ISSN : 1225-3480
In this study, we conducted a larval breeding study of Tresus keenae using 5 Bacillus genera, which are excellent in the ability to decompose high molecular weight organic matter and the antibacterial activity of pathogenic bacteria, among the 65 Bacillus genera. Research results that the relationship between larval growth and viability was high in the TKI42 and microbial mixed strains of TKI02 + TKI14 + TKI26 + TKI32 + TKI42. The growth and viability of larvae at the amount of the mixed microbial strain (TKI02 + TKI14 + TKI26 + TKI32 + TKI42) was 1.0×106 CFU/ml, which was the best growth, and 1.0×107 CFU/ml, which was the lowest. And the survival rate was the best at 1.0×105 CFU/ml. In addition, the growth and survival rate were high when the mixed microbial strain (1.0×106 CFU/ml) with a water temperature of 25°C was treated. In this study, when the growth and survival of larval breeding was investigated based on five Bacillus strains, the prediction of the application to the breeding of organisms in the aquaculture industry based on the special ability of a single strain is uncertain. Rather than developing and using only a single strain, it is thought that the effect can be expected to using mixed strains.
Abriouel, H., C.M. Franz, N.B. Omar. and A. Gálvez. (2010) Diversity and applications of Bacillus bacteriocins. FEMS. Microbiol. Rev., 35: 201-232.
Bidhan, C.D., D.K. Meena, B.K. Behera, P. Das, P.D. Mohapatra. and A.P. Sharma. (2014) Probiotics in fish and shellfish culture: immunomodulatory and ecophysiological responses. Fish. Physiol. Biochem., 40: 921-971.
Cahill, M.M. (1990) Bacterial flora of fishes: a review. Microb. Ecol., 19: 21-41.
Cleveland, J., T.J. Montville, I.F. Nes. and M.L. Chikindas. (2001) Bacteriocins: safe, natural antimicrobials for food preservation. Int. J. Food. Microbiol., 71: 1-20.
Chapple, J.P., G.R. Smerdon, R.J. Berry. and A.J.S. Hawkins. (1998) Seasonal changes in stress-70protein levels reflect thermal tolerance in the marine bivalve Mytilus edulis L. J. Exp. Mar. Biol. Ecol., 229: 53-68.
Ivanova, E.P., M.V. Vysotskii, V.I. Svetashev, O.I. Nedashkovskaya, N.M. Gorshkova, V.V. Mikhailov, N. Yumoto, Y. Shiger, T. Taguchi. and S. Yoshikawa. (1999) Characterization of Bacillus strains of marine origin. Int. Microbiol., 2: 267-271.
Kang, H.S. and C.W. Kim (2018) Effects of Water Temperature, Salinity, Rearing Density and Food Supply on the Growth and Survival of the Surf Clam, Tresus keenae Larvae. JMLS., 3: 67-73.
Ki, J.S., W. Zhang. and P.Y. Qian. (2009) Discovery of marine Bacillus species by 16S rRNA and rpoB comparisons and their usefulness for species identification. J. Microbiol. Methods., 77: 48-57.
Kim, J.H., Y.H. Kim, S.K. Kim, B.W. Kim. and S.W. Nam. (2011) Properties and industrial applications of seaweed polysaccharidesdegrading enzymes from the marine microorganisms. Microbiology and Biotechnology Letters, 39: 189-199.
Lee, H. and H.Y. Kim. (2011) Lantibiotics, class I bacteriocins from the genus Bacillus. J. Microbiol. Biotechnol., 21: 229-235.
Lee, J.G., G.J. Lee. and S.M. Lim. (2005) Partial purification of bacteriocin produced by Enterococcus faecium MJ-14 isolated from Meju. J. Food. Hyg. Saf., 20: 211-216.
Loomis, S.H., A.D. Ansell,. R.N. Gibson. and M. Barnes. (1995) Freezing tolerance of marine invertebrates. Oceanogr. Mar. Biol. Annu. Rev., 33: 337-350.
Min, B.H. (2019) Growth and Survival on Rearing Conditions and Live Food for Larvae of the Keen's gaper Tresus keenae. Korean J. Malacol., 35: 9-17.
Muñoz-Atienza, E., B. Gómez-Sala, C. Araújo, C. Campanero, R. Del Campo, P.E. Hernández, C. Herranz. and L.M. Cintas. (2013) Antimicrobial activity, antibiotic susceptibility and virulence factors of lactic acid bacteria of aquatic origin intended for use as probiotics in aquaculture. BMC. Microbiol., 13: 15.
Newell, R.C. and L.H. Kofoed. (1977) Adjustment of the components of energy balance in the gastropod Crepidula fornicate in response to thermal acclimation. Mar. Boil., 44: 275-286.
Park, J.Y., W.S. Kim, H.Y. Kim and E.H. Kim. (2016)Potential use of Bacillus amyloliquefaciens as a probiotic bacterium in abalone culture. J. Fish Pathol., 29: 35-43.
Shin, S., K. Yundendorj, S.S. Lee, K.H. Kang. and H.Y. Kahng. (2013) Physiological and Biochemical Characterization of Bacillus spp. from Polychaete, Perinereis aibuhitensis. J. Life Sci., 23: 415-425.
Shin, Y.K. and C.H. Wi. (2004) Effect of temperature and salinity on survival and metabolism of the hard shelled mussel Mytilus coruscus, Bivalve: Mytilidae. J. of Aquaculture, 17: 103-108.
Xie, F., T. Zhu, F. Zhang, K. Zhou, Y. Zhao. and Z. Li. (2013) Using Bacillus amyloliquefaciens for remediation of aquaculture water. Springerplus, 2:119.
Yang, E.J., J.Y. Chang, H.J. Lee, J.H. Kim, D.K. Chung, J.H. Lee. and H.C. Chang. (2002) Characterization of the antagonistic activity against LactoBacillus plantarum and induction of bacteriocin production. Korean J. Food Sci. Technol., 34: 311-318.
Ziaei-Nejad, S., M.H. Rezaei, G.A. Takami, D.L. Lovett, A.R. Mirvaghefi and M. Shakouri. (2006) The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus. Aquaculture, 252: 516-524.
