ISSN : 1225-3480
About 85,000 species of gastropods are known around the world, and the imports rate about edible gastropods are on the rise every year in Korea. The system will be needed to reflect taxonomic diversity about similar gastropoda groups. Because inaccurate information about edible species can cause serious problems in market. In this study, to cope with this problem, a study was conducted to accurately identify gastropods, and selected six target country with a large imported rate of edible gastropods in Korea. The collected gastropods were classified into 8 orders, 22 families and 33 species, including unrecorded in Korea, and were identified by morphological classification. Sequence analysis of the COI gene was performed for all collected species by COI universal primer. The result of molecular phylogenetic analysis and morphological classification result are similar. As a result of this study, it was confirmed that most gastropoda species can be identified using universal primers for gastropod COI sequences, and will be used as data for species identification of gastropods in the future.
Borges, L.M.S., Hollatz, C., Lobo, J., Cunha, A.M., Vilela, A.P., Calado, G., Coelho, R., Costa, A.C., Ferreira, M.S.G., Costa, M.H., and Costa, F.O. (2016)With a little help from DNA barcoding: investigating the diversity of Gastropoda from the Portuguese coast. Scientific Reports, 6: 20226.
Chee, S.Y., and Mohd Nor, S.A. (2016) DNA barcoding reveals neritid diversity (Mollusca: Gastropoda)diversity in Malaysian waters. Mitochondrial DNA A DNA Mapp Seq Anal., 27: 2282-2284.
Ewing, B., and Green, P. (1998) Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res., 8: 186-194.
Ewing, B., Hillier, L., Wendl, M.C., and Green, P. (1998)Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res., 8:175-185.
Galan, G., Mendez, N., and Dela Cruz, R. (2018) DNA Barcoding of three Selected Gastropod Species using Cytochrome Oxidase (COI). Gene, 21: 93-102.
Habe, T., and Ito, K. (1965) Shell of the world in color, 1 (reprinted in 1979) pp. 176. Hoikusha Pub. Co. Osaka,.
Higgins, D.G., and Sharp, P.M. (1988) CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene, 73: 237-244.
Huang, X., and Madan, A. (1999) CAP3: A DNA sequence assembly program. Genome Res., 9: 868-877.
Kang, S.W., Park, S.Y., Hwang, H.J., Chung, J.M., Sang, M.K., Min, H.R., Park, J.E., Cho, H.C., Patnaik, B.B., and Lee, Y.S. (2019) PANM DB ver 3.0 : An update of the bioinformatics database for annotation of large datasets from sequencing of species under Protostomia clade. The Korean Journal of Malacology, 35: 73-75.
Kumar, S., Stecher, G., Li, M., Knyaz, C., and Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol. Biol. Evol., 35: 1547-1549.
Kwon, O.K., Park, G.M., and Lee, J.S. (1993) Coloured Shells of Korea pp. Academy Publishing Company.
Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J., and Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics, 23:2947-2948.
Lee, J.S., and Min, D.K. (2002) A Catalogue of Molluscan Fauna in Korea. The Korean Journal of Malacology, 18:
Lee, Y.-S., Jo, Y.-H., Kim, D.-S., Kim, D.-W., Kim, M.-Y., Choi, S.-H., Yon, J.-O., Byun, I.-S., Kang, B.-R., Jeong, K.-H., and Park, H.-S. (2004) Construction of BLAST Server for Mollusks. The Korean Journal of Malacology, 20: 165-169.
Lobo, J., Costa, P.M., Teixeira, M.A.L., Ferreira, M.S.G., Costa, M.H., and Costa, F.O. (2013) Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans. BMC Ecology, 13:34.
Ma, X. (1997) Fauna Sinica pp. Chineses Academy of Sciences, Beijing.
Min, D.K., Lee, J.S., Koh, D.B., and Je, J.K. (2004)Mollusks in Korea. pp. 566. Min Molluscan Research Institute.
Okada, Y., Uchida, S., and Uchida, T. (1967) New illustrated encyclopedia of the fauna of Japan (II)pp. Hokuryukan Co., LTD, Tokyo.
Okutani, T.E. (2000) Marine Mollusks in Japan. pp. 1172. Tokai University.
Qi, Z., Ma, X., Zhang, F., and Lou, Z. (1983) 中國動物圖鑑. 軟体動物 第 2冊 pp. 150. 科學出版社, 北京.
Qi, Z.e. (2004) Sea shells of China. pp. China Oceon Press, Beijing,.
Qi, Z.Y., Ma, X.T., Wang, Z.R., Ling, G.Y., Xu, F.S., Dong, Z.Z., Li, F.L., and Lü, R.H. (1989) Mollusca of Huanghai and Bohai. pp. 309-323. Agricultural Publishing House, BeiJing.
Remigio, E.A., and Hebert, P.D. (2003) Testing the utility of partial COI sequences for phylogenetic estimates of gastropod relationships. Mol. Phylogenet Evol., 29: 641-647.
Tamura, K., and Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol., 10: 512-526.
Wu, W.L., and Lee, Y.C. (2005) The Taiwan Common Mollusks In Color. pp. Academica Sinica, Taiwan.
이준상 (2015) 국가 생물종 목록 - 무척추동물-Ⅵ [연체동물-Ⅱ(복족류, 미공류, 다판류, 이매패류, 굴족류, 두족류)] pp. 환경부 국립생물자원관.
