C per day over 10 days to reach 20°C. For the experiment, clams were raised in 120 L quadrangle tank maintained with re-circulated seawater system over 57 days. Water quality parameters including the water temperature, salinity dissolved oxygen, ammonium ion and nitrate levels in the tanks were monitored daily. Mixture of concentrated microalgae including Tetraselmis, Isochrysis, Pavlova and Thalassiosira weissflogii was supplied to clams twice a day, and quantity of the daily ration was adjusted as 3% of clam body dry weight. Histology was applied to examine gonad maturation. Daily monitoring of the water quality parameters indicated that the recirculation system supplied suitable environment to Manila clam; the nitrogenous components stayed below toxic levels (< 0.2 mg/L). At the beginning of the study, clams were mostly in early developing stage. As the seawater temperature reached 20°C, 10 days after the experiment, 20% of clams reached late development at 12 days. First ripe clams were observed at 42 days and 40% of clams were in ripe and ready for spawning at the end of study, 57 days after the experiment. In this study, gametogenesis of Manila clam was successfully induced by elevating water temperature and supplying commercially produced microalgae in a recirculation tank system.
Allam B, Paillard C, Ford SE. (2002) Pathogenicity of Vibrio tapetis, the etiological agent of brown ring disease in clams. Diseases of Aquatic organisms, 48:221-231.
Buchanan, J.T., Roppolo, G.S., Supan, J.E., Tiersch, T.R. (1998) Conditioning of Eastern oyster in a closed, recirculating system. Journal of shellfish research, 17(4): 1183-1189.
Chung E.Y., Lee J.S., Lee C.H., Hur S.B. (2002)Reproductive cycle of natural population and artificial control of gonadal development of Ruditapes philippinarum by the conditions of water temperature-feeding and starvation. Korean journal of malacology, 18(2): 83-91. [in Korean with English abstract]
Drummond L, Mulcahy M, Culloty S. (2006) The reproductive biology of Manila clam, Ruditapes philippinarum, from the North-West of Ireland. Aquaculture, 254: 326-340.
Frias R and Segovia M. (2010) Gonad development of the Japanese oyster Crassostrea gigas in a recirculating system: First step toward the development of conditioning and maturation protocols. Journal of shellfish research, 29(2):303-308.
Helm M.M. and Bourne N. (2004) Hatchery operation:Broodstock conditioning, spawning, and fertilization. In: A. Lovatelli, editor. Hatchery culture of bivalve:A practical manual, FAO fisheries technical paper. pp. 1-471, Rome, Italy.
Hur Y.B., Min K.S., Kim T.E., Lee S.J., Hur S.B. (2008)Larvae growth and biochemical composition change of the Pacific oyster Crassostrea gigas, larvae during artificial seed production. Journal of the Korean Aquaculture Society, 21(4): 203-212. [in Korean with English abstract]
Isla Molleda, M. (2007) Water quality in recirculating aquaculture system for Arctic charr (Salvelinus alpinus L.) culture. pp. 1-54, The United Nations University, Iceland.
Keino H, Sugiyama I, Nishizawa T, Suzuki T. (2005)The study of relationship between burrowing behavior and energy consuming process of Japanese littleneck clam (Ruditapes philippinarum) at the stormy conditions in winter. Fisheries engineering, 42(1): 1-7. [in Japanese with English abstract]
KOSIS. (2003) Statistic Database for Reclamation Extent. Retrieved from http://kosis.kr/wnsearch/totalSearch.jsp
Lee Y.H., Chang Y.J., Lim H.K., Chung G.S. (1996)Comparison of growth and survival rate in Shortnecked clams, Ruditapes philippinarum from different seeding production areas. Journal of aquaculture, 9(3): 223-232. [in Korean with English abstract]
Marina D and Alejandro P.C. (2007) Influence of temperature on gonadal development of Ruditapes philippnarum (Adams and Reeve, 1850) with special reference to ingested food and energy balance. Aquaculture, 264: 398-407.
Mzighani S. (2005) Fecundity and population structure of cockles, Anadara antoquata L. 1758 (Bivalvia:Arcidae) from a sandy/muddy beach near Dares Salaam, Tanzania. Western indian ocean journal of marine science, 4(1): 77-84.
Ngo T.T.T., Berthe F.C., Berthe, Choi K.S. (2003)Prevalence and infection intensity of the ovarian parasite Marteilioides chungmuensis during an annual reproductive cycle of the oyster Crassostrea gigas. Diseases of Aquatic Organisms, 56(3): 259-267.
Ojea J, Pazos A.J., Martinez D, Novoa S, Garcia-martinez P, Sanchez J.L., Abad M. (2008)Effects of temperature regime on broodstock conditioning of Ruditapes decussates. Journal of shellfish research, 27(5): 1093-1100.
Prado S, Romalde J.L., Barja J. (2010) Review of probiotics for use in bivalve hatcheries. Veterinary microbiology, 145: 187-197.
Park K.I. and Choi K.S. (2001) Spatial distribution and infection intensity of the protozoan parasite Perkinsus sp. in the Manila clam Ruditapes philippinarum in Korea. Aquaculture, 203: 9-22.
Park K.I. and Choi K.S. (2004) Application of enzyme-linked immunosorbent assay for studying of reproduction in the Manila clam Ruditapes philinarum (Mollusca: Bivalvia) I. Quantifying eggs. Aquaculture, 241: 667-687.
Park K.I., Figueras A., Choi K.S. (2006) Application of enzyme-linked immunosorbent assay (ELISA) for the study of reproduction in the Manila clam Ruditapes philippinarum (Mollusca: Bivalve): Ⅱ. Impacts of Perkinsus olseni on clam reproduction. Aquaculture, 251: 182-191.
Park K.I., Yang H.S., Kang D.H., Choi K.S. (2010)Density dependent growth and mortality of Manila clam Ruditapes philippinarum reared in cages in Gomso-bay, Korea. Korean journal of malacology, 26(1): 91-95. [in Korean with English abstract]
Shi Y.K., Kim Y, Chung E.Y., Hur S.B. (2001) Effect of the dissolved oxygen concentration on the physiology of the Manila clam, Ruditapes philippinarum. Bulletin of the Korean fisheries society, 34(3):190-193.
Tun K.L., Shimizu Y.S., Yamanoi H, Yoshinaga T, Ogawa K. (2008) Seasonality in the infection and invasion of Marteilioides chungmuensis in the Pacific oyster Crassostrea gigas. Diseases of aquatic organisms, 80(2): 157-165.
Uddin, M.J., Yang, H.S., Choi, K.S., Kim, H.J., Hong, J.S., Cho, M. (2010) Seasonal changes in Perkinsus olseni infection and gametogenesis in Seonjaedo Island in Incheon, off the west coast of Korea. Journal of the world aquaculture society, 41(S1), 93-101.
Yan X, Zhang G, Yang F. (2006) Effects of diet, stocking density, and environmentalfactors on growth, survival, and metamorphosis of Manila clam Ruditapes philinarum larvae. Aquaculture, 253:350-358.
Yang H.S., Park K.J., Choi K.S. (2010) Pathologic survey on the Manila clam Ruditapes philinarum (Adams and Reeve 1850) from Haeju off the western coastal Yellow sea. Ocean science journal, 45(2): 93-100.
