- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Log In/Sign Up
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Characteristics of <TEX>$G_{418}$</TEX>-sensitive mitochondrial ATPase/ATP synthase from pleurotus florida
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1992, v.5 no.4, pp.477-484
Kim,
J., Kim,
D., Lee,
J., Lee,
S., &
Min,
T.
(1992). Characteristics of <TEX>$G_{418}$</TEX>-sensitive mitochondrial ATPase/ATP synthase from pleurotus florida. Analytical Science and Technology, 5(4), 477-484.
- Downloaded
- Viewed
Abstract
The mitochondrion was purified at 44% sucrose layer from pleurotus florida by using ultracentrifuge and sucrose density gradient method. Optimum pH and temperature of ATPase and ATP synthase were pH 7.4, <TEX>$60^{\circ}C$</TEX> and pH 7.5, <TEX>$57^{\circ}C$</TEX> respectively, also their Km values were determined as 11.6mM and 8.4mM. ATPase was activated at 5~6mM ATP substrate concentration, then ATP synthase was 5~10mM range ADP. ATPase/ATP synthase were <TEX>$Mg^{2+}$</TEX>-dependent enzyme, partially inhibited by their substrate, and then showed an none competitive inhibition pattern by <TEX>$G_{418}$</TEX>. Amino acid composition of ATPase/ATP synthase was as follows, hydrophobic amino acid residue was 50.5%, small residue, 56.1%, hydrogen bonding residue, 43.7% and helix breaking residue, 55.2%. Phosphatidyl choline, phosphatidyl ethanolamine and phosphatidyl glycerol were contained but not phosphatidyl inositol and phosphatidyl serine. Palmitate(51.31%), stearate(18.32%) and unsaturated fatty acids(<TEX>$C_{18:1}$</TEX>, <TEX>$C_{18:2}$</TEX> and <TEX>$C_{16:1}$</TEX>) were predominated.
- keywords
- ATPase, ATP synthase, pleurotus florida
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- The role of matrix modifier for the determination of Lead (Pb) in blood by graphite furnace atomic absorption spectrometry
- Micro Raman Spectroscopic Analysis of Local Stress on Silicon Surface in Semiconductor Fabrication Process
- Response Analysis of the NE213-PSD System for Neutron Energy Spectreum Measurement
- The Study on the Thermal Isomerization of Pinane
- Study on the Radiocarbon Dating with Liquid Scintillation Counting
- Elution Behavior of Pd(II) - Isonitrosoethylacetoacetate Imine Chelates by Reversed Phase High Performance liquid Chromatography
- Ion-Selectrodes based on Calcium Ferrite
- A Study on the Precursor of <TEX>$BaTiO_3$</TEX> Synthesized in Water
- Analysis of Arsenic(III) by the Cathodic Stripping Voltammetry
- Flotation-Concentration of Trace Phosphate Ion in Water Samples by <TEX>$La(OH)_3$</TEX> Coprecipitation
- A Study of Chemical Properties and Fusibility of Korean Anthracite Coal Ash
- A Study on the Hydrolysis and Analysis of o-Chlorobezylidenemalononitrile
- Fourier Transform Infrared Spectroscopic Analysis of the Silylated Resist on Silicon Wafers in Semiconductor Lithographic Process
- Assay of <TEX>$\beta$</TEX>-Galactosidase Using High Performance Liquid Chromatography
- Quantitative Analysis of Microencapsulation of <TEX>$\beta$</TEX>-Galactosidase
- Characteristics of <TEX>$G_{418}$</TEX>-sensitive mitochondrial ATPase/ATP synthase from pleurotus florida
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.