ISSN : 1225-3480
The seven selected primers BION-13, BION-29, BION-61, BION-64, BION-68, BION-72 and BION-80 generated the total number of loci, average number of loci per lane and specific loci in Meretrix lusoria (ML), Saxidomus purpuratus (SP) and Cyclina sinensis (CS) species. Here, the complexity of the banding patterns varied dramatically between the primers from the three venerid clam species. The higher fragment sizes (> 1,000 bp) are much more observed in the SP species. The primer BION-68 generated 21 unique loci to each species, which were ascertaining each species, approximately 150 bp, 300 bp and 450 bp, in the ML species. Remarkably, the primer BION-80 detected 7 shared loci by the three clam species, major and/or minor fragments of sizes 500 bp, which were matching in all samples. As regards average bandsharing value (BS) results, individuals from CS clam species (0.754) exhibited higher bandsharing values than did individuals from SP clam species (0.607) (P < 0.05). In this study, the dendrogram obtained by the seven oligonucleotides primers indicates three genetic clusters: cluster 1 (LUSORIA01-LUSORIA07), cluster 2 (PURPURATUS08-PURPURATUS14), cluster 3 (SINENSIS15- SINENSIS21). Among the twenty one venerid clams, the shortest genetic distance that displayed significant molecular differences was between individuals 18 and 20 from the CS species (genetic distance = 0.071), while the longest genetic distance among the twenty-one individuals that displayed significant molecular differences was between individuals LUSORIA no. 02 and PURPURATUS no. 09 (genetic distance = 0.778). Relatively, individuals of SP venerid species were appropriately closely related to that of CS species, as shown in the hierarchical dendrogram of genetic distances. Eventually, PCR fragments exposed in the present study may be worthwhile as a DNA marker the three venerid clam species to discriminate.
Chenyambuga, S.W., Hanotte, O., Hirbo, J., Watts, P.C., Kemp, S.J., Kifaro, G.C., Gwakisa, P.S., Petersen, P.H., Rege, J.E.O. (2004) Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers. Asian-Australasian Journal of Animal Sciences, 17: 445-452.
Huang, B.X., Peakall, R., Hanna, P.J. (2000)Analysis of genetic structure of blacklip abalone (Haliotis rubra) populations using RAPD, minisatellite, microsatellite markers. Marine Biology, 136: 207-216.
Jeffreys, A.J., Morton D.B. (1987) DNA fingerprints of dogs, cats. Animal Genetics, 18: 1-15.
Jin, Y. G., Oh, B. S., Jung, C.K., Kim, T.I., Park, M.W. (2011) Survival, growth of the purplish Washington clam, Saxidomus purpuratus spat sowed in bottom, intermediate culture. Korean Journal of Malacology, 27: 199-204 (in Korean).
Ju, S. M., Lee, J. S. (2011) Ultrastructure of the Digestive Diverticulum of Saxidomus purpuratus (Bivalvia: Veneridae). Korean Journal of Malacology, 27: 159-165 (in Korean).
Jung, H.T., Kim, J., Shin, J.A., Sohn, H.Y., Choi, S.D. (2004) Genetic relationship of the five venerid clams (Bivalvia, Veneridae) in Korea. Journal of Aquaculture, 17: 251-257 (in Korean).
Kim, Y.H., Chang, D.S., Cha, B.R., Park, Y.C. (2001)Estimation of population size of Fulvia mutica (Reeve) in Gwangyang Bay. Abstracts 2001 Joint Meeting of the Korean Society on Fisheries Science:400-401 (in Korean).
Kim, S.J. Chung, Y.J., Lee, T.K. (2011) In vivo comet assay on flounder, clam exposed to BaP, TBT. Ocean, Polar Research, 33: 127-133 (in Korean).
Kim, Y.H., Kwon, D.H., Chang, D.S., Kim, J.B., Kim, S.T.and Ryu, D.K. (2007) Stock assessment of purplish Washington clam, Saxidomus purpuratus in the southern coastal waters of Korea. Korean Journal of Malacology, 23: 31-38 (in Korean).
Kim, J.Y, Park, C.Y., Yoon, J.M. (2004) Genetic differences, DNA polymorphism in oyster (Crassostrea spp.) analysed by RAPD-PCR. Korean Journal of Genetics, 26: 123-134.
Mahmoud, N., Dellali, M., El Bour, M., Aissa, P., Mahmoudi, E. (2010) The use of Fulvia fragilis (Mollusca: Cardiidae) in the biomonitoring of Bizerta lagoon: A multimarkers approach. Ecological Indicators, 10: 696-701.
McCormack, G. C., Powell, R, Keegan, B. (2000)Comparative analysis of two populations of the brittle star Amphiura filiformis (Echinodermata:Ophiuroidae) with different life history strategies using RAPD markers. Marine Biotechnology, 2:100-106.
Min, D.K. (2001) Korea mollusks with color illustration. Min shell house, pp. 267-275.
Park, S.Y., Park, J.S., Yoon, J.M. (2005) Genetic difference, variation in slipper lobster (Ibacus ciliatus), deep sea lobster (Puerulus sewelli)throughout its distribution range determined by RAPD-PCR Analysis. Korean Journal of Genetics, 27:307-317, 2005.
Shin, Y.K., Choi, N.J., Oh, B.S., Jung, A.J., Kim, S.Y. (2007) Gonad development, reproductive cycle of the purplish Washington clam, Saxidomus purpuratus Bivalvia: Veneridae) from Gangjin Bay. Korean Journal of Malacology, 23: 165-172 (in Korean).
Tassanakajon, A., Pongsomboon, S., Jarayabhand, P., Klinbunga, S., Boonsaeng, V. (1998) Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA?analysis. Journal of Marine Biotechnology, 6: 249-254.
Yoon, J.M., Kim, Y.H. (2003) Wide marsh clam (Corbicula spp.) populations from three sites analysed by RAPD-PCR-AGE. Bulletin of Electrochemistry, 19: 337-348.
Yoon, J.M., Kim, J.Y. (2004) Genetic differences within, between populations of Korean catfish (S. asotus), bullhead (P. fulvidraco) analyzed by RAPD-PCR. Asian-Australasian Journal of Animal Sciences, 17: 1053-1061.
Yoke-Kqueen, C., Radu, S. (2006) Random amplified polymorphic DNA analysis of genetically modified organisms. Journal of Biotechnology, 127: 161-166.
