open access
메뉴ISSN : 1225-3480
This is the first report on the introduced foreign species Mercenaria mercenaria in Korea after being changed to a newly given Korean name (scientific name). Imports of this cultured species from China are increased rapidly for food in Korean market. In this study, for the purpose of the managing genetic resources we used CO1 and 16s rRNA universal primer for genetic classification of this species and conducted the phylogenetic analysis on the nineteen species similarly related species with M. mercenaria. The similarities with M. stimpsoni which belongs to the same genus as M. mercenaria were 90.8% (16s) and 81.7% (COI). The similarity with Meretrix petechialis was 56.6% (16s) and 75% (COI), which was relatively very low. Therefore, it is assumed that the probability of genetic disturbances with similar species is low. In the near future, M. mercernaria is expected to be used as biological resources for the mudflats on the west coast in Korea.
Barucca, M., Olmo, E., Schiaparelli, S., & Canapa, A. (2004) Molecular phylogeny of the family Pectinidae (Mollusca: Bivalvia) based on mitochondrial 16S and 12S rRNA genes. Molecular phylogenetics and evolution, 31(1): 89-95.
Canapa, A., Barucca, M., Marinelli, A., & Olmo, E. (2000) Molecular data from the 16S rRNA gene for the phylogeny of Pectinidae (Mollusca: Bivalvia). Journal of Molecular Evolution, 50(1): 93-97.
Canapa, A., Schiaparelli, S., Marota, I., & Barucca, M. (2003) Molecular data from the 16S rRNA gene for the phylogeny of Veneridae (Mollusca: Bivalvia). Marine Biology, 142(6): 1125-1130.
Castagna, M. (2001) Chapter 15 Aquaculture of the hard clam, Mercenaria mercenaria. In Developments in Aquaculture and Fisheries Science (Vol. 31, pp. 675-699). Elsevier.
Cross, I., Portela-Bens, S., García-Angulo, A., Merlo, M. A., Rodríguez, M. E., Liehr, T., & Rebordinos, L. (2018) A preliminary integrated genetic map distinguishes every chromosome pair and locates essential genes related to abiotic adaptation of Crassostrea angulata/gigas. BMC genetics, 19(1): 104.
Erpenbeck, D., Hooper, J. N. A., & Wörheide, G. (2006)CO1 phylogenies in diploblasts and the ‘Barcoding of Life’-are we sequencing a suboptimal partition?. Molecular ecology notes, 6(2): 550-553.
FAO (2004) Cultured Aquatic Species Information Programme. Mercenaria mercenaria. Cultured Aquatic Species Information Programme. Text by Kraeuter, J. N. In FAO Fisheries and Aquaculture Department [online]. pp. 11. Rome.
FAO (2016) The State of World Fisheries and Aquaculture 2016. Contributing to food security andmutrition for all. In FAO (Series Ed.), pp. 200.
Feng, Y., Li, Q., Kong, L., & Zheng, X. (2011) DNA barcoding and phylogenetic analysis of Pectinidae (Mollusca: Bivalvia) based on mitochondrial COI and 16S rRNA genes. Molecular Biology Reports, 38(1):291-299.
Hebert, P. D., Cywinska, A., Ball, S. L., & Dewaard, J. R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences, 270(1512): 313-321.
Hebert, P. D. N., Stoeckle, M. Y., Zemlak, T. S., &Francis, C. M. (2004) Identification of birds through DNA barcodes. Public Library of Science Biology, 2:e312.
Ivanova, N. V., Dewaard, J. R., & Hebert, P. D. (2006). An inexpensive, automation-friendly protocol for recovering high-quality DNA. Molecular ecology notes, 6(4): 998-1002.
Kocher, T. D., Thomas, W. K., Meyer, A., Edwards, S. V., Pääbo, S., Villablanca, F. X., & Wilson, A. C. (1989) Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proceedings of the National Academy of Sciences, 86(16): 6196-6200.
Kumar, S., Stecher, G., & Tamura, K. (2016) MEGA7:molecular evolutionary genetics analysis version 7.0for bigger datasets. Molecular biology and evolution, 33(7): 1870-1874.
Lee, J. S., & Min, D. K. (2007) Korean Names of Foreign Shells Introduced to Korea. The Korean Journal of Malacology, 23(1): 105-154.
Linnaeus, C. V. (1758) Systema Naturae, edition X, vol. 1 (Systema naturae per regna tria naturae, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis. Tomus I. Editio decima, reformata). Holmiae Salvii, 824.
Lunt, D. H., & Hyman, B. C. (1997) Animal mitochondrial DNA recombination. Nature, 387(6630):247-247.
National Fishery Products Quality Management Service, "import and export quarantine statistics", accessed NOV 21, 2020, https://www.nfqs.go.kr/2013/contents.asp?m=5&s=8&s2=3
Needleman, S. B., & Wunsch, C. D. (1970) A general method applicable to the search for similarities in the amino acid sequence of two proteins. Journal of molecular biology, 48(3): 443-453.
Ogunlaja, A., Sharma, V., Ghai, M., & Lin, J. (2020)Molecular characterization and DNA methylation profile of Libyodrilus violaceous from oil polluted soil. Molecular biology research communications, 9(2):45-53.
Palumbi, S. R. (1996) Nucleic acids II: the polymerase chain reaction. Molecular systematics, 205-247.
Park, S. Y., Kang, S. W., Hwang, H. J., Chung, J. M., Song, D. K., Park, H. S., ... & Lee, Y. S. (2016) A mitochondiral cytochrome oxidase I gene based identification of Corbicula ssp. commercially available in South Korea. The Korean Journal of Malacology, 32(2): 127-131.
Park, Y. J., Noh, E. S., Park, J. Y., Kim, E. M., Noh, J. K., Choi, T. J., & Kang, J. H. (2018) Development of a Simple, Rapid Multiplex PCR Method Using the 16S rRNA Gene to Determine the Country of Origin of Brackish Water Bivalve Corbicula japonica. Food Analytical Methods, 11(11): 3034-3041.
Pujolar, J. M., Marčeta, T., Saavedra, C., Bressan, M., &Zane, L. (2010) Inferring the demographic history of the Adriatic Flexopecten complex. Molecular Phylogenetics and Evolution, 57(2): 942-947.
Qiu, F., Guo, L., Wen, T. J., Liu, F., Ashlock, D. A., &Schnable, P. S. (2003) DNA Sequence-Based “Bar Codes” for Tracking the Origins of Expressed Sequence Tags from a Maize cDNA Library Constructed Using Multiple mRNA Sources. Plant Physiology, 133(2): 475-481.
Salvi, D., Bellavia, G., Cervelli, M., & Mariottini, P. (2010) The analysis of rRNA sequence-structure in phylogenetics: An application to the family Pectinidae (Mollusca: Bivalvia). Molecular Phylogenetics and Evolution, 56(3): 1059-1067.
Sastry, A. N. (1979) Pelecypoda (excluding Ostreidae), in; Reproduction of marine invertebrates. Vol. V. Molluscs: pelecypods and lesser classes.
Saraste, M. (1990) Structural features of cytochrome oxidase. Quarterly reviews of biophysics, 23(4):331-366.
Trontelj, P., Machino, Y., & Sket, B. (2005) Phylogenetic and phylogeographic relationships in the crayfish genus Austropotamobius inferred from mitochondrial COI gene sequences. Molecular Phylogenetics and Evolution, 34(1): 212-226.
Vences, M., Thomas, M., Van der Meijden, A., Chiari, Y., & Vieites, D. R. (2005) Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. Frontiers in zoology, 2(1): 1-12.
Yang, H., Sturmer, L. N., & Baker, S. (2016) Molluscan shellfish aquaculture and production (FA191), Gainesville: University of Florida Institute of Food and Agricultural Sciences. ,1-8.
Tao, Z. H. A. N. G., Sheng, Y. H., Zhong, L. B., Quan, L. B., Lin, L. S., Xiang, W. H., ... & Sui, Z. F. (2003) EFFECTS OF ENVIRONMENTAL FACTORS ON THE SURVIVAL AND GROWTH OF JUVENILE HARD CLAM Mercenaria mercenaria (LINNAEUS, 1758). Oceanologia Et Limnologia Sinica, 2: 2.
Zhi-Hua, L., Zhen-Ming, L., Xue-Liang, C., Jun, F., &Jiong-Ming, Z. (2008) Karyotypes of diploid and triploid Mercenaria mercenaria (Linnaeus). Journal of Shellfish Research, 27(2): 297-300.