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ISSN : 1225-3480
본 연구는 <TEX>${\beta}$</TEX>-glucan이 첨가된 사료첨가제 (이뮨글루<TEX>$^{TM}$</TEX>) 급이시 전복의 비특이 면역력의 증강 효과와 전염성 질병 감염 시 생존율의 변화를 확인하고자 수행되었다. 이를 위하여 참전복 치패와 성패를 대상으로 이뮨글루<TEX>$^{TM}$</TEX> 0, 0.1, 1%가 함유된 사료를 14일간 급이 하면서 면역력, 정균력, 생존율 등을 조사하였다. 조사 결과 이뮨글루<TEX>$^{TM}$</TEX>가 함유된 사료를 급이한 전복의 치패와 성패의 식세포작용이 증가하였으며 전복폐사 원인균인 Vibrio alginolyticus에 대한 정균력과 생존율이 상승하였다. 반면 염증량을 나타내는 NO량 역시 증가하였다. 따라서 <TEX>${\beta}$</TEX>-glucan이 첨가된 사료첨가제 이뮨글루<TEX>$^{TM}$</TEX>는 전복 사육 시 전복의 면역력을 높이고 폐사율을 낮추는데 유용한 것으로 판단된다.
The present study aimed to understand the effects of a ß-glucan-added abalone feed (Immuneglu™) on the immune parameters, survival rate, and anti-bacterial activity of the abalone Nordotis discus hannai. During the study, spat and adult abalones were fed 0, 0.1, and 1% of Immuneglu™ mixed with artificial feed for 2 weeks, and their immune parameters, survival rate, and anti-bacterial activity were measured. The results showed that abalones fed on Immuneglu™-added feed showed a higher phagocytic rate, survival rate, and anti-bacterial activity against Vibrio alginolyticus, than those with the control treatment. In addition, the nitric oxide concentration, which indicates an inflammatory response, increased in the treatment group. Our study suggests that Immuneglu™ might be a useful additive for increasing the immunity and survival rates of abalones during mass culture.
Ainsworth, A.J. (1994) A β-glucan inhibitable zymosan receptor on channel catfish neutrophils. Veterinary Immunology and Immunopathology, 41: 141-152.
Aderem, A. and Ulevitch, R.J. (2000) Toll-like receptors in the induction of the innate immune response. Nature, 406: 782-787.
Bower, S.M. (2011) Synopsis of Infectious Diseases and Parasites of Commercially Exploited Shellfish: Table of Contents. http://www.dfo-mpo.gc.ca/science/aah-saa/diseases-maladies/toc-eng.html#aba.
Brogden, G., Krimmling, T., Adame, K.M., Naim, H.Y., Steinhagen, D. and von Kockritz-Blickwede, M. (2014) The effect of b-glucan on formation and functionality of neutrophil extracellular traps in carp (Cyprinus carpio L.). Developmental and Comparative Immunology, 44: 280-285.
Costa, M.M., Novoa, B. and Figueras, A. (2008)Influence of β-glucans on the immune responses of carpet shell clam (Ruditapes decussatus) and Mediterranean mussel (Mytilus galloprovincialis). Fish & Shellfish Immunology, 24(5): 498-505.
Diao, J., Ye, H.B., Yu, X.Q., Fan, Y., Xu, L., Li T.B., Wang Y.Q.(2013) Adjuvant and immunostimulatory effects of LPS and β-glucan on immune response in Japanese flounder, Paralichthys olivaceus. Veterinary Immunology and Immunopathology, 156: 167-175.
Ghaedi, G., Keyvanshokooh, S., Azarm, H.M. and Akhlaghi, M. (2015) Effects of dietary β-glucan on maternal immunity and fry quality of rainbow trout (Oncorhynchus mykiss). Aquaculture, 441: 78-83.
KIPO. (2016) Korea Intellectual Property Rights Information Service. http://www.kipris.or.kr/khome/main.jsp.
KNSO (2015) Korea National Statistical Office. Fishery Production Survey DB, Daejeon, Korea. http://kosis.kr/statisticsList/statisticsList_01List.jsp?vwcd=MT_ZTITL E&parentId=F.
Liu, P.C., Chen Y.C., and Lee, k.k. (2001) Pathogenicity of Vibrio alginolyticus isolated from diseased small abalone Haliotis diversicolor supertexta. Microbiosis, 104: 71-7.
Medzhitov, R. and Janeway, Jr. C.A. (1997) Innate immunity: the virtues of a nonclonal system of recognition. Cell, 91: 295-298.
NFRDI (1998) Seed production and grow out culture of abalone. Fisheries Technology of National Fisheries Research and Development Institute, 3: 30-43 [In Korean].
Nam, K.W., Yang, H.S., and Park, K.I. (2013)Quantification of nitric oxide concentration in the hemocytes of Manila clam. Korean Journal of Malacology, 29: 15-21. [In Korean with English abstract]
Nam, K.W. and Park, K.I. (2015) Effect of β-glucan on immune parameters in the Manila clam Ruditapes philippinarum. The Korean Journal of Malacology, 31(2): 123-127. [In Korean with English abstract]
Park, K.I, Park, H.S., K, J.M., Park, Y.J., Hong, J.S., and Choi, K.S. (2006) Flow cytometeric assessment of immnune parameters of the manila clam (Ruditapes philippinarum). Journal of the Korean Fisheries Society, pp. 123-131. [In Korean with English abstract]
Park, M.A (2012) Current status of diseases and prevention technique for abalone in Korea and other countries. http://www.nifs.go.kr/fdcc/htm/disease03_02.jsp [In Korean]
Park, S.O., and Kim, J. (2012) Functional food for immune regulation - beta-glucan. Food Science and Industry, 45: 39-47.
Rock, K.L., and Kono, H. (2008) The inflammatory response to cell death. Annual Review of Pathology, 3: 99–126.
Sirimanapong, W., Adams, A., Ooi E.L., Green, M.D., Nguyen, D.K., Browdy, L,C., Collet, B. and Kim, D.T. (2015) The effects of feeding immunostimulant β-glucan on the immune response of Pangasianodon hypophthalmus. Fish & Shellfish Immunology, 45:357-366.
Wongsasak, U., Chaijamrus, S., Kumkhong, S. and Boonanuntanasarn, S. (2015) Effects of dietary supplementation with β-glucan and synbiotics on immune gene expression and immune parameters under ammonia stress in Pacific white shrimp. Aquaculture, 436: 179-187.
Wu, Y.S., Tseng, T.Y., and Nan, F.H. (2016)Beta-1,3-1,6-glucan modulate the non-specific immune response to enhance the survival in the Vibrio alginolyticus infection of Taiwan abalone (Haliotis diversicolor supertexta). Fish & Shellfish Immunology, 54: 556-563.
Zhao, Y., Ma, H., Zhang, W., Ai, Q., Mai, K., Xu, Wang, X. and Liufu, Z. (2011) Effects of dietary β-glucan on the growth, immune responses and resistance of sea cucumber, Apostichopus japonicus against Vibrio splendidus infection, Aquaculture, 315(3): 269-274