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A solid-state NMR study on the activity of an antimicrobial peptide, magainin 2
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2011, v.24 no.6, pp.460-466
https://doi.org/10.5806/AST.2011.24.6.460
(2011). A solid-state NMR study on the activity of an antimicrobial peptide, magainin 2. Analytical Science and Technology, 24(6), 460-466, https://doi.org/10.5806/AST.2011.24.6.460
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Abstract
The activity of an antimicrobial peptide, magainin 2, on lipid membranes was investigated using solid-state NMR and a new sampling method that employed mechanically aligned bilayers between thin glass plates. The experiments were performed at two hydration levels. At 95% hydration about 15% of the lipid bilayers were disrupted and at full hydration 20% were disrupted. From the comparison of two equilibrium states established by two sampling methods the importance of peptide binding to the lipid bilayer for whole membrane disruption was demonstrated.
- keywords
- antimicrobial peptide, lipid bilayer, hydration, solid-state NMR
Reference
1
1. R. Latorre and O. Alvarez, Physiol. Rev., 61, 77-150 (1981).
2
2. H. Steiner, D. Hultmark, A. Engstrom, H. Bennich, H. G. Boman, Nature, 292, 246-248 (1981).
3
3. J. Y. Lee, A. Boman, C. X. Sun, M. Andersson, H. Jornvall, V. Mutt and H. G. Boman, Proc. Natl. Acad. Sci. U.S.A., 86, 9159-9162 (1989).
4
4. K. J. Hallock, D. K. Lee and A. Ramamoorthy, Biophys. J., 84, 3052-3060 (2003).
5
5. P. M Hwang and H. J. Vogel, Biochem. Cell Biol., 76, 235-246 (1998).
6
6. M. Zasloff, Proc. Natl. Acad. Sci. U.S.A., 84, 5449-5453 (1987).
7
7. J. J. Buffy, A. J. Waring and M. Hong, J. Am. Chem. Soc., 127, 4477-4483 (2005).
8
8. M. R. Yeaman and N. Y. Yount, Pharmacol. Rev., 55, 27-55 (2003).
9
9. R. Mani, A. J. Waring, R. I. Lehrer and M. Hong, Biochim. Biophys. Acta, Biomembr., 1716, 11-18 (2005).
10
10. Y. Bai, S. Liu, P. Jiang, L. Zhou, J. Li, C. Tang, C. Verma, Y. Mu, R. W. Beuerman and K. Pervushin, Biochemistry, 48, 7229-7239 (2009).
11
11. O. Toke, Biopolymers, 80, 717-735 (2005).
12
12. A. Pokorny and P. F. F. Almeida, Biochemistry, 44, 9538-9544 (2005).
13
13. K. Matsuzaki, O. Murase, N. Fujii and K. Miyajima, Biochemistry, 34, 6521-6526 (1995).
14
14. K. Matsuzaki, K.-I. Sugishita, N. Ishibe, M. Ueha, S. Nakata, K. Miyajima and R. M. Epand, Biochemistry 37, 11856-11863 (1998).
15
15. Y. Herasimenka, M. Benincasa, M. Mattiuzzo, P. Cescutti, R. Gennaro and R. Rizzo, Peptides, 26, 1127-1132 (2005).
16
16. J.-A. Richard, I. Kelly, D. Marion, M. Pezolet and M. Auger, Biophys. J., 83, 2074-2083 (2002).
17
17. S. Yamaguchi, T. Hong, A. Waring, R. I. Lehrer, M. Hong, Biochemistry, 41, 9852-9862 (2002).
18
18. B. Bechinger, Biochim. Biophys. Acta, Biomembr., 1712, 101-108 (2005).
19
19. R. Mani, M. Tang, X. Wu, J. J. Buffy, A. J. Waring, M. A. Sherman and M. Hong, Biochemistry, 45, 8341-8349 (2006).
20
20. J. J. Buffy, M. J. McCormick, S. Wi, A. Waring, R. I. Lehrer and M. Hong, Biochemistry, 43, 9800-9812 (2004).
21
21. S. Wi and C. Kim, J. Phys. Chem. B, 112, 11402-11414 (2008).
22
22. C. Kim and S. Wi, Bull. Korean Chem. Soc., in press, (2012).
23
23. K. Matsuzaki, O. Murase and K. Miyajima, Biochemistry, 34, 12553-12559 (1995).
24
24. S. J. Ludtke, K. He, Y. Wu and H. W. Huang, Biochim. Biophys. Acta, Biomembr., 1190, 181-184 (1994).
25
25. R. Mani, J. J. Buffy, A. J. Waring, R. I. Lehrer and M. Hong, Biochemistry, 43, 13839-13848 (2004).
26
26. K. J. Hallock, D.-K. Lee, J. Omnaas, H. I. Mosberg and A. Ramamoorthy, Biophys. J., 83, 1004-1013(2002).
27
27. E. W. Washburn, C. J. West and C. Hull, International Critical Tables of Numerical Data, Physics, Chemistry, and Technology; McGraw-Hill: New York, 1926.
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