바로가기메뉴

본문 바로가기 주메뉴 바로가기

ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

logo

메뉴

이매패류 어미관리를 위한 미세조류 기반 폐쇄-순환여과시스템 개발 연구: Ⅰ. 성 성숙 유도

Development studies of microalgae-based closed recirculating bivalves adults conditioning system: Ⅰ. Induction of the gametogenesis

초록

폐쇄순환여과시스템을 활용 굴 어미를 전체 42일 간의 평균 일간 사육수의 교환율은 0.5%였고, 총 사육수 교환은 21.3%이었다. 실험기간 동안 수온은 실험개시 시 <TEX>$16.3^{\circ}C$</TEX>에서 서서히 증가시켜 실험 21일째 <TEX>$22.7^{\circ}C$</TEX>로 상승되었고, 이 후 실험종료 시까지 <TEX>$22.1{\pm}0.4^{\circ}C$</TEX>였고, 염분변화는 <TEX>$24.9{\pm}0.4$</TEX> 이었고, 용존산소는 7.9-5.1 mg/L 이었고, pH 는 <TEX>$7.93{\pm}0.15$</TEX>였다. 암모니아와 질산의 축적농도 범위는 각각 1.958-0.353 mg/L 과 1.34-0.47 mg/L 였고, 아질산 농도는 0.03-0.16 mg/L 였고, 용존성무기인의 농도는 0.42-0.03 mg/L, 규산염은 0.00-3.83 mg/L, 아질산을 제외하고 실험기간이 경과함에 따라 유의적(P < 0.05) 으로 감소하는 경향을 보였다. 생식소 발달은 실험 42일 만에 암컷은 90.9%, 수컷은 94.4% 방란 방정이 가능한 완전히 성숙된 생식소로 발달하였다. 비만도는 실험 개시 시 25.2에서 종료 시 24.5로 유의적인 차이는 없었고, 크기요소인 평균 각장과 각폭은 실험 개시 시에 약간 성장하였지만 유의적인 차이는 없었다 (P < 0.05). 그러나 평균 각고는 개시 시 대비 종료 시 8.3 mm 성장하였고, 무게요소인 평균 전중, 육중 및 각중은 전체적으로 유의적인 증가가 나타났다. 실험기간 동안 전체 평균 생존율은 98.7%였고, 각장은 실험개시 시 평균 54.5 mm 에서 실험종료 시 59.2 mm 로 유의적인 성장변화는 없었지만, 평균 4.6 mm 성장하였고, 각고는 실험 14일째 평균 122.9 mm 까지 유의적인 성장이 관찰되었다. 실험결과 충분히 본 시스템의 시설용량으로 기존 가온해수유수 사육 방법 대비 에너지비용과 먹이공급량 대비 10분 1수준에서 채란 가능한 어미로 성숙시킬 수 있을 것으로 추정되어 향 후 상업적 인공종묘배양장에서 충분히 활용 가능 할 것으로 판단된다.

keywords
Crassostrea gigas conditioning, gametogenesis, recirculation, microalgae-based

Abstract

Techniques were developed for holding and conditioning of Pacific oysters, Crassostrea gigas, in a closed recirculating system. Experimental adults were used 500 oysters ( x two system, total 1,000 oysters) which were collected in 20th March 2016 from long-line aquaculture farm at the south coast of Korea. During conditioning periods concentrated live microalgae as Isochrysis sp. 15 × 107 cells/mL, Tetraselmis sp. 2 × 107 cells/mL and Pheaodactylum sp. 18 × 107 cells/mL were added 5 L every day, respectively which micro algae were functioned as diets and biological filter. Over all experimental periods total water exchange rate was 21.3% (daily 0.5%). Over 42 days conditioning, female and male oysters were maturated 90.9% and 94.4%, respectively. Survival rate was 98.7%. Mean shell hight (8.3 mm), total wet weight (19.2 g), meat wet weight (5.0 g) and shell wet weight (13.6 g) were significantly increased (P〈 0.05). Water quality parameters including the water temperature (22.1 ± 0.4℃), salinity (24.9 ± 04), dissolved oxygen (5.1-7.9 mg/L) and pH (7.93 ± 0.15) were kept stable. Concentration of dissolved inorganic nutrient as ammonia (1.96-0.35 mg/L), nitrite (0.03-0.16 mg/L), nitrate (1.34-0.47 mg/L), DIP (0.42-0.03 mg/L) and silicate (3.83-0.00 mg/L) were significantly decreased throughout experiment except nitrite which was increased (P〈 0.05), but nitrogenous components stayed below toxic levels (ammonia 0.0-5.5 mg/L, nitrite 0.0-460.0 mg/L) which indicated that closed recirculation system with microalgae based bio-filter could supply sufficiently environment condition to holding and conditioning of oyster.

keywords
Crassostrea gigas conditioning, gametogenesis, recirculation, microalgae-based

참고문헌

1.

Bayne, B.L. and Scullard, C. (1977) Rates of nitrogen excretion by species of Mytilus (Bivalvia: Mollusca). Journal of the Marine Biological Association of the United Kingdom, 57: 355–369.

2.

Boulter, M. and Wilson, P. (1998) The use of physiological assessment techniques for determining the relative activity rates of bivalve shellfish during simulated depuration. Journal of Shellfish Research, 17: 1627–1631.

3.

Brune, D.E., Schwartz, G., Eversole, A.G., Collier, J.A., Schwedler, T.E. (2003) Intensification of pond aquaculture and high rate photosynthetic system. Aquacultural Engineering, 28: 65-86.

4.

Buchanan, J.T., Roppolo, G.S., Supan, J.E., Tiersch, T.R. (1998) Conditioning of Eastern oyster in a closed, recirculating system. Journal of shellfish research, 17(4): 1183-1189.

5.

Buzin, F. Dupuya B, Lefebvre S, Barillé L, Haure J. (2015) Storage of Pacific oysters Crassostrea gigas in recirculating tank: Ammonia excretion and potential nitrification rates, Aquacultureal Engineering, 64:8-14.

6.

Chung, E.Y., Hur, S.B., Hur, Y.B., Lee, J.S. (2001)Gonadal Maturation and Artificial Spawning of the Manila Clam, Ruditapes phijippinarum (Bivalvia:Veneridae), in Komso Bay. Korea Journal of Fisheries Science and Technology, 4(4): 208-218.

7.

Colt, J.E. and Armstrong, D.A. (1981) Nitrogen toxicity to crustaceans, fish, and molluscs. In: Allen, L.K.E.C. (Ed.), Bio-Engineering Symposium for Fish Culture. Bethesda,MD (USA) of the Fish Culture Section American Fisheries Society. Traverse City,MI (USA), pp. 34–47.

8.

De Abreu Corrêa, A., Dutra Albarnaz, J., Moresco, v., Rogerio Poli, C., Luiz Teixeira, A., Maria Oliveira Simoes, C., Regina Monte Barardi, C. (2007)Depuration dynamics of oysters (Crassostrea gigas)artificially contaminated by Salmonella enterica serovar Typhimurium. Marine Environmental Research, 63: 479-489.

9.

Emerson, D.N. (1969) Influence of salinity of ammonia excretion rates and tissue constituents of euryhaline invertebrates. Comparative Biochemistry and Physiology, 29: 1115–1133.

10.

Epifanio, C.E., Logan, C.M., Turk, C. (1975) Culture of six species of bivalves in a recirculating seawater system. Proceedings of the tenth European symposium on marine biology, Universa press, Wetteren. pp. 97-108.

11.

Epifanio, C.E. and Srna, R.F. (1975) Toxicity of ammonia, nitrite ion, nitrate ion and orthophosphate to Mercenaria mercnearia and Crasssotrea virginica. Marine Biology, 33: 241-246.

12.

Florence, B., Beatrice, D., Sebastien, L., Laurent, B., Joel H. (2015) Storage of Pacific oyster Crassostrea gigas in recirculating tank: ammonia excretion and potential nitrification rates. Aquacultural Engineering, 64: 8-14.

13.

Food and Agriculture Organization (FAO) of the United Nations, http://www.fao.org/ fishery /species/2669/en (Retrived online on January 28, 2011).

14.

Frias, R and Segovia, M. (2010) Gonad development of the Japanese oyster Crassostrea gigas in a recirculating system: First step toward the development of conditioning and maturation protocols. Journal of shellfish research, 29(2):303-308.

15.

Galtsoff, P. S. (1964). The American oyster, Crassostrea virginica Gmelin. Fishery Bulletin, 64: 1480.

16.

Hammen, C.S., Miller, H.F., Geer, W.H. (1966) Nitrogen excretion of Crassostrea virginica. Comparative Biochemistry and Physiology, 17: 1199–1200.

17.

Keppler, C.J. (2007) Effects of ammonia on cellular biomarker responses in oysters (Crassostrea virginica). Bulletin of Environmental Contamination and Toxicology, 78: 63–66.

18.

Flick Jr., G.J. (2013) Culture feasibility of eastern oysters (Crassostrea virginica) in zero-water exchange recirculating aquaculture systems using synthetically derived seawater and live feeds. Aquacultural Engineering, 54: 45–48.

19.

Lee, H.J., Park, K.L., Choi, K.S. (2014) Conditioning of Manila clam Ruditapes philippinarum (Adams & Reeve, 1850) using recirculation system: Ⅰ. Induction of the gametogenesis using water temperature elevation. The Korean Journal of Malacology, 30(2):127-134.

20.

Mac Millian, R.J., Cawthorn, R.J., Whyte, S.K., Lyon, P.R. (1994) Design and system maintenance of a closed artificial seawater system for long term holding of bivalve shellfish. Aquacultural Engineering, 13: 241–250.

21.

Martins, C.I.M., Eding, E.H., Verdegem, M.C.J., Heinsbroek, L.T.N., Schneider O., Blancheton, J.P., Roque d’Orbcastel, E., Verretham, J.A.J. (2010) New developments in recirculating aquaculture systems in Europe: A perspective on environmental sustainability. Aquacultural Engineering, 43(3): 83-93.

22.

Metaxa, E., Deviller, G., Pagand, P., Alliaume, C., Casellas, C., Blancheton, J.P. (2006) High rate algal pond treatment for water reuse in a marine fish recirculation system: Water purification and fish health. Aquaculture, 252: 92-101.

23.

Morales-Alamo, R. and Mann, R. (1989) Anatomical features in histological sections of Crassostrea virginica (Gmelin, l79l) as an aid in measurements of gonad area for reproductive assessment. Journal of shellfish research, 8: 71-82.

24.

Muñoz, R. and Guieysse, B. (2006) Algal-bacterial processes for the treatment of hazardous contaminants: A review. Water Resource, 40:2799-2815.

25.

Oswald, W. J. (1988) Large-scale algal culture systems (engineering aspects). In: Micro-algal biotechnology (Borowitzka M. A. and Borowitzka L. J.), Cambridge University Press, pp. 357-394.

26.

Roger, F. and Segovia, M. (2010) Gonad development of the Japanese oyster Crassostrea gigas in a recirculating system: first step toward the development of conditioning and maturation protocols. Journal of shellfish research, 29(2) :303-308.

27.

Shpigel, M., Barber, B.J., Mann, R. (1992) Effects of elevated temperature on growth, gametogenesis, physiology, and biochemical composition in diploid and triploid Pacific oysters, Crassostrea gigas Thunberg. Journal of Experimental Marine Biology and Ecology, 161: 15–25.

28.

Timmons, M.B. and Ebeling, J.M. (2007) Recirculating Aquaculture. Cayuga Aqua Ventures, Ithaca, NY.

29.

Wang, J. K. (2003) Conceptual design of a microalgae-based recirculating oyster and shrimp system. Aquacultural Engineering, 28: 37-46.

30.

Welsh, D.T. and Castadelli, G. (2004) Bacterial nitrification activity directly associated with isolated benthic marine animals. Marine Biology, 144: 1029–1037.

31.

경상남도. (2015) 해양수산현황, pp. 1-598.

32.

국토해양부. (2010) 해양환경공정시험기준, pp. 1-452.

logo