- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Determination of terbutaline in human plasma by coupled column chromatography
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2015, v.28 no.2, pp.125-131
https://doi.org/10.5806/AST.2015.28.2.125
&
(2015). Determination of terbutaline in human plasma by coupled column chromatography. Analytical Science and Technology, 28(2), 125-131, https://doi.org/10.5806/AST.2015.28.2.125
- Downloaded
- Viewed
Abstract
A method was developed and fully validated for the determination of terbutaline, a β2-receptor agonist, in human plasma. Plasma samples were prepared by solid-phase extraction with Sep-Pak silica, followed by high-performance liquid chromatography (HPLC). The terbutaline was pre-separated from the interfering components in plasma on a Luna C18 (2) column, and terbutaline and salbutamol as an internal standard were resolved and determined on a Luna Silica column. The two columns were connected by a switching valve equipped with silica pre-column. The pre-column was used to concentrate the terbutaline in the eluent from the C18 column before back-flushing onto the silica column with fluorescence detection at an excitation/emission wavelength of 276/306 nm. The method was shown to be specific by testing six different human plasma sources. Linearity was established for a concentration range of 0.4-20.0 ng/mL with a correlation coefficient of 0.9999. The lower limit of quantitation was 0.4 ng/mL with a precision of 10.1% as C.V.%.
- keywords
- terbutaline, coupled column chromatography, HPLC, solid phase extraction, plasma
Reference
1
1. M. T. Acekrmans, J. L. Beckers, F. M. Everaerts and I. G. J. A. Seelen, J. Chromatogr., 590, 341-353 (1992).
2
2. G. A. Jacobson and G. M. Peterson, J. Pharm. Biomed. Anal., 12, 825-832 (1994).
3
3. United States Pharmacopeia (USP30) and National formulary (NF 25), United State Pharmacopeial Convention, Rockville, MD (2007).
4
4. British Pharmacopoeia (BP 2013), British Pharmacopoeia Commission, Market Towers, London (2013).
5
5. R. A. Clare, D. S. Davies and T. A. Baillie, Biomed. Mass. Spectrom., 6, 31-37 (1979).
6
6. S. E. Jacobsson, S. Jonsson, C. Lindberg and L. A. Svensson, Biomed. Mass. Spectrom., 7, 265-268 (1980).
7
7. M. Caban, P. Stepnowski, M. Kwiatkowski, N. Migowska and J. Kumirska, J. Chromatogr. A., 1281, 8110-8122 (2011).
8
8. V. L. Herring and J. A. Johnson, J. Chromatogr. B., 741, 307-312 (2000).
9
9. Y. Q. Xia, D. Q. Liu and R. Bakhtiar, Chirality, 14, 742-749 (2002).
10
10. T. Zhou, T. Zhao, Q. Cheng, S. Liu, L. Xu and W. Tan, Biomed. Chromatogr., 28, 994-1002 (2014).
11
11. P. T. McCarthy, S. Atwal, A. P. Sykes and J. G. Ayres, Biomed. Chromatogr., 7, 25-28 (1993).
12
12. K. H. Kim, H. J. Kim, S. P. Hong and S. D. Shin, Arch. Pharm. Res., 23(5), 441-445, (2000).
13
13. Y. Arai, T. Fukushima, M. Shirao, X. Yang and K. Imai, Biomed. Chromatogr., 14, 118-124 (2000).
14
14. R. Wyss, F. Bucheli and W. Philipp, J. Chromatogr. A., 870, 189-198 (2000).
15
15. K. H. Kim, H. J. Kim, J. H. Kim and S. D. Shin, J, Chromatpgr. B., 751, 69-77 (2001).
16
16. Y. Zhang and Z. R. Zhang, J. Chromatpgr. B., 805, 211-214 (2004).
Other articles from this issue
- The preliminary evaluation of semi-quantitative analysis by in situ pre-chromatographic derivatization of amines and image analysis in TLC
- Development of a LC–MS/MS method for simultaneous analysis of 20 antihistamines in dietary supplements
- Dopamine determination using a biosensor based on multiwall carbon nanotubes paste and burley tobacco-peroxidase
- Alignment change of lipid molecules in lipid bilayers by an antimicrobial peptide protegrin-1
- Simultaneous determination of betaine and choline using derivatization by HPLC with UV detection
- Establishment and application of a qualitative real-time polymerase chain reaction method for detecting genetically modified papaya line 55-1 in papaya products
- Determination of terbutaline in human plasma by coupled column chromatography
- Comparison of rosiglitazone metabolite profiles in rat plasma between intraperitoneal and oral administration and identifcation of a novel metabolite by liquid chromatography-triple time of flight mass spectrometry
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.