- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
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Establishment and application of a qualitative real-time polymerase chain reaction method for detecting genetically modified papaya line 55-1 in papaya products
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2015, v.28 no.2, pp.117-124
https://doi.org/10.5806/AST.2015.28.2.117
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(2015). Establishment and application of a qualitative real-time polymerase chain reaction method for detecting genetically modified papaya line 55-1 in papaya products. Analytical Science and Technology, 28(2), 117-124, https://doi.org/10.5806/AST.2015.28.2.117
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Abstract
Genetically modified (GM) papaya line 55-1, which is resistant to PRSV infection, has been marketed globally. Prompt and sensitive protocols for specific detections are essential for the traceability of this line. Here, an event- and construct-specific real-time polymerase chain reaction (RT-PCR) method was established to detect 55-1. Qualitative detection was possible for fresh papaya fruit up to dilutions of 0.005% and 0.01% for the homozygous SunUp and heterozygous Rainbow cultivars, respectively, in non-GM papaya. The method was applied in the qualitative detection of 55-1 in eight types of commercially processed papaya products. Additionally, papaya products were monitored to distinguish GM papaya using the P35S and T-nos RT-PCR detection methods. As expected, detection capacity was improved via modified sample preparation and the established RT-PCR detection method. Taking these results together, it can be suggested that a suitable method for the extraction and purification of DNA from processed papaya products was established for the detection of GM papaya.
- keywords
- genetically modified, real-time polymerase chain reaction, papaya, 55-1, monitoring
Reference
1
1. J. Clive, ISAAA Briefs, 44, 2012.
2
2. G. Jinchao, Y. Litao, L. Xin, Z. Haibo, Q. Bingjun and Z. Dabing, J. Agric. Food Chem., 57, 6502-6509 (2009).
3
3. D. Gonsalves, S. Tripathi, J. Carr and J. Suzuki, The American Phytopathological Society, Available from: http://www.apsnet.org/edcenter/intropp/lessons/viruses/Pages/PapayaRingspotvirus.aspx, Accessed 10 Jan 2013.
4
4. G. Fermin, P. Tennant, C. Gonsalves and D. Lee, Methods Mol. Biol., 286, 399-430 (2005).
5
5. N. Gryson, Anal. Bioanal. Chem., 396, 2003-2022 (2010).
6
6. G. Fermin, R. Keith, J. Suzuki, S. Ferreira, D. Gaskill and K. Pitz, J. Agric. Food Chem., 59, 10006-10012 (2011).
7
7. N. Kosuke, A. Hiroshi, T. Yuki, K. Tomoko, N. Akio, O. Kiyomi, K. Masaki, K. Kazumi, N. Hiroyuki, K. Kazunari and T. Reiko, Food Chem., 136, 895-901 (2013).
8
8. K. Nakamura, H. Akiyama, K. Ohmori, Y. Takahashi, R. Takabatake and K. Kitta, Biol. Pharm. Bull, 34, 1648-1651 (2011).
9
9. H. Waiblinger and B. Ernst, A., Eur. Food Res. Technol., 226, 1221-1228 (2008).
10
10. R. Reiting, H. Broll, H. Waiblinger and L. Grohmann, J. Verbrauch. Lebensm., 27, S2-75-S2-81 (2007).
11
11. O. Kiomi, N. Kosuke, K. Masaki, T. Reona, K. Kazumi, F. Teruhisa, K. Kazunari, T. Reiko and A. Hiroshi, Food Control, 10, 1016 (2013).
12
12. E. M. Tecson, A. C. Laurena and J. R. Botella, Biotechnol. Annual Review, 14, 423-462 (2008).
13
13. O. Jaroslava and H. Jan, Czech J. Food Sci., 27, S2-75- S2-81 (2009).
14
14. F. Min-Jen F, C. Shu, K. Yi-Jung, C. Ying-Huey, B. Huey-Jiunn, S. Tien-Tsai and Y. Shyi-Dong, Transgenic Res., 18, 971-986 (2009).
15
15. M. Evelyn Mae Tecson M, C. L. Antonio and B. Jose Ramon, Biotechnol. Annual Review, 14, 1387-2656 (2008).
16
16. M. Fitch, R. Manshardt, D. Gonsalves, J. Slightom and J. Sanford, Nat. Biotechnol., 10, 1466-1472 (1992).
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