- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Log In/Sign Up
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Changes of Silk Protein Compositions by Solubility Condition
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1999, v.12 no.4, pp.306-311
Yeo,
J., Lee,
K., Lee,
Y., Nam,
J., &
Kim,
S.
(1999). Changes of Silk Protein Compositions by Solubility Condition. Analytical Science and Technology, 12(4), 306-311.
- Downloaded
- Viewed
Abstract
Changes of silk protein compositions of average molecular weight (Mw) and free amino acid composition to different solubility conditions were studied by SDS-polyacrylamide electropholesis, gel permeation chromatography (GPC), and free amino acid analysis method. We can not detected average molecular weight distribution of different hydrochloric acid (HCl) conditions as SDS-polyacrylamide method, but as using GPC method, molecular weight distribution of 2N-HCl, 1N-HCl and 0.5N-HCl (3 hrs at <TEX>$110^{\circ}C$</TEX> treated) are confirmed Mw 800, 1,500 and 3,700, respectively. The average molecular weight of calcium chroride and calcium chloride-enzyme treated samples are shown Mw 46,800 and 12,500, respectively. The degree of hydrolysis and the composition of the free amino acid in the fibroin hydrolysates effected significantly composition of free amino acids of the fibroin powder. The increase of the degree of hydrolysis and ratio of free amino acids and oligopeptides were found to be directly related to the concentration of hydrochloric acid and treatment of enzyme, resulting in the increase of water solubility.
- keywords
- silk protein, molecular weight distribution, free amino acid
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Profiling of Urinary Environmental Estrogens by Gas Chromatography/Mass Spectrometry
- Simultaneous Determination of Mercury and Arsenic by Reductive Vapor Generation-ICP-AES
- The Determination of <TEX>$^{14}C$</TEX> in Stack Effluent Gases by Carbonate Suspension Counting Method
- A Study on the Quantitative Recovery of Dissolved Inorganic Carbonates in Ground Water for Radiocarbon Measurement
- A Study on the Precise End-Point Detection in Titration by Using the Phase Angle Measurements
- Fundamental Studies on the Development of On-line Monitoring of Trace Mercury in Drinking Water
- Changes of Silk Protein Compositions by Solubility Condition
- Quality Control on the BOD, COD, TN, TP of Water Quality Pollutant
- Analysis of Sludges from Wastewater of Hanji Production
- Analysis of the Metabolites of 1,2,4-Trimethylbenzene by Capillary Electrophoresis
- Construction and Evaluation of Cryogenic Preconcentration System for Analysis of VOC with ppb Level in the Air
- Simultaneous Analysis of Inorganic Cations by Capillary Zone Electrophoresis
- Data intercomparison and determination of toxic and trace elements in Algae using Instrumental Neutron Activation Analysis
- Review : Characteristics of Gas Chromatograph - Atomic Emission Detector ( GC-AED )
- Characteristics of Gas Chromatograph-Atomic Emission Detector(GC-AED)
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.