- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
The preliminary evaluation of semi-quantitative analysis by in situ pre-chromatographic derivatization of amines and image analysis in TLC
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2015, v.28 no.2, pp.79-85
https://doi.org/10.5806/AST.2015.28.2.79
&
(2015). The preliminary evaluation of semi-quantitative analysis by in situ pre-chromatographic derivatization of amines and image analysis in TLC. Analytical Science and Technology, 28(2), 79-85, https://doi.org/10.5806/AST.2015.28.2.79
- Downloaded
- Viewed
Abstract
A preliminary experiment was performed to develop a fast, convenient, and economical semi-quantitative method of analyzing amphetamine-like amines from images of derivatives. These were generated from the reaction (in situ, co-spot) of three amphetamine-like compounds with three derivatization reagents on a TLC plate. The attempt was made to optimize the reaction conditions for an efficient derivatization reaction, and TLC images taken by a digital camera were analyzed using two types of image analysis program (CP Atlas 2.0 and ImageJ) for repeatability (RSD, %) and linearity (R2). Then, their results were compared. For efficient derivatization, the reaction conditions needed to be modified. The results of image analysis of each of the samples at two different concentrations (0.5 mg/mL and 0.01 mg/mL) showed that the RSD values for reaction repeatability were in the range of 0.69-5.50%. From the calibration curves between the area of the derivative and the concentration of amines, the R2 values (R2 > 0.9906) for good linear correlation were found to be high, in a concentration range of 0.1-0.005 mg/mL of amines. In addition, the two programs demonstrated little difference in the analysis of repeatability and linearity of the derivatization, so that the current method has the potential to be used for the semi-quantitative analysis of amines.
- keywords
- TLC, in situ derivatization, repeatability, linearity, image analysis
Reference
1
1. J. M. P otka, M. Biziuk and C. Morrison, TrAC Trends in Anal. Chem., 30(7), 1139-1158 (2011).
2
2. N. Kato, Science & Justice, 41(4), 239-244 (2001).
3
3. E. Deconinck, P. Y. Sacr, P. Courselle and J. O. De Beer, J. Chromatogr. Sci., 51, 791-806 (2013).
4
4. G. Gbitz and R. Wintersteiger, J. Anal. Toxicol., 4(3), 141-144 (1980).
5
5. R. A. de Zeeuw, J. Hartstra and J. P. Franke, J. Chromatogr. A, 674(1), 3-13 (1994).
6
6. R. Kasar, A. Gogia, K. Shah, V. Anand and C. Anand, RRJPA, 2(4), 1-8 (2013).
7
7. C. Tistaert, B. Dejaegher and Y. Vander Heyden, Anal. Chim. Acta., 690, 148-161 (2011).
8
8. S. A. Kustrin and C. G. Hettiarachchi, Modern Chem. & Application, 2(1), e118 (2014).
9
9. D. Casoni and C. Srbu, Talanta, 114, 117-123 (2013).
10
10. E. Reich and A Schibli, In ‘High-performance thinlayer chromatography for the analysis of medicinal plants’, Thieme Medical Publishers, New York, USA (2006).
11
11. B. L. Ling, W. R. G. Baeyens, B. Del Castillo, K. Stragier, H. Marysael and P. De Moerloose, J. Pharm. Biomed. Anal., 7, 1671-1678 (1989).
12
12. R. M. Linares, J. H. Ayala, A. M. Afonso and V. Gonzalez, Analyst., 123, 725-729 (1998).
13
13. L. A. Barret, A. Polidori, F. Bonnete, P. Bernard-Savary and C. Jungas, J. Chromatogr. A, 1281, 135-141 (2013).
14
14. P. C. Lindholm, J. S. Knuutinen, H. S. Ahkola and S. H. Herve, BioResources, 9(2), 3688-3732 (2014).
15
15. P. Leroy, A. Nicolas and A. Moreau, J. Chromatogr., 282, 561-569 (1983).
16
16. C. R. Clark and M. M. Wells, J. Chromatogr. Sci., 16, 332-339 (1978).
17
17. S. Wawrzycki, E. Pyra and B. Wawrzycki, J. Planar Chromatogr., 14, 21-23 (2001).
18
18. S. Hernndez-Cassou and J. Saurina, J. Chromatogr. B, 879(17), 1270-1281 (2011).
19
19. B. L. Ling, W. R. G. Baeyens, B. Del Castillo, K. Stragier, H. Marysael and P. De Moerloose, J. Pharm. Biomed. Anal., 7, 1671-1678 (1989).
20
20. G. Maeder, M. Pelletier and W. Haerdi, J. Chromatogr., 593, 9-14 (1992).
21
21. S. W. Choi, H. I. Lee and N. D. Sung, Anal. Sci. & Tech., 26(4), 228-234 (2013).
22
22. M. B. Gawande and P. S. Branco, Green Chem., 13(12), 3355-3359 (2011).
23
23. H. Yamada, A. Yamahara, S. Yasuda, M. Abe, K. Oguri, S. Fukushima and S. Ikeda-Wada, J. Anal. Toxicol., 26(1), 17-22 (2002).
24
24. Y. S. Kim and S. W. Choi, Kor. J. Sci. Crim. Investig., 8(1), 31-36 (2014).
25
25. R. Kubec and E. Dadkov, J. Chromatogr. A, 1216(41), 6957-6963, (2009).
26
26. E. De Mey, G. Drabik-Markiewicz, H. De Maere, M. C. Peeters, G. Derdelinck, H. Paelinck and T. Kowalska, Food Chem., 130, 1017-1023 (2012).
27
27. S. W. Choi, S. H. Oh and N. D. Sung, Korean J. Sci. Crimi. Investig., 7(2), 89-96 (2013).
28
28. A. V. Irish Hess, J. Chem. Edu., 84(5), 842-847 (2007).
29
29. D. S. Maruti and S. K. Banerjee, J. Res. Pharm. Sci., 4(2), 310-315 (2013).
30
30. J. Saminathan and T. Vetrichelvan, KMITL Sci. Tech. J., 11(2), 54-63 (2011).
Other articles from this issue
- The preliminary evaluation of semi-quantitative analysis by in situ pre-chromatographic derivatization of amines and image analysis in TLC
- Development of a LC–MS/MS method for simultaneous analysis of 20 antihistamines in dietary supplements
- Dopamine determination using a biosensor based on multiwall carbon nanotubes paste and burley tobacco-peroxidase
- Alignment change of lipid molecules in lipid bilayers by an antimicrobial peptide protegrin-1
- Simultaneous determination of betaine and choline using derivatization by HPLC with UV detection
- Establishment and application of a qualitative real-time polymerase chain reaction method for detecting genetically modified papaya line 55-1 in papaya products
- Determination of terbutaline in human plasma by coupled column chromatography
- Comparison of rosiglitazone metabolite profiles in rat plasma between intraperitoneal and oral administration and identifcation of a novel metabolite by liquid chromatography-triple time of flight mass spectrometry
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.