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Determination of Glutathione in Biological Samples by Ion-pairing HPLC/FLD
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1999, v.12 no.1, pp.28-33
Yoo,
J., Lee,
K., &
Shin,
H.
(1999). Determination of Glutathione in Biological Samples by Ion-pairing HPLC/FLD. Analytical Science and Technology, 12(1), 28-33.
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Abstract
Glutathione(GSH) in biological samples was determined by high performance liquid chromatographic(HPLC) method with fluorescence detector(FLD) after monobromobimane(MBB) or 4-fluoro-7-sulfobenzofurazan(SBD-F) derivatization. The detection limit of <TEX>$0.03{\mu}g/mL$</TEX> was obtained after MBB derivatization, derivative of MBB was about 200 times more sensitive than that of SBD-F. N-acetylcysteine was used as internal standard and tetrabutylammonium ion as counter ion for better separation. The determination by ion-pairing chromatography after MBB derivatization was characterized by linearity in the range between <TEX>$0.08{\sim}8.33{\mu}g/mL$</TEX> with a good correlation coefficient of 0.998. By precision test appeared relative standard deviation at less than 5% at three different concentrations. This method can be used for the analysis of GSH in plasma and tissue.
- keywords
- glutathione, HPLC/FLD, monobromobimane, plasma, rat liver tissue
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