ISSN : 1225-3480
굴의 종패생산 시 수정란을 대량으로 얻기 위하여 성숙한 굴 어미를 대상으로 신경전달 물질인 serotonin의 최적 처리 농도를 파악하기 위한 연구가 수행되었다. Serotonin 주사 후 방란까지 소요되는 시간은 7.6분부터 27분까지 였으며, 농도가 낮아짐에 따라 소요시간은 유의하게 길어졌다 (P < 0.05). 방정시간 역시 방란시간과 비슷한 경향을 보였다. Serotonin 농도에 따른 반응률 역시 처리 농도가 높을수록 반응률이 높아졌다. Serotonin 처리 후 산란량을 조사한 결과, <TEX>$10^{-2}M$</TEX> 에서 2,664천개로 <TEX>$10^{-5}M$</TEX> 농도에 비해 유의하게 많았으나 (P < 0.05), <TEX>$10^{-4}$</TEX>나 <TEX>$10^{-3}M$</TEX> 처리구는 유의한 차이를 나타내지 않았다 (P > 0.05). Serotonin 처리 후 산란된 알의 GVBD율, 수정률 및 부화율은 농도에 영향을 받지 않았다.
In order to obtain a large number of fertilized eggs for seedling production, experiment was carried out examine effects of serotonin on spawning of the Pacific oyster Crassostrea gigas. The shorter response time to initial spawning in case of serotonin injection showed, the higher serotonin injected with 7.6-27 min. The response time to initial sperm releasing showed the same tendency with female. The highest response rates and eggs amount spawned were showed in the highest concentration. The serotonin injection had no effect on frequency of germinal vesicle breakdown (GVBD), fertilization and hatching rate.
Alcazar, S.N., Solis, E.P., and Alcala, A.C. (1987)Serotonin-induced spawning and larval rearing of the China clam, Hippopus porcellanus rosewater (Bivalvia: Tridacnidae). Aquaculture, 66: 359-368.
Braley, R.D. 1985. Serotonin-induced spawning in giant clams (Bivalvia: Tridacnidae). Aquaculture, 47:321-325.
Chang, Y.J. (2015) Overview of Physio-Aquaculture. pp. 93-105. Aqua Info Company, Seoul. [in Korean]
Fong, P.P., Wall, D.M., and Ram, J.L. (1993)Characterization of serotonin receptors in the regulation of spawning in the zebra mussel Dreissena polymorpha (Pallas). J. Exp. Zool, 267:475-482.
Fong, P.P., Duncan, J., and Ram, J.L. (1994) Inhibition and sex specific induction of spawning by serotonergic ligands in the zebra mussel Dreissena polymorpha (Pallas). Experientia, 50: 506-509.
Fong, P.P., Deguchi, R., and Kyozuka, K. (1996)Serotonergic ligands induce spawning but not oocyte maturation in the bivalve Mactra chinesis from central J. Biol. Bull., 191: 27-32.
Galtsoff, P. S. (1938). Physiology of reproduction of Ostrea virginica I. Spawning reaction of the female and male. Biol. Bull. 74: 461.
Gibbons, M.C., and Castagna, M. (1984) Serotonin as an inducer of spawning in six bivalve species. Aquaculture, 40: 189-191.
Kang, K.H., Baik, K.K., Chang, Y.J., and Yoo, S.K. (1996) Spawning induction according to stimulating treatment and spat rearing of scallop, Patinopecten yessoensis. Kor. J. Malacol., 12: 99-104. [in Korean]
Kikuchi, S., and Uki. N. (1974) Technical study on artificial spawning of abalone, genus Haliotis Ⅰ. Relation between water temperature and advancing sexual maturity of Haliotis discus hannai. Bull. Tohoku Reg. Fish. Res. Lab, 33: 69-78.
Lee, J.Y. (2001) Reproductive cycle and seedling production of surf clam, Spisula sachalinensis. Ph.D. thesis, pp. 142. Pukyong National University, Korea. [in Korean]
Lee, J.Y., Chang, Y.J., and Park, Y.J. (1996) Spawning induction and egg development of surf clam, Spisula sachalinensis. J. Aquacult., 9: 419-427. [in Korean]
Lee, J.Y., Park, Y.J., and Chang, Y.J. (1999). Gonadal development reproductive cycle of Gomphina melanaegis (Bivalve; Veneridae). J. Korean Fish. Soc., 32: 198-203. [in Korean]
Loosanoff, V.L., and Davis, H.C. (1963) Rearing of bivalve molluscs. Advanced Marine Biology, 1: 1-136.
Matsutani, T., and Nomura, T. (1982) Induction of spawning by serotonin in the scallop Patinopecten yessoensis (Jay). Mar. Biol. Lett., 3: 353-358.
O’Connor, W.A., and Heasman, M.P. (1995) Spawning induction and fertilisation in the doughboy scallop Chlamys (Mimachlamys) asperrima. Aquaculture, 136:117-129.
Osada, M., Mori, K., and Nomura, T. (1992). In vitro effects of estrogen and serotonin on release of eggs from the ovary of the scallop. Nippon Suisan Gakkaishi., 58: 223-227.
Park, K.Y., Kim, S.K., Seo, H.C., and Ma, C.W. (2005)Spawning and larval development of the jicon scallop, Chlamys farreri. J. Aquacult., 18: 1-6. [in Korean]
Stephano, J.L., and Gould, M. (1988). Avoiding polyspermy in the oyster (Crassostrea gigas). Aquaculture, 73: 295-307
Velasco, L.A., Barros, J., and Acosta, E. (2007) Spawning induction and early development of the Caribbean scallops Argopecten nucleus and Nodipecten nodosus. Aquaculture, 266: 153-165.
Vélez, A., Alifa, E., and Aguaje, O. (1990) Induction of spawning by temperature and serotonin in the hermaphroditic scallop Pecten ziczac. Aquaculture, 84:307-313.
Yoo, S.K., and Kang, K.H. (1996) Spawning induction according to stimulating treatment and influence of water temperature on egg development and larvae rearing of oyster, Crassostrea gigas. Korean J. Malacol., 12: 91-97. [in Korean]
