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A solid-state NMR study on the activity of an antimicrobial peptide, magainin 2
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2011, v.24 no.6, pp.460-466
https://doi.org/10.5806/AST.2011.24.6.460
(2011). A solid-state NMR study on the activity of an antimicrobial peptide, magainin 2. Analytical Science and Technology, 24(6), 460-466, https://doi.org/10.5806/AST.2011.24.6.460
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Abstract
The activity of an antimicrobial peptide, magainin 2, on lipid membranes was investigated using solid-state NMR and a new sampling method that employed mechanically aligned bilayers between thin glass plates. The experiments were performed at two hydration levels. At 95% hydration about 15% of the lipid bilayers were disrupted and at full hydration 20% were disrupted. From the comparison of two equilibrium states established by two sampling methods the importance of peptide binding to the lipid bilayer for whole membrane disruption was demonstrated.
- keywords
- antimicrobial peptide, lipid bilayer, hydration, solid-state NMR
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