ISSN : 1225-3480
본 연구는 구리에 노출된 꼬막, Tegillarca granosa의 생존율, 호흡율 및 기관계의 반응을 알아보고자 하였다. 실험기간은 4주였으며, 실험구는 대조구와 구리 노출구 3개 (0.125, 0.250 and 0.500 mg/L) 였다. 실험 결과 구리는 꼬막의 생존율과 호흡률의 저하 및 기관계의 조직학적 변성을 유도하는 것이 확인되었다. 노출 종말점에서 0.500 mg/L의 구리 노출구에서 사망률은 66.7%였으며, 호흡률은 대조구에 비해 18.2%로 감소하였다. 기관계의 조직학적 분석 결과, 외투막 상피층과 결합조직층의 변성, 아가미 새엽 상피세포의 위축과 측면섬모의 소실, 발에서는 상피층의 붕괴, 점액의 산성화 및 혈림프동의 확장과 근섬유 다발의 변성을 나타냈다. 소화맹낭의 소화선세관에서는 호염기성세포와 상피세포의 위축 및 붕괴가 확인되었다.
This study was conducted to find out the changes of survivorship, respiration and organ structure of Tegillarca granosa exposed to copper (Cu). Experimental period was four weeks. Experimental groups were composed of one control condition and three copper exposure conditions (0.125, 0.250 and 0.500 mg/L). The results of the study confirmed that copper induces reduction of survival rate and respiration rate and histopathology of organ structure of the bivalve. In the copper concentration of 0.500 mg/L, mortality was 66.7% after Cu exposure of 4 weeks. Respiration rate was observed exposure groups lower than control decline by 18%. Histological analysis of organ system illustrated degeneration of epithelial layer and connective tissue layer of the mantle. Also, histological degenerations as epithelial atrophy and disappearance of lateral cilia are recognized in the gill and it was observed expansion of hemolymph sinus, disruption of epithelial layer, acidification of mucous and degeneration of muscle fiber bundles in the foot. In the digestive diverticulum, it was showed atrophy and destruction of basophilic cell and epithelial cell in the digestive tubules.
Andhale, A.V., Bhosale, P.A., Zambare, S.P. (2011)Histopathological study of nickel induced alterations in the fresh water bivalve, Lammellidens marginalis. Journal of Experimental Sciences, 2: 1-3.
Beninger, P.G., Le Pennec, M., Salaun, M. (1988)New observations of the gills of Placopecten magellanicus (Mollusca: Bivalvia),, implications for nutrition. Marine Biology, 98: 61-70.
Cheung, S.G., Cheung, R.Y. (1995) Effects of heavy metals on oxygen consumption, ammonia excretion in green-lipped mussels (Perna viridis). Marine Pollution Bulletin, 31: 381-386.
Chin, P., Lee, J.A., Shin, Y.K., Lee, J.S. (1999)Environmental tolerance for pollutants in Littorina brevicula (Philippi). 2. The growth, metabolism, histological changes exposed to TBTCl, heavy metals in Littorina brevicula. Journal of Korean Fisheries Society, 32: 593-600. (in Korean)
Depledge, M.H., Hopkin, S.P. (1995) Methods to assess effects on brackish, estuarine, near-coastal water organisms. In: Methods to assess the effects of chemicals on ecosystems. (ed. by Linthurst, R.A., Bourdeau, P., Tardiff, R.G.). pp. 125-149. Wiley, Chichester, United Kingdom.
Eble, A.F. (2001) Anatomy, histology of Mercenaria mercenaria. In: Biology of the hard clam. (ed. by Kraeuter, J.N., Castagna, M.). pp. 117-220. Elsevier, New York.
Gregory, M.A., George, R.C., Marshall, D.J., Anandraj, A., Mcclurg, T.P. (1999) The effects of mercury exposure on the surface morphology of gill filament in Perna perna (Mollusca: Bivalvia). Marine Pollution Bulletin, 39: 116-121.
Hassan, B.A., Julia, E.B. (1980) Behavioral responses of the bivalve Scrobicularia plana (da Costa)subjected to short-term copper (Cu Ⅱ) concentrations. Marine Environmental Research, 4(2): 97-107.
Hebel, D.K., Jones, M.B., Depledge, M.H. (1997)Responses of crustaceans to contaminant exposure: a holistic approach. Estuarine, Coastal, Shelf Science, 44: 177-184.
Huggett, R.J., Kimerle, R.A., Mehrle, P.M. Jr, Bergman, H.L. (1992) Biomarkers: biochemical, physiological,, histological markers of anthropogenic stress. pp. 1-347, Lewis Publishers, Boca Raton, Florida.
Husmann, G., Abele, D., Monien, D., Monien, P., Kriews, M., Philipp, E.E.R. (2012) The influence of sedimentation on metal accumulation, cellular oxidative stress markers in the Antarctic bivalve Laternula elliptica. Estuarine, Coastal, Shelf Science, 111: 48-59.
James, G.W., Robert, F.M. (1981) Effects of high environmental copper concentration on the oxygen consumption, condition, shell morphology of natural populations of Mytilus edulis L., Littorina rudis. Comparative Biochemistry, Physiology, Part C: Comparative Pharmacology, 70(2):139-147.
Ju, S.M., Lee, J.W., Jin, Y.G., Yu, J., Lee, J.S. (2006) Effect of zinc bioaccumulation on survival rate, activity, growth, organ structure of the equilateral venus, Gomphina veneriformis (Bivalvia:Veneridae). Journal of Environmental Toxicology, 21:115-126.
Kang, J.C., Kim, H.Y., Chin, P. (1997) Toxicity of cooper, cadmium, chromium on survival, growth, oxygen consumption of the mysid, Neomysis awatschensis. Journal of Korean Fisheries Society, 30:874-881. (in Korean)
Kim, S.Y., Lee, T.Y. (1988) The effects of pollutants effluent from a steam-power plant on coastal bivalves. Ocean Research, 10: 47-56. (in Korean)
Krishnakumar, P.K., Asokan, P.K., Pillai, V.K. (1990)Physiological, cellular responses to copper, mercury in the green mussel Perna viridis (Linnaeus). Aquatic Toxicology, 18: 163-173.
Lee, J.S., Kang, J.C., Shin, Y.K., Ma, K.H., Chin, p. (2001) Histological responses of the flounder, Paralichthys olivaceus exposed to copper. Journal of Fish Pathology, 14(2): 81-90. (in Korean)
Lee, J.S., Park, J.J. (2007) Risk assessment of nonylphenol using the sex ratio, sexual maturation, intersex, lipofuscin accumulation of the equilateral venus, Gomphina veneriformis (Bivalvia:Veneridae). Journal of Korean Fisheries Society, 40:16-23. (in Korean)
Marin, M.G., Boscolo, R., Cella, A., Degetto, S., Ros, L.D. (2006) Field validation of autometallographical black silver deposit (BSD) extent in three bivalve species from the Lagoon of Venice, Italy (Mytilus galloprovincialis, Tapes philippinarum, Scapharca inaequivalvis) for metal bioavailability assessment. Science of Total Environment, 371: 156-167.
Moore, M.N., Allen, J.I. (2002) A computational model of the digestive gland epithelial cell of the marine mussel, its simulated responses to oil-derived aromatic hydrocarbons. Marine Environmental Research, 54: 579-584.
Morrison, C.M. (1993) Histology, cell ultrastructure of the mantle, mantle lobes of the eastern oyster, Crassostrea virginica, Gmelin: A summary atlas. American Malacological Bulletin, 10: 1-24.
NRC (National Research Council). (1983) Risk assessment in the federal government: managing the process. pp. 1-192, National Academy Press. Washington.
Otludil, B., Cengiz, E.I., Yildirim, M.Z., Unver, O., Unlu, E. (2004) The effects of endosulfan on the great ramshorn snail, Planorbariua corneus (Gastropoda, Pulmonata): a histopathological study. Chemosphere, 56: 707-716.
Park, K.Y., Lee, D.J., Lee, C.H., Won, D.H., Lee, W.H., Kwak, I.S. (2009) Studies on endopoints of toxicological evaluation of heavy metals in Brachinella kugenumaensis. Journal of Environmental Toxicology, 24(3): 241-249.
Park, S.J., Kim, B.J., Rhee, J.M. (2003) Antibacterial activity of activated carbon fibers containing copper metal. Polymer Korea, 27(3): 235-241.
Rojik, I., Nemcs'OK, J., Boross, L. (1983)Morphological, biochemical studies on liver, kidney, gill of fishes affected by pesticides. Acta Biological Hungarica, 34: 81-92.
Schintu, M., Durante, L., Maccioni, A., Meloni, P., Degetto, S., Contu, A. (2008) Measurement of environmental trace-metal levels in mediterranean coastal areas with transplanted mussels, DGT techniques. Marine Pollution Bulletin, 57: 832-837.
Shin, Y.K., Jun, J.C., Kim, E.O., Hur, Y.B. (2011)Physiological changes, energy budget of the sea squirt Halocynthia roretzi from Tongyeong, South Coast of Korea. Korean Journal of Fisheries, Aquatic Sciences, 44(4): 366-371.
Shin, Y.K., Park, J.J., Lim H.S., Lee, J.S. (2013)Changes of biomarker in manila clam, Ruditapes philippinarum exposed to lead. Korean Journal of Malacology, 29(1): 7-13.
Shin, Y.K., Park, J.J., Lim H.S., Lee, J.S. (2013)Copper toxicity on survival, respiration, organ structure of Mactra veneriformis (Bivalvia: Mactridae). Korean Journal of Malacology, 29(2): 129-137.
Soto, M., Cajaraville, M.P., Marigómez, I. (1996)Tissue, cell distribution of copper, zinc, cadmium in the mussel, Mytilus galloprovincialis, determined by autometallography. Tissue, Cell, 28: 557-568.
Sunila, I. (1988) Acute histological responses of the gill of the mussel, Mytilus edulis, to exposure by environmental pollutants. Journal of Invertebrate Pathology, 52: 137-141.
Sutherland, J., Major, C.W. (1981) Internal heavy metal changes as a consequence of exposure of Mytilus edulis, the blue mussle, to elenated external copper (Ⅱ) levels. Comparative Biochemistry, Physiology, 68: 63-67.
Vasanthi, L.A., Revathi, P., Arulvasu, C., Munuswamy, N. (2012) Biomarkers of metal toxicity, histology of Perna viridis from Ennore estuary, Chennai, south east coast of India. Ecotoxicology, Environmental Safety, 84: 92–98.
Viarengo, A. (1985) Biochemical effects of trace metals. Marine Pollution Bulletin, 16: 153-158.
Viarengo, A., Canesi, L., Pertica, M., Poli, G., Moore, M.N., Orunesu, M. (1990) Heavy metal effects on lipid peroxidation in the tissues of Mytilus galloprovincialis Lam. Comparative Biochemistry, Physiology, C, 97: 37-42.
Vijayavel, K., Gopalakrishnan, S., Balasubramanian, M.P. (2007) Sublethal effect of silver, chromium in the green mussel Perna viridis with reference to alterations in oxygen uptake, filtration rate, membrane bound ATPase system as biomarkers. Chemosphere, 69: 979–986.
Xiuling, J.I., Qunhui, S., Fang, L., Jing, M., Gang, X., Yuanlong, W., Minghong, W. (2012) Antibiotic resistance gene abundances associated with antibiotics, heavy metals in animal manures, agricultural soils adjacent to feedlots in Shanghai;China, Journal of Hazardous Materials, 235-236:178-185.
Zorita, I., Ortiz-Zarragoitia, M., Soto, M., Cajaraville, M.P. (2006) Biomarkers in mussels from a copper site gradient (Visnes, Norway): an integrated biochemical, histochemical, histological study. Aquatic Toxicology, 78: 109-116.
