- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
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HPLC-UV검출방법으로 유도체화를 통한 비테인과 콜린의 동시분석
Simultaneous determination of betaine and choline using derivatization by HPLC with UV detection
분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2015, v.28 no.2, pp.112-116
https://doi.org/10.5806/AST.2015.28.2.112
(서울여자대학교)
(연세대학교)
(연세대학교)
이인숙,
&
팽기정.
(2015). HPLC-UV검출방법으로 유도체화를 통한 비테인과 콜린의 동시분석. , 28(2), 112-116, https://doi.org/10.5806/AST.2015.28.2.112
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Abstract
Extraction of quaternary ammonium compounds (choline and betaine) from plant samples (spinach) using ion exchange resin (AG1, OH− form) is a very simple and inexpensive approach. However, it is very hard to determine amounts of choline and betaine simultaneously using high-performance liquid chromatographyultraviolet (HPLC-UV) detection. Unlike choline, betaine has low molar absorptivity in UV-visible (UV-Vis) region, which makes it difficult to carry out UV-Vis detection of betaine. The mixture of quaternary ammonium compounds (choline and betaine) was derivatized using 2-bromo acetophenone as a derivatizing agent. As a result, choline did not react with the derivatizing agent, whereas betaine formed a betaine derivative. This betaine derivative exhibited detectable UV absorption with baseline separation between choline and the betaine derivative. Thus, with this method, choline and betaine can be determined simultaneously by using the HPLCUV method through one-step derivatization, which is an easy, sensitive, and reliable method.
- keywords
- choline, betaine, derivatization, HPLC, UV detection
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