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Alignment change of lipid molecules in lipid bilayers by an antimicrobial peptide protegrin-1
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2015, v.28 no.2, pp.106-111
https://doi.org/10.5806/AST.2015.28.2.106
(2015). Alignment change of lipid molecules in lipid bilayers by an antimicrobial peptide protegrin-1. Analytical Science and Technology, 28(2), 106-111, https://doi.org/10.5806/AST.2015.28.2.106
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Abstract
Changes in antimicrobial peptide-lipid mixtures were investigated using 31P solid-state nuclear magnetic resonance spectroscopy. An antimicrobial peptide, protegrin-1, and phosphatidylcholine were deposited on a thin cover glass and incubated under a relative humidity of 95%. The changes in the mixtures were observed after hydration or air-drying. How repetitive hydration and drying changed the phase of the sample was also observed. The degrees of disruption of the well-aligned bilayers of phosphatidylcholine were determined quantitatively by simulating the experimental spectra. The peptide-lipid mixtures changed reversibly after hydration and drying, and the samples reached an equilibrium state after several repetitions.
- keywords
- antimicrobial peptide, protegrin-1, hydration, phase change
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