ISSN : 1225-3480
본 연구는 북방전복의 생식생물학적 정보를 제공하고 아울러 성의 인위적인 조절에 필요한 정보를 제공하기 위하여 수행하였다. 북방전복의 형태학적 성분화 과정은 크게 다음과 같이 5단계로 구분할 수 있었다. 1) 생식소 외막 형성 (FGOM) (<TEX>${\leq}SL\;10.0{\pm}1.0mm$</TEX>), 2) 장과 간췌장 사이의 결체조직에 시원생식세포 (PGCs: primordial germ cells) 출현 (PAC) 및 생식소 내강 형성 (FGC) (SL <TEX>$15.0{\pm}2.0mm$</TEX>), 3) 생식소 내강 상피층에 PGCs 출현 (PAG) (SL <TEX>$18.0{\pm}2.0mm$</TEX>), 4) 생식세포형성소낭 형성, 초기 난모세포 및 정원세포 출현 (FGOC) (SL <TEX>$21.0{\pm}2.0mm$</TEX>), 5) 형태학적 성분화 (MSD) (<TEX>${\geq}SL\;23.0{\pm}2.0mm$</TEX>). 조직학적 분석 결과, 각장 24.1-25.0 mm 그룹에서 북방전복의 성분화율은 90.0%였으며, 성비 (암:수)는 1:0.8로 나타났다.
This study was conducted to provide the reproductive biological information and basic data on the artificial sex control of Haliotis discus hannai. The morphological sex differentiation process of H. discus hannai could be classified into following five phases: 1) formation of gonad outer membrane (FGOM) (≤ SL 10.0 ± 1.0 mm), 2) primordial germ cells (PGCs) appearance in the connective tissue between intestine and hepatopancreas (PAC), and formation of gonadal cavity (FGC) (SL 15.0 ± 2.0 mm), 3) PGCs appearance in the epithelial layer of gonadal cavity (PAG) (SL 18.0 ± 2.0 mm), 4) formation of gametogenic follicle and appearance of early oocytes and spermatogonia (FGOC) (SL 21.0 ± 2.0 mm), 5) morphological sex differentiation (MSD) (≥ SL 23.0 ± 2.0 mm). From histological analysis sex differentiation rate in SL 24.1-25.0 mm of H. discus hannai was 90.0% and sex ratio (female : male) was 1:0.8.
Awaji, M., Hamano, K. (2004) Gonad formation, sex differentiation, gonad maturation processes in artificially produced juveniles of the abalone, Haliotis discus hannai. Aquaculture, 239: 397–411.
Capinpin Jr., E.C., Encena Ⅱ, V.C., Bayona N.C. (1998) Studies on the reproductive biology of the Donkey’s ear abalone, Haliotis asinina Linné. Aquaculture, 166: 141-150.
Chávez-Villalba, J., Soyez, C., Huvet, A., Gueguen, Y., Lo, C., Le Moullac, G. (2011) Determination of gender in the pearl oyster Pinctada margaritifera. Journal of Shellfish Research, 30: 231-240.
Fretter, T. (1984) Prosobranchs. In: The Mollusca, Vol. 7. Reproduction. (ed. by Tompa, A.S., Verdonk, N.H., Van Den Biggelaar, J.A.M.). pp. 1-45. Academic Press, New York.
Jung, G.K., Park, J.J., Ju, S.M., Jin, Y.G., Lee, J.S. (2007) Ovarian structure, oogenesis of the spiny top shell, Batillus cornutus (Lightfoot, 1786)(Gastropoda: Turbinidae). The Korean Journal of Malacology, 23: 209-216.
Kim, J.W., Lee, B.W., Kang, J.C., Min, E.Y., Won, S.H., Lim, H.G., Kang, S.W., Jeon, M.A., Lee, J.S. (2015) Reproductive cycle of the abalone, Haliotis discus discus collected from Jeju Island of Korea. The Korean Journal of Malacology, 31: 21-26.
Lee, T.Y. (1974) Gametogenesis, reproductive cycle of abalones. Publications of the Marine Laboratory, Busan Fisheries College, 7: 21-50.
Najmudeen, T.M., Victor, A.C.C. (2004) Reproductive biology of the tropical abalone Haliotis varia from Gulf of Mannar. Journal of Marine Biological Association of India, 46: 154-161.
Park, M.W., Kim, H., Kim, B.H., Son, M.H., Choi, J.S. Choi, Lee, J.S. (2014) Reproductive cycle of the abalone, Haliotis discus hannai collected from Jindo of Korea. The Korean Journal of Malacology, 30:243-248.
Sobhon, P., Apisawetakan, S., Chanpoo, M., Wanichanon, C., Linthong, V., Thongkukiatkul, A., Jarayabhand, P., Kruatrachue, M., Upatham, S.E., Poomthong, T. (1999) Classification of germ cells, reproductive cycle, maturation of gonads in Haliotis asinina Linnaeus. ScienceAsia, 25: 3-21.
Visser-Roux, A. (2011) Reproduction of the South African abalone, Haliotis midae. Ph.D. Dissertation, pp. 88, University of Stellenbosch, Stellenbosch.
Webber, H.H., Giese, A.C. (1969) Reproductive cycle, gametogenesis in the black abalone, Haliotis cracheroidii (Gastropoda: Prosobranchiata). Marine Biology, 4: 152-159.
Wilson, N.H.F., Schiel, D.R. (1995) Reproduction in two speies of abalone (Haliotis iris, H. australis)in southern New Zealand. Marine, Freshwater Research, 46: 629-637.
Young, J.S. (1970) The reproductive cycle, gonadal histology, gametogenesis of the red abalone Haliotis rufescens (Swaison). California Fish, Game, 56: 298-309.
Yusa, Y. (2007) Causes of variation in sex ratio, modes of sex determination in the Mollusca - an overview. American Malacological Bulletin, 23: 89-98.