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open access
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ISSN : 1225-3480
In the present study, the seven primers BION-33, BION-34, BION-37, BION-41, BION-44, BION-45 and BION-42 generated the total number of loci, average number of loci per lane and specific loci in Hongseong, Yeosu and Goheung population of F. mutica, respectively. 7 primers generated 19 specific loci in the Hongseong population, 29.3 in the Yeosu population and 23.1 in the Goheung population, respectively. Especially, the decamer primer BION-37 generated 7 unique loci to each population, which were identifying each population, approximately 700 bp in Hongseong population. In this study, the dendrogram obtained by the seven primers indicates three genetic clusters: cluster 1 (HONGSEONG 01-HONGSEONG 07), cluster 2 (YEOSU 08-YEOSU 14) and cluster 3 (GOHEUNG 15-GOHEUNG 21). Among the twenty one cockles, the shortest genetic distance that displayed significant molecular differences was between individuals 17 and 19 from the Goheung population (genetic distance = 0.051), while the longest genetic distance among the twenty-one cockle individuals that displayed significant molecular differences was between individuals HONGSEONG no. 03 and YEOSU no. 12 (genetic distance = 0.616). Relatively, individuals of YEOSU population were fairly closely related to that of GOHEUNG population. Ultimately, PCR fragments revealed of in this study may be useful as a DNA marker the three geographic populations to distinguish.
Chenyambuga, S.W., Hanotte, O., Hirbo, J., Watts, P.C., Kemp, S.J., Kifaro, G.C., Gwakisa, P.S., Petersen, P.H. and Rege, J.E.O. (2004) Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers. Asian-Australasian Journal of Animal Sciences, 17: 445-452.
Huang, B.X., Peakall, R. and Hanna, P.J. (2000) Analysis of genetic structure of blacklip abalone(Haliotis rubra) populations using RAPD, minisatellite and microsatellite markers. Marine Biology, 136: 207-216.
Jeffreys, A.J. and Morton D.B. (1987) DNA fingerprints of dogs and cats. Animal Genetics, 18: 1-15.
Kim, Y.H., Chang, D.S., Cha, B.R. and Park, Y.C. (2001) Estimation of population size of Fulvia mutica (Reeve) in Gwangyang Bay. Abstracts 2001 Joint Meeting of the Korean Society on Fisheries Science: 400-401 (in Korean).
Kim, J.Y, Park, C.Y. and Yoon, J.M. (2004) Genetic differences and DNA polymorphism in oyster (Crassostrea spp.) analysed by RAPD-PCR. Korean Journal of Genetics, 26: 123-134.
Mahmoud, N., Dellali, M., El Bour, M., Aissa, P. and Mahmoudi, E. (2010) The use of Fulvia fragilis (Mollusca: Cardiidae) in the biomonitoring of Bizerta lagoon: A multimarkers approach. Ecological Indicators, 10: 696-701.
McCormack, G. C., Powell, R and Keegan, B. (2000) Comparative analysis of two populations of the brittle star Amphiura filiformis (Echinodermata: Ophiuroidae) with different life history strategies using RAPD markers. Marine Biotechnology, 2: 100-106.
Muchmore, M.E., Moy, G.W., Swanson, W.J. and Vacquier, V.D. (1998) Direct sequencing of genomic DNA for characterization of a satellite DNA in five species of Eastern Pacific abalone. Molecular Marine Biology and Biotechnology, 7(1): 1-6.
Park, S.Y., Park, J.S. and Yoon, J.M. (2005) Genetic difference and variation in slipper lobster (Ibacus ciliatus) and deep sea lobster (Puerulus sewelli) throughout its distribution range determined by RAPD-PCR Analysis. Korean Journal of Genetics, 27: 307-317, 2005.
Tassanakajon, A., Pongsomboon, S., Jarayabhand, P., Klinbunga, S. and Boonsaeng, V. (1998) Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA analysis. Journal of Marine Biotechnology, 6: 249-254.
Yang, H.S. (2006) Reproductive cycle and proximate tissue composition of the cockle, Fulvia mutica from Anmyeon Island, on the west coast of Korea. Master thesis pp1-37.
Yoon, J.M. and Kim, Y.H. (2003a) Wide marsh clam (Corbicula spp.) populations from three sites analysed by RAPD-PCR-AGE. Bulletin of Electrochemistry, 19: 337-348.
Yoon, J.M. and Kim, G.W. (2003b) Genetic differences between cultured and wild penaeid shrimp (Penaeus chinensis) populations analysed by RAPD-PCR. Korean Journal of Genetics, 25: 21-32.
Yoon, J.M. and Kim, J.Y. (2004) Genetic differences within and between populations of Korean catfish (S. asotus) and bullhead (P. fulvidraco) analyzed by RAPD-PCR. Asian-Australasian Journal of Animal Sciences, 17: 1053-1061.
Yoke-Kqueen, C. and Radu, S. (2006) Random amplified polymorphic DNA analysis of genetically modified organisms. Journal of Biotechnology, 127: 161-166.