ISSN : 1225-3480
The selected seven oligonucleotides primers BION-32, BION-33, BION-35, BION-38, BION-40, BION-46 and BION-58 generated the shared loci, specific loci, unique shared loci to each population and shared loci by the three T. granosa populations in Beolgyo, a Chinese site and Wonsan, respectively. The bandsharing value between individuals’ no. 03 and no. 04 was 0.816, which was the highest value identified within the Beolgyo population. The primer BION-35 generated the most loci (a total of 70), with an average of 10.0 in the Wonsan population. On average, seven oligonucleotides primers generated 16.1 specific loci in the Beolgyo population, 22.3 in the Chinese population and 39.3 in the Wonsan population. 126 unique shared loci to each population, with an average of 18 per primer, were observed in the Beolgyo population, 63 loci, with an average of 9 per primer, were observed in the Chinese population, and 49 loci, with an average of 7 per primer, and were observed in the Wonsan population. The oligonucleotides primer BION-32 generated 14 unique loci to each population, which were identifying each population in the Beolgyo population. Interestingly, every primer had not distinguished the shared loci by the three populations, major and/or minor fragments of sizes, which were identical in almost all of the samples. As regards average bandsharing value (BS) results, individuals from Beolgyo population (0.717 ± 0.057) exhibited higher BS values than did those from Wonsan population (0.552 ± 0.104) (P < 0.05). The dendrogram resulted from truthful seven oligonucleotides primers, representing three genetic clusters comprising group I (BEOLGYO 01, 02, 03, 04, 05, 06 and 07), group II (CHINESE 08, 09, 10, 11, 12, 13 and 14) and group III (WONSAN 15, 16, 17, 18, 19, 20 and 21). In three T. granosa populations, the longest genetic distance (0.874) displaying significant molecular difference was also between individual no. 02 within the Beolgyo population and individual no. 12 within the Chinese population. Relatively, individuals of the CHINESE population were fairly closely related to those of the WONSAN population.
Chang, D.S., Moon, T.S. and Jung, M.M. (2006) Shell height frequency using of age and growth of blood cockle, Tegillarca granosa (Linnaeus) in Yeoja Bay, southern coast of Korea. Journal of Korean Aquaculture Society, 22: 135-141.
Chenyambuga, S.W., Hanotte, O., Hirbo, J., Watts, P.C., Kemp, S.J., Kifaro, G.C., Gwakisa, P.S., Petersen, P.H. and Rege, J.E.O. (2004) Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers. Asian-Australasian Journal of Animal Sciences, 17: 445-452.
Huang, B.X., Peakall, R. and Hanna, P.J. (2000) Analysis of genetic structure of blacklip abalone (Haliotis rubra) populations using RAPD, minisatellite and microsatellite markers. Marine Biology, 136: 207-216.
Jeffreys, A.J. and Morton D.B. (1987) DNA fingerprints of dogs and cats. Animal Genetics, 18: 1-15.
Kang, K.H., Park, H.U. and Kim, J.M. (2000) Growth and optimal environmental factors of cockle shell, Anadara granosa bisenensis, spat in laboratory culture. Korean Journal of Malacology, 16: 25-29.
Kang, K.H., Kim, J.M. and Kim, Y.H. (2004) Influence of water temperature and salinity on oxygen consumption and filtration rate of ark shell, Anadara granosa bisenensis. Korean Journal of Malacology, 20: 107-110.
Kim, J.Y, Park, C.Y. and Yoon, J.M. (2004) Genetic differences and DNA polymorphism in oyster (Crassostrea spp.) analysed by RAPD-PCR. Korean Journal of Genetics, 26: 123-134.
Kim, S.Y., Moon, T.S. and Park, M.S. (2009) Gonadal development and reproductive cycle of the granular ark, Tegillarca granosa (Bivalvia: Arcidae). Journal of Korean Aquaculture Society, 22: 34-41.
Ku, K.Y., Ju, S.M. and Lee, J.S. (2015) Microanatomy of the heart-kidney complex of Tegillarca granosa (Bivalvia: Arcidae). Korean Journal of Malacology, 31: 291-298.
Lee, J.H. (1997) Studies on the gonadal development and gametogenesis of the granulated ark, Tegillarca granosa (Linne). Korean Journal of Malacology, 13:55-64.
McCormack, G. C., Powell, R and Keegan, B. (2000)Comparative analysis of two populations of the brittle star Amphiura filiformis (Echinodermata:Ophiuroidae) with different life history strategies using RAPD markers. Marine Biotechnology, 2:100-106.
Moon, T.S. and Shin, Y.K. (2010) Effect of salinity on survival and metabolism of ark shell, Tegillarca granosa. Korean Journal of Malacology, 26: 171-177.
Muchmore, M.E., Moy, G.W., Swanson, W.J. and Vacquier, V.D. (1998) Direct sequencing of genomic DNA for characterization of a satellite DNA in five species of Eastern Pacific abalone. Molecular Marine Biology and Biotechnology, 7: 1-6.
Park, S.Y., Park, J.S. and Yoon, J.M. (2005) Genetic difference and variation in slipper lobster (Ibacus ciliatus) and deep sea lobster (Puerulus sewelli)throughout its distribution range determined by RAPD-PCR Analysis. Korean Journal of Genetics, 27: 307-317, 2005.
Shin, Y.K., Park, J.J., Ju, S.M. and Lee, J.S. (2015)Copper toxicity on survival, respiration and organ structure of Tegillarca granosa (Bivalvia: Arcidae). Korean Journal of Malacology, 31: 151-158.
Song, Y.J. and Yoon, J.M. (2013) Genetic differences of three Pollicipes mitella populations identified by PCR analysis. Development & Reproduction, 17: 199-205.
Tassanakajon, A., Pongsomboon, S., Jarayabhand, P., Klinbunga, S. and Boonsaeng, V. (1998) Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA analysis. Journal of Marine Biotechnology, 6: 249-254.
Yoke-Kqueen, C. and Radu, S. (2006) Random amplified polymorphic DNA analysis of genetically modified organisms. Journal of Biotechnology, 127: 161-166.
Yoon, J.M. and Kim, G.W. (2003) Genetic differences between cultured and wild penaeid shrimp (Penaeus chinensis) populations analysed by RAPD-PCR. Korean Journal of Genetics, 25: 21-32.
Yoon, J.M. and Kim, J.Y. (2004) Genetic differences within and between populations of Korean catfish (S. asotus) and bullhead (P. fulvidraco) analyzed by RAPD-PCR. Asian-Australasian Journal of Animal Sciences, 17: 1053-1061.
