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ISSN : 1225-3480
굴의 종패생산 시 수정란을 대량으로 얻기 위하여 성숙한 굴 어미를 대상으로 신경전달 물질인 serotonin의 최적 처리 농도를 파악하기 위한 연구가 수행되었다. Serotonin 주사 후 방란까지 소요되는 시간은 7.6분부터 27분까지 였으며, 농도가 낮아짐에 따라 소요시간은 유의하게 길어졌다 (P < 0.05). 방정시간 역시 방란시간과 비슷한 경향을 보였다. Serotonin 농도에 따른 반응률 역시 처리 농도가 높을수록 반응률이 높아졌다. Serotonin 처리 후 산란량을 조사한 결과, <TEX>$10^{-2}M$</TEX> 에서 2,664천개로 <TEX>$10^{-5}M$</TEX> 농도에 비해 유의하게 많았으나 (P < 0.05), <TEX>$10^{-4}$</TEX>나 <TEX>$10^{-3}M$</TEX> 처리구는 유의한 차이를 나타내지 않았다 (P > 0.05). Serotonin 처리 후 산란된 알의 GVBD율, 수정률 및 부화율은 농도에 영향을 받지 않았다.
In order to obtain a large number of fertilized eggs for seedling production, experiment was carried out examine effects of serotonin on spawning of the Pacific oyster Crassostrea gigas. The shorter response time to initial spawning in case of serotonin injection showed, the higher serotonin injected with 7.6-27 min. The response time to initial sperm releasing showed the same tendency with female. The highest response rates and eggs amount spawned were showed in the highest concentration. The serotonin injection had no effect on frequency of germinal vesicle breakdown (GVBD), fertilization and hatching rate.
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