ISSN : 1225-3480
The morphological relations and physiological characteristics of the triploid and diploid oysters (Crassostrea gigas) in Taean area, west coast of Korea, were investigated from May 2012 to April 2013. Mophometric analysis indicated that the triploid oysters have the same shell length to shell height ratio but higher shell depth to shell height ratio than diploids. Consistent with morphological characteristics, triploid oysters showed greater values of fatness, condition index and RNA/DNA ratio during the period of experiment. The DNA concentration in adductor muscle and mantle of triploid were either lower or equal to the nucleic acids of diploid. However, RNA/DNA ratio were significantly higher than diploid. It appears that RNA/DNA ratio could be a useful indicator of health condition of triploid and diploid oysters when taken in correlation with the morphological indices.
Akashige, S. and Fushimi, T. (1992). Growth, survival and glycogen content of triploid Pacific oyster Crassostrea gigas in the waters of Hiroshima, Japan. Nippon Suisan Gakkaishi, 58: 1063-1071.
Allen Jr., S.K. (1983). Flow cytometry: Assaying experimental polyploid fish and shellfish. Aquaculture, 33:317-328.
Allen Jr., S.K. and Downing, S.L. (1986). Performance of triploid pacific oysters, Crassostrea gigas (Thunberg). I. Survival, growth, glycogen content and sexual maturation in yearlings. Journal of Experimental Marine Biology and Ecology, 102: 197-208.
Benfey, T.J. and Sutterlin, A.M. (1984). The haematology of triploid landlocked Atlantic salmon Salmo salar L. Journal of Fish Biology, 24: 333-338.
Evseev, G.A., Yakovlev, Y.M. and Li, X. (1996). The anatomy of the Pacific oyster, Crassostrea gigas (Thunberg) (Bivalvia: Ostreidea), Publications of the Seto Marine Biological Laboratory, 37:239-255.
Chation, J.A. and Allen Jr, S.K. (1985). Early detection of triploidy in the larvae of Pacific oysters, Crassostrea gigas, by flow cytometry. Aquaculture, 48: 35-43.
Hawkins, A.J.S., Day, A.J., Gerard, A., Naciri, Y., Ledu, C., Bayne, B.L. and Heral, M. (1994). A genetic and metabolic basis for faster growth among triploids induced by blocking meiosis I but not meiosis II in the larviparous european flat oyster, Ostrea edulis L., Journal of Experimental Marine Biology and Ecology, 184: 21-40
Hur, Y.B., Min, K.S. Kim, T.E. Lee, S.J. and Hur, S.B. (2008) Larvae growth and biochemical composition change of the Pacific oyster, Crassostrea gigas, larvae during artificial seed production. Journal of Aquaculture, 21(4): 203-212 (in Korean).
Frankauser, G. (1941). Cell size, organ and body size in triploid newts (Triturus viridescens). Journal of Morphology, 68: 161-177.
Fankhauser, G. (1945). The effects of changes in chromosome number on amphibian development. Quarterly Review of Biology, 20: 20-78.
Garnier-Gere, P.H., Naciri-Graven, Y., Bougrier, Heral, M., Kotoulas, G., Haqwkins, A., and Gerard, A. (2002). Influences of triploidy, parentage and genetic diversity on growth of the Pacific oyster Crassostrea gigas reared in contrasting natural environments. Molecular Ecology, 11: 1499-1514.
Greenlee, A.R., Kersten, C.A. and Cloud, J.G. (1995). Effects of triploidy on rainbow trout myogenesis in vitro. Journal of Fish Biology, 46: 381-388.
Kim, S.K., Rosenthal, H., Clemmesen, C., Park, K.Y, Kim, D.H., Choi, Y.S. and Seo, H.C., (2005) Various methods to determine the gonadal development and spawning season of the purplish washington clam, Saxidomus purpuratus (Sowerby). Journal of applied lchthyology, 21: 101-106.
Kim, S.K., Choi, E.H., Han, H.S. and Lim, H.J. (2012). Ecophysiological characteristics changes in the Pacific oyster, Crassostrea gigas, after spawning season in off-bottom culture. Korean Journal of Malacology, 28(3): 215-223.
Li, Y., Qin, J.G., Li, X. and Benkendorff, K. (2009). Monthly variation of condition index, energy reserves and antibacterial activity in Pacific oysters, Crassostrea gigas, in Stansbury (South Australia). Aquaculture, 286: 64-71.
Lim, H.J., Lee, T.S., Cho, P.G., Back, S.H., Byun, S.G. and Choi, E.H. (2011). The production efficiency of cupped oyster, Crassostrea gigas Spat according to clutch aqnd growth comparing diploid and triploid oysters in off-bottom culture for tidal flat utilization. Korean Society of Fisheries and Aquatic Science, 44(3): 259-266.
Maguire, G.B., Boocock, B., Kent, G.N., Gardner, N.C. (1994). Studies on triploid oysters in Australia: IV. Sensory evaluation of triploid and diploid Pacific oysters, Crassostrea gigas (Thunberg), in Tasmania. In: Nell, J.A., Maguire, G.B. (Eds.), Evaluation of triploid Sydney Rock Oysters (Saccostrea commercialis) and Pacific Oysters (Crassostrea gigas) on Commercial Leases in New South Wales and Tasmania, Final Report to FRDC, September, 1994, NSW Fisheries, Port Stephens Research Centre, Taylors Beach, NSW and University of Tasmania, Launceston, Tas., pp. 178-193.
Mugnier, C., Justou, C., Lemonnier, H., Patrois, J., Ansquer, D., Goarant, C. and Lecoz, J.R. (2013). Biological, physiological, immunological and nutritional assessment of farm-reared Litopenaeus stylirostris shrimp affected or unaffected by vibriosis. Aquaculture, 388-391: 105-114.
Nell, J.A. (2002). Farming triploid oysters. Aquaculture, 210: 69-88.
Nell, J.A. and Perkins, B. (2005). Studies on triploid oysters in Australia: farming potential of all triploid Pacific oysters, Crassostrea gigas (Thunberg), in Port Stephens, New South Wales, Australia Aquaculture Research, 36: 530-536.
NFRDI (2012). Research for aquaculture productivity improvement of Pacific oyster, Crassostrea gigas in tidalflat. 2/2 Technical report of NFRDI, 607-624.
Ning, J., Li, C., Yang, G., Wan, A. and Sun, S. (2013). Use of RNA:DNA ratios to evaluate the condition and growth of the copepod Calanus sinicus in the southern Yellow Sea. Deep-Sea Research II. (in progress).
Small, S. A. and Benfey, T.J. (1987). Cell size in triploid salmon. Journal of Experimental Zoology, 241: 339-342.
Suresh, A.V. and Sheehan, R.J. (1998a). Muscle fiber growth dynamics in diploid and triploid rainbow trout, Oncorhynchus mykiss. Journal of Fish Biology, 52: 570-587.
Suresh, A.V. and Sheehan, R.J. (1998b). Biochemical and morphological correlates of growth in diploid and triploid rainbow trout. Journal of Fish Biology, 52: 588-599.
Swarup, H. (1959). Effect of triploidy on the body size, general organization and cellular structure in Gasterosteus aculeatus (L.). Journal of Genetics, 56: 143-155.
Yang H., Gallivan T., Guo X. and Allen S., 2000. A method for preserving oyster tissue samples for flow cytometry. Journal of shellfish Research, 19: 835-839.
Westerman, M. and Holt, G.J. (1994). RNA:DNA ratio during the critical period and early larval growth of the red drum Sciaenops ocellatus. Marine Biology, 121: 1-9.
Wright, D.A. and Hetzel, E.W. (1985). Use of RNA:DNA ratios as an indicator of nutritional stress in the American oyster Crassostrea virginica. Marine Ecological Progress Series, 25: 199-206.
Writgth, D.A. and Martin, F.D. (1985). The effect of starvation on RNA-DNA ratios and growth of larval striped bass, Morone saxatilis. Journal of Fish Biology, 27(4): 479-485.