ISSN : 1225-3480
고랑가리비의 산란을 유도하고 수정난의 발생과 유생의 성장과정을 조사하였다. 산란자극 방법으로 광 자극, 간출 자극,온도 자극, 그리고 간출 후 온도자극을 수온 16 ± 0.5℃에서각각 실시하였다. 광 자극은 암컷 1마리당 700-900천개, 온도자극은 700-800천개로 적은 산란량을 보인 반면 간출 자극은700-1,200천개로 높았으며 간출 자극 후 온도자극이1,000-1,500천개로 가장 높은 산란량을 보였다. 수정율에 있어 광 자극은 암컷 1마리당 71.7%, 온도자극은 73.4%, 간출자극이 73.6%이었으며 간출 자극 후 온도자극은 76.3%로 가장 높았으나 자극방법에 따른 수정율의 유의적인 차이는 없었다. 수정란부터 D상 유생으로의 수온별 발생과정을 조사하기위하여 비이커에 ml당 1,000개의 수정란을 수용하고 각각8℃, 12℃, 16℃, 20℃ 및 24℃에서 D상 유생으로 발생하는생존율을 광학현미경을 사용하여 30분 간격으로 관찰한 결과,8℃와 24℃의 실험군이 4.1%와 3.2%로 생존율이 낮은 편이었으며 16℃에서 32.7%로 가장 높은 생존율을 보여주었다. 수정란의 크기는 72 ± 2.1 μm, 담륜자 유생은 103 ± 3.8 μm,D상 유생은 129 ± 10.4 μm, 각정기 유생은 145 ± 16.8 μm, 후기 유생은 197 ± 13.6 μm이었으며 528시간 후에 각장245 ± 15.8 μm의 초기 부착종묘로 성장하였다.
The development of swift's scallop Chlamys swiftii, reared in the laboratory, has been examined through the investigation of morphological characteristics in fertilized egg, larvae and juvenile. Eggs were fertilized with a dilute sperm solution to improve the survival of fertilized eggs. Developing larvae were maintained at a temperature of 16 ± 0.5℃ and salinity concentration of 33 ppt. We have investigated the fertilization rates and egg number spawned at several stimulating conditions such as sunlight exposure, air dry, seawater temperature rise (5℃) and seawater temperature rise (5℃) after exposure of air dry for spawning induction of swift's scallop Chamys swiftii. Stimulation treated with sunlight exposure and seawater temperature rise (5℃) have shown the spawning number of 700,000-900,000 and 700,000-800,000 per individual, respectively while stimulation treated with seawater temperature rise (5℃) after exposure of air dry have shown the high spawning number of 1,000,000-1,500,000 per individual. Survival rate of D-shaped larvae of swift's scallop put into the different seawater temperatures of 8℃, 12℃, 16℃, 20℃ and 24℃ has been 4.1%, 11.6%, 32.7%, 18.6% and 3.2%, respectively. Fertilized eggs with the diameter of 72 μm developed into trochophore larvae of 103 ± 3.8 μm shortly after 35 hours and to D larvae of 129 ± 10.4 μm shell length within 72 hours. It took 336 hours to become initial Umbo-stage larva of 145 ± 16.8 μm shell length. Post larvae, which have been 197 ± 13.6 μm shell length, spontaneously have settled in the attachment substances. It have required 528 hours from fertilized eggs to early attached juvenile to become initial juvenile size of 245 ± 15.8 μm shell length.
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