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Profiling of Urinary Environmental Estrogens by Gas Chromatography/Mass Spectrometry
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1999, v.12 no.4, pp.265-272
Yang,
Y., Lee,
S., &
Chung,
B.
(1999). Profiling of Urinary Environmental Estrogens by Gas Chromatography/Mass Spectrometry. Analytical Science and Technology, 12(4), 265-272.
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Abstract
A simultaneous profile analysis of 19 environmental estrogens, which act like estrogen and may effect the endocrine system by binding to hormone receptors or influencing cell signaling pathways, was attempted. The present method was based on the selected ion monitoring (SIM) mode of gas chromatography/mass spectrometry (GC/MS). It involves solid-liquid extraction, enzyme hydrolysis, liquid-liquid extraction and quantitative conversion into trimethylsilyl (TMS)-ether derivatives. Analytical recovery range was 47.6 ~ 99.5% and the RSD values of within-a-day and day-to-day test were 0.66 ~ 9.33%, 1.66 ~ 16.14%, respectively. The Korean reference values for the evaluation of environmental estrogen effects were established by this method.
- keywords
- environmental estrogen, gas chromatography/mass spectrometry
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