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ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

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공기노출 및 절식시기 동안 참담치, Mytilus coruscus 에서 Hsp70 및 GST 유전자 발현에 대한 연구

The Expression of Hsp70 and GST Genes in Mytilus coruscus during Air Exposure and Starvation

Abstract

Heat shock proteins (HSPs), one of the most highly conserved groups of proteins characterized to date, play crucial roles in protecting cells against environmental stresses, such as heat shock, salinity and oxidative stress. The glutathione S-transferases (GST) have important role in detoxification of oxidative stress, environmental chemicals and environmental stress. GST mRNA expression have been used as biomarkers on environmental stress. The purpose of this study was to investigate the death rate and the gene expression of Hsp70 and GST during air exposure and starvation. Results showed that, the expression of Hsp70 mRNA was significantly changed in the experiment groups, such as air exposure and starvation. GST mRNA expression was significantly increased in the experimental group of starvation. These results suggest that Hsp70 and GST were played roles in biomarker gene on the air exposure and starvation.

keywords
Mytilus coruscus, Hsp70, GST, air exposure, tarvation

참고문헌

1.

Anestis, A., Portner, H.O. and Michaelidis, B. (2010)Anaerobic metabolic patterns related to stress responses in hypoxia exposed mussels Mytilus galloprovincialis. Journal of experimental marine biology and ecology, 394: 123-133.

2.

Antonopoulou, E., Kentepozidou, E., Feidantsis, K., Roufidou, C., Despoti, S. and Chatzifotis, S. (2013) Starvation and re-feeding affect Hsp expression, MAPK activation and antioxidant enzymes activity of European Sea Bass (Dicentrarchus labrax). Comparative biochemistry and physiology. A, 165: 79-88.

3.

Ashton-Alcox, K.A. and Ford, S.E. (1998) Variability in molluscan hemocytes: a flow cytometric study. Tissue and Cell, 30: 195-204.

4.

Bailey, J., Parsons, J. and Couturier, C.A. (1996)Salinity tolerance in the blue mussel, Mytilus edulis. Bull. Aquacult. Assoc. Can., 96: 74-76.

5.

Bukau, B. and Horwich, A.L. (1998) The Hsp70 and Hsp60 chaperone machines. Cell, 92: 351-366.

6.

Chapple, J.P., Smerdon, G.R., Berry, R.J. and Hawkins, A.J.S. (1998) Seasonal changes in stress-70 protein levels reflect thermal tolerance in the marine bivalve Mytilus edulis L. J. Exp. Mar. Biol. Ecol., 229: 53-68.

7.

Chirico, W.J., Waters, M.G. and Blobel, G. (1988) 70K heat shock related proteins stimulate protein translocation into microsomes. Nature, 332: 805-810.

8.

Clark, A.G. (1989) The comparative enzymology of the glutathione S-transferases from non-vertebrate organisms. Comp. Biochem. Physiol., 92: 419-446.

9.

Colinet, H., Lee, S.F. and Hoffmann, A. (2010) Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster. FEBS J., 277: 174-185.

10.

Dabrowski, K.R. (1986) Active metabolism in larval and juvenile fish: ontogenetic changes, effect of water temperature and fasting. Fish Physiology and Biochemistry, 1: 125-144.

11.

Davenport, J. and Wong, T.M. (1986) Responses of the blood cockle Anadara granosa (L) (Bivalvia: Arcidae)to salinity, hypoxia and aerial exposure. Aquaculture, 56: 151-162.

12.

Deaton, L.E., Derby, J.G.B., Subhedar, N. and Greenberg, M. (1989) Os-moregulation and salinity tolerance in two species of bivalve mollusc: Limnoperna fortunei and Mytilopsis leucophaeta. J. Exp. Mar. Ecol., 133: 67-79.

13.

Dong, Y.W., Dong, S.L. and Ji, T.T. (2008) Effects of different thermal regimes on growth and physiological performance of the sea cucumber Apostichopus japonicus Selenka. Aquaculture, 275:329-334.

14.

Fangue, N.A., Hofmeister, M. and Schulte, P.M. (2006)Intraspecific variation in thermal tolerance and heat shock protein gene expression in common killifish, Fundulus heteroclitus. J. Exp. Biol., 209: 2859-2872.

15.

Feder, M.E. and Hofmann, G.E. (1999) Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology. Annu. Rev. Physiol., 61: 243-282.

16.

Fry, F.E.J. (1971) The effects of the environmental factors on the physiology of fish. In: Fish Physiology, Vol. 6. (ed. by Hoar WS and Randall DJ), pp. 1-98. Academic Press, New york.

17.

Gagnaire, B., Heloise, F., Kebin, M., Helene, T.G. and Tristan, R. (2006) Effects of temperature and salinity on hemocyte activities of the Pacific oyster, Crassostrea gigas (Thunberg). Fish & Shellfish Immunology, 20: 536-547.

18.

Galea-Lauri, J., Richardson, A.J., Latchman, D.S. and Katz, D.R. (1996) Increased heat shock protein 90 (hsp90) expression leads to increased apoptosis in the monoblastoid cell line U937 following induction with TNF-alpha and cycloheximide: a possible role in immunopathology. J. Immunol., 157: 4109-4118.

19.

Georgopoulos, C. and Welch, W. (1993) Role of the major heat shock proteins as molecular chaperones. Annu. Rev. Cell Biol., 9: 601-634.

20.

Hamed, R.R., Farid, N.M., Elowa, S.E. and Abdalla, A.M. (2003) Glutathione related enzyme levels of freshwater fish as bioindicators of pollution. The Environmentalist, 23: 313-322.

21.

Hansson, T., Schiedek, D., Lehtonen, K.K., Vuorinen, P., Liewenborg, J., Noaksson, E., Tjarnlund, U., Hansson, M. and Balk, L. (2006) Biochemical biomarkers in adult female perch (Perca fluviatilis) in a chronically polluted gradient in the Stockholm recipient (Sweden). Marine Pollution Bulletin, 53: 451-468.

22.

Hayes, J.D. and Pulford, D.J. (1995) The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemprotection and drug resistance. Critical Reviews in Biochemistry and Molecular Biology, 30: 445-600.

23.

Hayes, J.D., Flanagan, J.U. and Jowsey, I.R. (2005)Glutathione transferases. Annual Review of Pharmacology and Toxicology, 45: 51-88.

24.

Hur, Y.B., Min, K.S., Kim, T.E., LEE, S.J. and Hur, S.B. (2008) Larvae growth and biochemical composition change of the Pacific Oyster Crassostrea gigas, Larvae during artificial seed production. J. Aquaculture, 21: 203-212.

25.

Hur, Y.B., Kim, T.E., Lee, S.J. and Hur, S.B. (2010)Variations in Reserved Nutrient Consumption and Growth of Pacific Oyster (Crassostra gigas) Larvae during Starvation. Korean J. Fish. Aquat. Sci., 43(5):489-494.

26.

Imai, J. and Yahara, I. (2000) Role of HSP90 in salt stress tolerance via stabilization and regulation of calcineurin. Mol. Cell Biol., 20: 9262-9270.

27.

Jakob, U., Lilie, H., Meyer, I. and Buchner, J. (1995)Transient interaction of Hsp90 with early unfolding intermediates of citrate synthase. J. Biol. Chem., 270: 7288-7294.

28.

Kim, C.W., Jin, Y.G., Kim, T.I., Jeong, D.S. and Kang, H.S. (2015) The expression of Hsp90 and ferritin genes under thermal stress in the sea cucumber (Apostichopus japonicas). Korean J. Environ. Biol., 33: 433-440.

29.

Kim, T.H., Kim, K.J., Choi, M.K. and Yeo, I.K. (2006)Physiological changes of juvenile abalone, Haliotis sieboldii exposed to acute water-temperature stress. J. of Aquaculture, 77-83.

30.

Kregel, K.C. (2002) Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance. J. Appl. Physiol., 92 :2177-2186.

31.

Lee, G., Hwang, J., Chung, Y., Kim, D., Moh, S.H., Chang, M. and Lee, T.K. (2012) Effects of hypoxia on the fertilization and early development of sea urchin, Strongylocentrous nudus. Journal of the Korea Academia-Industrial cooperation Society, 13:3785-3791.

32.

Li, P., Zha, J. and Zhou, K.Y. (2009) Molecular cloning, mRNA expression, and characterization of HSP90gene from Chinese mitten crab Eriocheir japonica sinensis. Comp. Biochem. Physiol. B, 153: 229-235.

33.

Liu, H., He, J., Chi, C. and Shao, J. (2014) Differential HSP70 expression in Mytilus coruscus under various stressors. Gene, 543: 166-173.

34.

Looise Bas, A.S., Holwerda, D.A. and Foekema, E.M. (1996) Induction of glutathione S-transferase in the freshwater bivalve Sphaerium corneum as a biomarker for short-term toxicity tests? Comp. Biochem. Physiol. C, 113: 103-107.

35.

Loomis, S.H., Ansell, A.D., Gibson, R.N. and Barnes, M. (1995) Freezing tolerance of marine invertebrates. Oceanogr. Mar. Biol. Annu. Rev., 33: 337-350.

36.

Maslin, J.L. (1989) The salinity tolerance of Corbula trigona (Bivalvia: Corbulidae) from a West-African lagoon and its variations. Arch. hydrobiol., 117:205-223.

37.

Nam, B.H., Park, E.M., Kim, Y.O., Kim, D.G., Jee, Y.J., Lee, S.J. and An, C.M. (2013) Analysis of heat, cold or salinity stress-inducible genes in the Pacific abalone, Haliotis discus hannai, by suppression subtractive hybridization. Korean J. Malacol., 29:181-187.

38.

Navarro, J.M. and Gonzalez, C.M. (1998) Physiological responses of the chilean scallop Argopecten purpuratus to decreasing salinities. Aquaculture, 167:315-327.

39.

Newell, R.C. and Kofoed, L.H. (1977) Adjustment of the components of energy balance in the gastropod Crepidula fornicate in response to thermal acclimation. Mar. Boil., 44: 275-286.

40.

Paillard, C., Allam, B. and Oubella, R. (2004) Effect of temperature on defense parameters in Manila clam, Ruditapes philippinarum challenged with Vibrio tapes. Dis. Aquat. Org., 59: 249-262.

41.

Parsell, D. and Lindquist, S. (1993) The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins. Annu. Rev. Genet., 27: 437-496.

42.

Richter, K. and Buchner, J. (2001) Hsp90: chaperoning signal transduction. J. Cell Physiol., 188: 281-290.

43.

Shin, Y.K., Kim, Y., Chung, E.Y. and Hur, S.B. (2000)Temperature and salinity tolerance of the Manila clam, Ruditapes philippinarum. Korean J. Fish. Soc., 34: 190-193.

44.

Shin, Y.K., Moon, T.S. and Wi, C.H. (2002) Effects of the dis-solved oxygen concentration on the physiology of the blood cockle, Tegilarca granosa. Korean J. Fish. Soc., 35: 485-489.

45.

Shin, Y.K. and Wi, C.H. (2004) Effect of temperature and salinity on survival and metabolism of the hard shelled mussel Mytilus coruscus, Bivalve: Mytilidae. J. of Aquaculture, 17: 103-108.

46.

Soetaert, A., Moens, L.N., Van der Ven, K., Van Leemput K., Naudts, B., Blust, R. and De Coen, W.M. (2006) Molecular impact of propiconazole on Daphnia magna using a reproduction-related cDNA array. Comp. Biochem. Physiol. C, 142: 66-76.

47.

Song, J.H., Kim, C.H., Park, S.W., Yu, J.H. and Jo, Y.J. (2008) Seasonality of the biological activity factors of the hard clam Meretrix lusoria in the western coast of Korea. J. Aquaculture, 21: 111-122.

48.

Teixeira, T., Diniz, M., Calado, R. and Rosa, R. (2013)Coral physiological adaptations to air exposure: Heat shock and oxidative stress responses in Veretillum cynomorium. Journal of experimental marine biology and ecology, 439: 35-41.

49.

Widdows, J. and Shick, J.M. (1985) Physiological responses of Mytilus edulis and Cardium edule to aerial exposure. Marine Biology, 85: 217-232.

50.

Widdows, J., Bayne, B.L., Livingstone, D.R., Newell, R.I.E. and Domkin, P. (1979) Physiological and biochemical responses of bivalve molluscs to exposure to air. Comp. Biochem. Physiol., 62: 301-308.

51.

Wilson, J.G. and Elkaim, B. (1991) Tolerances to high temperature of infaunal bivalves and the effect of geographical distribution, position on the shore and season. J. Mar. Biol. Ass. U.K., 71: 169-177.

52.

Wu, L.T. and Chu, K.H. (2008) Characterization of heat shock protein 90 in the shrimp Metapenaeus ensis:evidence for its role in the regulation of vitellogenin synthesis. Mol. Reprod. Dev., 75: 952-959.

53.

Xu, Q. and Qin, Y. (2012) Molecular cloning of heat shock protein 60 (PtHSP60) from Portunus trituberculatus and its expression response to salinity stress. Cell Stress Chaperones, 17: 589-601.

54.

Yoo, S.K., Kang, K.H. and Lee, D.Y. (1988) Occurrence and survival rate of sea mussel, Mytilus edulis. Bull. Korean Fish. Soc., 21: 35-41.

55.

You, L., Ning, X., Liu, F., Zhao, J., Wang, Q. and Wu, H. (2013) The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge. Fish Shellfish Immunol., 35:343-350.

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