open access
메뉴
open access
메뉴
ISSN : 1225-3480
CO-I서열의 생물정보학적 분석 결과 국내에서 유통되는 재첩류의 연체부는 일본재첩 (C. japonica) 과 재첩 (C. fluminea) 으로 동정되어 두 종이 혼재하여 유통되는 것으로 확인되었다. 일본재첩 (C. japonica) 과 재첩 (C. fluminea) 은 국내에서 서식하고 있으나, 섬진강 유역의 재첩 (C. fluminea) 은 개체수가 감소하여 거의 생산되지 않고 있으므로, 본 연구를 통해 확인된 재첩 (C. fluminea) 은 중국 수입산으로 예상된다.
The natives of the genus Corbicula have shown worldwide dispersion in recent times, which has caused great ecological and economic impacts on the introduced ecosystems. The species reported from the genus have been consumed as food and explored for medicine with pharmacological activity. Consequently, the demand of Corbicula sp. in the South Korean domestic market has increased and so also it’s associated import to the country. However, due to the absence of identification keys of imported Corbicula, the market is facing confronting situations. We hypothesized that the mitochondrial Cytochrome Oxidase I gene (CO-I) based molecular profiling could be a necessary technique for identification of Corbicula sp. in the South Korea domestic market. The genetic analysis identified both Corbicula japonica and Corbicula fluminea from the market foods. C. japonica and C. fluminea are inhabitants in Korea, but C. fluminea production has decreased in Seomjingang river basin. Therefore, C. fluminea identified from this study, is expected to be imported from China and would have a mixed sales in Seomjingang river side basin.
Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. (1990) Basic local alignment search tool. Journal of molecular biology, 215: 403-410.
Bang, I.S., and Lee, Y.S. (2014) Molecular Phylogenetic Study of Nesiohelix samarangae Based on CO-I Gene. The Korean Journal of Malacology, 30:391-397.
Chung, P.-., Park, G.-., Jung, Y., Yong, T.-., Im, K.-., and Soh, C.-. (2000) Mdicinal Mollusks in Korea. The Korean Journal of Malacology, 16: 55-60.
Ewing, B., and Green, P. (1998) Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome. Res., 8: 186-194.
Ewing, B., Hillier, L., Wendl, M.C., and Green, P. (1998)Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome. Res., 8:175-185.
Hebert, P.D.N., Ratnasingham, S., and de Waard, J.R. (2003) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society of London B:Biological Sciences, 270: S96-S99.
Hee Ju, H., Se Won, K., So Young, P., Jong Min, C., Dae Kwon, S., Hyeongchun, P., Hong Seog, P., Yeon Soo, H., Jun-Sang, L., and Yong Seok, L. (2016)Classification and Phylogenetic Studies of Cephalopods from four countries of South-East Asia. The Korean Journal of Malacology, 32: 55-62.
Higgins, D.G., and Sharp, P.M. (1988) CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene, 73: 237-244.
Huang, X., and Madan, A. (1999) CAP3: A DNA sequence assembly program. Genome. Res., 9:868-877.
Kang, S.W., Hwang, H.J., Park, S.Y., Wang, T.H., Park, E.B., Lee, T.H., Hwang, U.W., Lee, J.-S., Park, H.S., Han, Y.S., Lim, C.E., Kim, S., and Lee, Y.S. (2014)Mollusks Sequence Database: Version II. The Korean Journal of Malacology, 30: 429-431.
Kang, S.W., Park, S.Y., Patnaik, B.B., Hwang, H.J., Kim, C., Kim, S., Lee, J.S., Han, Y.S., and Lee, Y.S. (2015) Construction of PANM Database (Protostome DB) for rapid annotation of NGS data in Mollusks. The Korean Journal of Malacology, 31: 243-247.
Kim, W.-K., Lee, C.-S., Lee, J.-Y., and Hu, S.-B. (2002)Production of Artificial Seedling of the Brackish Water Clam, Corbicula japonica. Journal of Aquaculture, 15: 23-29.
Kress, W.J., García-Robledo, C., Uriarte, M., and Erickson, D.L. (2015) DNA barcodes for ecology, evolution, and conservation. Trends in Ecology &Evolution, 30: 25-35.
Layton, K.K.S., Martel, A.L., and Hebert, P.D.N. (2014)Patterns of DNA Barcode Variation in Canadian Marine Molluscs. PLoS. ONE, 9: e95003.
Lee, J.-Y., Kim, W.-k., and Lee, C.-s. (2011) Growth and survival of the brackish water clam, Corbicula japonica larvae according to rearing conditions. The Korean Journal of Malacology, 27: 337-343.
Lee, J.B., Shin, Y.J., Lee, J.H., Choi, Y.M., Lee, D.W., and Cha, H.K. (2012) Estimation of potential fishery yield for Corbicula japonica in the Seomjin River, Korea. The Korean Journal of Malacology, 28: 91-98.
Lee, J.S. (2015) National list of species of the Korea [Invertebrates-VI] pp. National Institute of Biological Resources.
Lee, Y.S., Jo, Y.-H., Kim, D.-S., Kim, D.-W., Kim, M.-Y., Choi, S.-H., Yon, J.-O., Byun, I.-S., Kang, B.-R., Jeong, K.-H., and Park, H.-S. (2004) Construction of BLAST Server for Mollusks. Korean Journal of Malacology, 20: 165-169.
Musto, M. (2011) DNA quality and integrity of nuclear and mitochondrial sequences from beef meat as affected by different cooking methods. Food Technology and Biotechnology, 49: 523-528.
Park, J.-K., and Kim, W. (2003) Two Corbicula (Corbiculidae: Bivalvia) mitochondrial lineages are widely distributed in Asian freshwater environment. Molecular Phylogenetics and Evolution, 29: 529-539.
Ryu, D.K., Chung, E.-Y., and Kim, Y.H. (2005) Age and Growth of the Brackish Water Clam, Corbicula japonica Prime on the West Coast of Korea. The Korean Journal of Malacology, 21: 57-64.
Ministry of Food and Drug Safety (2015) Status of importation for fishery product
Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30: 2725-2729.