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- E-ISSN 2288-8985
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Gender Determination of X and Y-Specific Alphoid Repeat Sequences by PCR
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1999, v.12 no.1, pp.80-83
Choi,
D., Kang,
P., Lee,
Y., &
Han,
M.
(1999). Gender Determination of X and Y-Specific Alphoid Repeat Sequences by PCR. Analytical Science and Technology, 12(1), 80-83.
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Abstract
Recently, it has been possible to the alphoid gene, which has X and Y specificity, and determine the sex from human physical evidence using PCR methods. Samples from single sources, PCR method applied to the alphoid gene results in highly sensitive and accurate results even when only 60 pg of the genomic DNA was available for sex determination. Even for samples containing DNA from more than one gender source where the female DNA was present in the amount 10 times than that of the male, sex determination was possible. Therefore, this result suggests that alphoid gene, which has X and Y specificity, could be used effectively for sex determination in case of mixed DNA samples from biological evidence.
- keywords
- gender analysis, alphoid gene, PCR, human DNA
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