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- E-ISSN 2288-8985
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Analysis of selenoaminoacids and selenoproteins in blood serum of sows fed by selenium fortified feed
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2015, v.28 no.3, pp.196-203
https://doi.org/10.5806/AST.2015.28.3.196
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(2015). Analysis of selenoaminoacids and selenoproteins in blood serum of sows fed by selenium fortified feed. Analytical Science and Technology, 28(3), 196-203, https://doi.org/10.5806/AST.2015.28.3.196
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Abstract
Selenium species (inorganic selenium, selenoaminoacids, and selenoproteins) were analyzed using anion exchange and affinity chromatography, which were connected to ICP/MS for the blood serum of sows fed by seleniumfortified feed. The Anion Exchange PRP X-100 column was used for the analysis of inorganic selenium (Se4+ and Se6+) and selenoaminoacids. The HEP column was used to separate SelP from GPx+SeAlb in selenoproteins. A quantitative analysis was performed using the post-column isotope dilution technique. The lactating sows were divided into three groups and fed by selenium fortified feed (organic 0.3 mg/kg, 0.6 mg/kg and inorganic 0.6 mg/kg) for four weeks. The test groups showed increases in selenoaminoacids compared with the control group, except the inorganic feed group. There was no significant difference between the organic feed groups. All test groups showed increases in selenoproteins. In particular, SelP showed a large increase that was 1.5 times higher than the other proteins.
- keywords
- sow blood serum, AE & AF HPLC-ICP/MS, selenoproteins, selenoaminoacids, selenium fortified feed
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